Recombinant Human Wnt-11 Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human Wnt-11 Protein, CF Summary

Details of Functionality
Measured in a cell proliferation/survival assay using IEC‑18 rat small intestinal epithelial cells. The ED50 for this effect is 0.6-2.4 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human Wnt-11 protein
Ile25-Lys354
Accession #
N-terminal Sequence
Ile25
Protein/Peptide Type
Recombinant Proteins
Gene
WNT11
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
36.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
38-40 kDa, reducing conditions
Publications
Read Publications using
6179-WN/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and CHAPS.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Wnt-11 Protein, CF

  • HWNT11
  • MGC141946
  • MGC141948
  • protein Wnt-11
  • wingless-type MMTV integration site family, member 11
  • Wnt11
  • Wnt-11

Background

Wnt‑11 belongs to the Wnt family of secreted, highly conserved, cysteine-rich cell signaling glycoproteins that play important roles in vertebrate developmental pattern formation, cell fate decision, axon guidance, and tumor formation (1-3). Mature human Wnt‑11 shares 98% amino acid (aa) sequence identity with mouse, rat and bovine Wnt‑11, and 99%, 96% and 88% aa identity with canine, equine and chick Wnt‑11, respectively. Mouse Wnt‑11 is expressed in early development during gastrulation and in the perichondrium, lung mesenchyme and ureteric buds, but not in neuroepithelium (1). It is required for cardiogenesis, organization of early muscle fibers, and branching morphogenesis of ureters (3-7). It is also expressed in several human cancers (2). In vitro, Wnt‑11 has been shown to induce cardiomyocyte differentiation from unfractionated mouse bone marrow mononuclear cells, and to promote hematopoietic differentiation from human embryoid body stem cells by engaging Frizzled-7 in the presence of E-cadherin (6, 8). In general, there are three signaling pathways associated with Wnt‑receptor interaction. The canonical pathway results in beta -Catenin accumulation and TCF/LEF‑1‑mediated gene transcription. Non‑canonical pathways include the Wnt/Ca2+ pathway and the planar cell polarity (PCP) pathway. Wnt‑11 often, but not exclusively, signals by a non‑canonical pathway and may repress canonical signaling by other Wnts (2, 9). However, when complexed with Wnt‑5a, Wnt‑11 can also enhance canonical signaling (10).

  1. Lako, M. et al. (1998) Gene 219:101.
  2. Katoh, M. and M. Katoh (2009) Int. J. Mol. Med. 24:247.
  3. Flaherty, M.P. and B. Dawn (2008) Trends Cardiovasc. Med. 18:260.
  4. Gros, J. et al. (2009) Nature 457:589.
  5. Pandur, P. et al. (2002) Nature 418:636.
  6. Flaherty, M.P. et al. (2008) Circulation 117:2241.
  7. Majumdar, A. et al. (2003) Development 130:3175.
  8. Vijayaragavan, K. et al. (2009) Cell Stem Cell 4:248.
  9. Maye, P. et al. (2004) J. Biol. Chem. 279:24659.
  10. Cha, S-W. et al. (2009) Curr. Biol. 19:1573.

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Publications for Wnt-11 (6179-WN/CF)(12)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: Bioassay, Differentiation, In Vivo.


Filter By Application
Bioassay
(9)
Differentiation
(1)
In Vivo
(1)
All Applications
Filter By Species
Human
(7)
Mouse
(4)
Rat
(1)
All Species
Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using 6179-WN/CF Applications Species
W Liang, P Han, EH Kim, J Mak, R Zhang, AG Torrente, JI Goldhaber, E Marbán, HC Cho Canonical Wnt signaling promotes pacemaker cell specification of cardiac mesodermal cells derived from mouse and human embryonic stem cells Stem Cells, 2019;0(0):. 2019 [PMID: 31648393] (Bioassay, Mouse) Bioassay Mouse
P Andersen, E Tampakakis, DV Jimenez, S Kannan, M Miyamoto, HK Shin, A Saberi, S Murphy, E Sulistio, SP Chelko, C Kwon Precardiac organoids form two heart fields via Bmp/Wnt signaling Nat Commun, 2018;9(1):3140. 2018 [PMID: 30087351] (Mouse) Mouse
BD Boyan, R Olivares-N, MB Berger, SL Hyzy, Z Schwartz Role of Wnt11 during Osteogenic Differentiation of Human Mesenchymal Stem Cells on Microstructured Titanium Surfaces Sci Rep, 2018;8(1):8588. 2018 [PMID: 29872092] (Bioassay, Human) Bioassay Human
DE Reichman, L Park, L Man, D Redmond, K Chao, RP Harvey, MM Taketo, Z Rosenwaks, D James Wnt inhibition promotes vascular specification of embryonic cardiac progenitors Development, 2018;145(1):. 2018 [PMID: 29217753] (Differentiation, Human) Differentiation Human
M Touma, X Kang, F Gao, Y Zhao, AA Cass, R Biniwale, X Xiao, M Eghbali, G Coppola, B Reemtsen, Y Wang Wnt11 regulates cardiac chamber development and disease during perinatal maturation JCI Insight, 2017;2(17):. 2017 [PMID: 28878122] (Bioassay, Rat) Bioassay Rat
J Messéant, J Ezan, P Delers, K Glebov, C Marchiol, F Lager, G Renault, F Tissir, M Montcouqui, N Sans, C Legay, L Strochlic Wnts contribute to neuromuscular junction formation through distinct signaling pathways Development, 2017;0(0):. 2017 [PMID: 28348167] (In Vivo, Mouse) In Vivo Mouse
Increased Wnt5a in squamous cell lung carcinoma inhibits endothelial cell motility BMC Cancer, 2016;16(1):915. 2016 [PMID: 27876017] (Bioassay, Human) Bioassay Human
Kilander M, Dahlstrom J, Schulte G Assessment of Frizzled 6 membrane mobility by FRAP supports G protein coupling and reveals WNT-Frizzled selectivity. Cell Signal, 2014;26(9):1943-9. 2014 [PMID: 24873871] (Bioassay, Human) Bioassay Human
Keats E, Dominguez J, Grant M, Khan Z Switch from canonical to noncanonical Wnt signaling mediates high glucose-induced adipogenesis. Stem Cells, 2014;32(6):1649-60. 2014 [PMID: 24496952] (Bioassay, Human) Bioassay Human
Thrasivoulou C, Millar M, Ahmed A Activation of intracellular calcium by multiple Wnt ligands and translocation of beta-catenin into the nucleus: a convergent model of Wnt/Ca2+ and Wnt/beta-catenin pathways. J Biol Chem, 2013;288(50):35651-9. 2013 [PMID: 24158438] (Bioassay, Human) Bioassay Human
Show All 12 Publications.

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Bioinformatics

Gene Symbol WNT11
Uniprot