Recombinant Human Wnt-11 Protein, CF

Images

 
There are currently no images for Wnt-11 (6179-WN/CF).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Recombinant Human Wnt-11 Protein, CF Summary

Details of Functionality
Measured in a cell proliferation/survival assay using IEC‑18 rat small intestinal epithelial cells. The ED50 for this effect is 0.6-2.4 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human Wnt-11 protein
Ile25-Lys354
Accession #
N-terminal Sequence
Ile25
Protein/Peptide Type
Recombinant Proteins
Gene
WNT11
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
36.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
38-40 kDa, reducing conditions
Publications
Read Publications using
6179-WN/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and CHAPS.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Wnt-11 Protein, CF

  • HWNT11
  • MGC141946
  • MGC141948
  • protein Wnt-11
  • wingless-type MMTV integration site family, member 11
  • Wnt11
  • Wnt-11

Background

Wnt‑11 belongs to the Wnt family of secreted, highly conserved, cysteine-rich cell signaling glycoproteins that play important roles in vertebrate developmental pattern formation, cell fate decision, axon guidance, and tumor formation (1-3). Mature human Wnt‑11 shares 98% amino acid (aa) sequence identity with mouse, rat and bovine Wnt‑11, and 99%, 96% and 88% aa identity with canine, equine and chick Wnt‑11, respectively. Mouse Wnt‑11 is expressed in early development during gastrulation and in the perichondrium, lung mesenchyme and ureteric buds, but not in neuroepithelium (1). It is required for cardiogenesis, organization of early muscle fibers, and branching morphogenesis of ureters (3-7). It is also expressed in several human cancers (2). In vitro, Wnt‑11 has been shown to induce cardiomyocyte differentiation from unfractionated mouse bone marrow mononuclear cells, and to promote hematopoietic differentiation from human embryoid body stem cells by engaging Frizzled-7 in the presence of E-cadherin (6, 8). In general, there are three signaling pathways associated with Wnt‑receptor interaction. The canonical pathway results in beta -Catenin accumulation and TCF/LEF‑1‑mediated gene transcription. Non‑canonical pathways include the Wnt/Ca2+ pathway and the planar cell polarity (PCP) pathway. Wnt‑11 often, but not exclusively, signals by a non‑canonical pathway and may repress canonical signaling by other Wnts (2, 9). However, when complexed with Wnt‑5a, Wnt‑11 can also enhance canonical signaling (10).

  1. Lako, M. et al. (1998) Gene 219:101.
  2. Katoh, M. and M. Katoh (2009) Int. J. Mol. Med. 24:247.
  3. Flaherty, M.P. and B. Dawn (2008) Trends Cardiovasc. Med. 18:260.
  4. Gros, J. et al. (2009) Nature 457:589.
  5. Pandur, P. et al. (2002) Nature 418:636.
  6. Flaherty, M.P. et al. (2008) Circulation 117:2241.
  7. Majumdar, A. et al. (2003) Development 130:3175.
  8. Vijayaragavan, K. et al. (2009) Cell Stem Cell 4:248.
  9. Maye, P. et al. (2004) J. Biol. Chem. 279:24659.
  10. Cha, S-W. et al. (2009) Curr. Biol. 19:1573.

Customers Who Viewed This Item Also Viewed...

645-WN/CF
Species: Hu, Mu
Applications: BA
NBP1-54467
Species: Ch, Av-Du, Hu, Mu, Pm, Rt
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-P, IP, Simple Western, WB
5036-WN/CF
Species: Hu
Applications: BA, BA
3669-WN/CF
Species: Mu
Applications: BA
AF475
Species: Mu
Applications: IHC, WB
6179-WN/CF
Species: Hu
Applications: BA
7347-WN/CF
Species: Hu
Applications: BA
NBP2-75713
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NBP2-46367
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
212-GD/CF
Species: Hu
Applications: Bind, BA
AF3157
Species: Hu
Applications: IHC, WB
AF3460
Species: Hu
Applications: IHC, WB
8419-WN/CF
Species: Mu
Applications: BA
AF1927
Species: Hu
Applications: IP, WB
7196-WN/CF
Species: Hu
Applications: BA
AF3464
Species: Hu
Applications: IHC, WB
NBP2-20913
Species: Hu, Mu
Applications: IHC, IHC-P, WB
AF6278
Species: Hu, Mu
Applications: WB
AF4109
Species: Hu
Applications: IHC, WB
6179-WN/CF
Species: Hu
Applications: Bioactivity

Publications for Wnt-11 (6179-WN/CF)(18)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Transgenic Mouse, Zebrafish.

We have publications tested in 4 applications: Bioassay, Differentiation, In Vivo, Western Blot.


Filter By Application
Bioassay
(12)
Differentiation
(2)
In Vivo
(2)
Western Blot
(1)
All Applications
Filter By Species
Human
(9)
Mouse
(6)
Rat
(1)
Transgenic Mouse
(1)
Zebrafish
(1)
All Species
Showing Publications 1 - 10 of 18. Show All 18 Publications.
Publications using 6179-WN/CF Applications Species
N Salerno, F Marino, M Scalise, L Salerno, C Molinaro, A Filardo, A Chiefalo, G Panuccio, A De Angelis, K Urbanek, D Torella, E Cianflone Pharmacological clearance of senescent cells improves cardiac remodeling and function after myocardial infarction in female aged mice Mechanisms of Ageing and Development, 2022;208(0):111740. 2022 [PMID: 36150603] (Differentiation, Mouse) Differentiation Mouse
AK Heilig, R Nakamura, A Shimada, Y Hashimoto, Y Nakamura, J Wittbrodt, H Takeda, T Kawanishi Wnt11 acts on dermomyotome cells to guide epaxial myotome morphogenesis Elife, 2022;11(0):. 2022 [PMID: 35522214] (In Vivo, Zebrafish) In Vivo Zebrafish
B ?wierczek-, D Dudka, D Bauer, T Czajkowski, K Ilach, W Streminska, A Kominek, K Piwocka, MA Ciemerych, K Archacka Comparison of Differentiation Pattern and WNT/SHH Signaling in Pluripotent Stem Cells Cultured under Different Conditions Cells, 2021;10(10):. 2021 [PMID: 34685722] (Bioassay, Mouse) Bioassay Mouse
ED Arisan, O Rencuzogul, C Cieza-Borr, F Miralles A, M Dwek, S Lange, P Uysal-Onga MiR-21 Is Required for the Epithelial-Mesenchymal Transition in MDA-MB-231 Breast Cancer Cells International Journal of Molecular Sciences, 2021;22(4):. 2021 [PMID: 33557112] (Western Blot, Human) Western Blot Human
A Böttcher, M Büttner, S Tritschler, M Sterr, A Aliluev, L Oppenlände, I Burtscher, S Sass, M Irmler, J Beckers, C Ziegenhain, W Enard, AC Schamberge, FM Verhamme, O Eickelberg, FJ Theis, H Lickert Non-canonical Wnt/PCP signalling regulates intestinal stem cell lineage priming towards enteroendocrine and Paneth cell fates Nature Cell Biology, 2021;23(1):23-31. 2021 [PMID: 33398177] (Bioassay, Transgenic Mouse) Bioassay Transgenic Mouse
I Rodriguez-, O Maiques, L Kohlhammer, G Cantelli, A Perdrix-Ro, J Monger, B Fanshawe, VL Bridgeman, SN Karagianni, RM Penin, J Marcolval, RM Marti, X Matias-Gui, GO Fruhwirth, JL Orgaz, I Malanchi, V Sanz-Moren WNT11-FZD7-DAAM1 signalling supports tumour initiating abilities and melanoma amoeboid invasion Nat Commun, 2020;11(1):5315. 2020 [PMID: 33082334] (Bioassay, Human) Bioassay Human
W Liang, P Han, EH Kim, J Mak, R Zhang, AG Torrente, JI Goldhaber, E Marbán, HC Cho Canonical Wnt signaling promotes pacemaker cell specification of cardiac mesodermal cells derived from mouse and human embryonic stem cells Stem Cells, 2019;0(0):. 2019 [PMID: 31648393] (Bioassay, Mouse) Bioassay Mouse
P Andersen, E Tampakakis, DV Jimenez, S Kannan, M Miyamoto, HK Shin, A Saberi, S Murphy, E Sulistio, SP Chelko, C Kwon Precardiac organoids form two heart fields via Bmp/Wnt signaling Nat Commun, 2018;9(1):3140. 2018 [PMID: 30087351] (Mouse) Mouse
BD Boyan, R Olivares-N, MB Berger, SL Hyzy, Z Schwartz Role of Wnt11 during Osteogenic Differentiation of Human Mesenchymal Stem Cells on Microstructured Titanium Surfaces Sci Rep, 2018;8(1):8588. 2018 [PMID: 29872092] (Bioassay, Human) Bioassay Human
DE Reichman, L Park, L Man, D Redmond, K Chao, RP Harvey, MM Taketo, Z Rosenwaks, D James Wnt inhibition promotes vascular specification of embryonic cardiac progenitors Development, 2018;145(1):. 2018 [PMID: 29217753] (Differentiation, Human) Differentiation Human
Show All 18 Publications.

Reviews for Wnt-11 (6179-WN/CF) (0)

There are no reviews for Wnt-11 (6179-WN/CF). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for Wnt-11 (6179-WN/CF) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.
Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human Wnt-11 Protein, CF and receive a gift card or discount.

Bioinformatics

Gene Symbol WNT11
Uniprot