Recombinant Human Wnt-16b Protein, CF

Images

 
There are currently no images for Wnt-16b (7790-WN/CF).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Recombinant Human Wnt-16b Protein, CF Summary

Details of Functionality
Measured in a cell proliferation/survival assay using 3T3‑L1 mouse embryonic fibroblast adipose-like cells. The ED50 for this effect is 0.4‑1.6 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human Wnt-16b protein
Asn30-Lys365
Accession #
N-terminal Sequence
Asn30
Protein/Peptide Type
Recombinant Proteins
Gene
WNT16
Purity
>80%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
37.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
45-65 kDa, reducing conditions
Publications
Read Publications using
7790-WN/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and CHAPS.
Purity
>80%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 200 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Wnt-16b Protein, CF

  • WNT16
  • Wnt16b
  • Wnt-16b

Background

Wnt‑16 is a 40 kDa protein within the Wnt family of secreted, highly conserved, cysteine‑rich, palmitoylated cell signaling glycoproteins that play important roles in vertebrate developmental pattern formation, cell fate decision, axon guidance, and tumor formation (1‑3). Wnt‑16a and Wnt‑16b isoforms in humans differ in the signal sequence and the first two amino acids (aa) of the mature protein (2, 3). Wnt‑16b is the more conserved isoform and is widely expressed, while Wnt‑16a is expressed mainly in the human pancreas (3). Mature human Wnt‑16b shares 92%, 93%, and 95% aa sequence identity with mouse/rat, rabbit/porcine/equine, and bovine Wnt‑16, respectively. Wnt‑16 expression is detected on uterine stroma adjacent to the luminal epithelium during implantation (4). It is up‑regulated during the first embryonic lymphoid progenitor differentiation (5). Congenital heart defects correlate with elevated Wnt‑16 in mouse embryos and human amniotic fluid (6). Low cortical bone thickness and bone mineral density correlate with deletion of Wnt‑16 in mice and a Wnt‑16 missense SNP in humans (7). Wnt‑16 is over‑expressed in cells undergoing replicative senescence, and is up‑regulated in articular cartilage by injury and osteoarthritis (8, 9). Wnt‑16b expression in skin is up‑regulated in human basal cell carcinomas, enhancing cell survival (10). Its expression is also up‑regulated by DNA damage (radiation and chemotherapy) in stroma surrounding prostate tumors, causing enhanced survival and treatment resistance in the tumor cells (11). Pre‑B acute lymphoblastic leukemia with t(1;19) translocation, creating an E2A‑Pbx1 fusion protein, also causes up‑regulation of Wnt‑16 that confers resistance to apoptosis (12, 13). Wnt‑16 signaling through both canonical and JNK‑mediated (non‑canonical) pathways is reported (8‑10).
  1. Clevers, H. and R. Nusse (2012) Cell 149:1192.
  2. Katoh, H. and M. Katoh 2005) Oncol. Rep. 13:771.
  3. Fear, M.W. et al. (2000) Biochem. Biophys. Res. Commun. 278:814.
  4. Hayashi, K. et al. (2009) Biol. Reprod. 80:989.
  5. Corrigan, P.M. et al. (2009) Stem Cells Dev. 18:759.
  6. Nath, A.K. et al. (2009) PLoS ONE 4:e4221.
  7. Zheng, H.F. et al. (2012) PLoS Genet. 8:31002745.
  8. Dell’accio, F. et al. (2008) Arthritis Rheum. 58:1410.
  9. Binet, R. et al. (2009) Cancer Res. 69:9183.
  10. Teh, M.T. et al. (2006) J. Cell Sci. 120:330.
  11. Sun, Y. et al. (2012) Nat. Med. 18:1359.
  12. McWhirter, J.R. et al. (1999) Proc. Natl. Acad. Sci. USA 96:11464.
  13. Mazieres, J. et al. (2005) Oncogene 24:5396.

Customers Who Viewed This Item Also Viewed...

AF475
Species: Mu
Applications: WB, IHC
AF3157
Species: Hu
Applications: WB, IHC
NBP2-20913
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NBP1-54467
Species: Hu, Mu, Rt, Ch, Pm
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC
6179-WN/CF
Species: Hu
AF3460
Species: Hu
Applications: WB, IHC
NBP2-75713
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
645-WN
3669-WN/CF
Species: Mu
AF3464
Species: Hu
Applications: WB, IHC
NBP2-46367
Species: Hu
Applications: WB, IHC
AF4109
Species: Hu
Applications: WB, IHC
NBP1-76916
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
7196-WN/CF
Species: Hu
NBP1-54467
Species: Hu, Mu, Rt, Ch, Pm
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC
NBP2-29463
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-24695
Species: Hu
Applications: WB, IHC, IHC-P
7790-WN/CF
Species: Hu
Applications: Bioactivity

Publications for Wnt-16b (7790-WN/CF)(4)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: Bioassay, In Vivo.


Filter By Application
Bioassay
(3)
In Vivo
(1)
All Applications
Filter By Species
Human
(3)
Mouse
(1)
All Species

Reviews for Wnt-16b (7790-WN/CF) (0)

There are no reviews for Wnt-16b (7790-WN/CF). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for Wnt-16b (7790-WN/CF) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

Additional Wnt-16b Products

Bioinformatics Tool for Wnt-16b (7790-WN/CF)

Discover related pathways, diseases and genes to Wnt-16b (7790-WN/CF). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Wnt-16b (7790-WN/CF)

Discover more about diseases related to Wnt-16b (7790-WN/CF).
 

Pathways for Wnt-16b (7790-WN/CF)

View related products by pathway.

PTMs for Wnt-16b (7790-WN/CF)

Learn more about PTMs related to Wnt-16b (7790-WN/CF).

Blogs on Wnt-16b

There are no specific blogs for Wnt-16b, but you can read our latest blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

Wnt-5a
645-WN-010

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human Wnt-16b Protein, CF and receive a gift card or discount.

Bioinformatics

Gene Symbol WNT16
Uniprot