Recombinant Human UNC5H1 Fc Chimera Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human UNC5H1 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA.

  When Recombinant Human Netrin-1 (Catalog # 6419-N1) is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Human UNC5H1 Fc Chimera that produces 50% of the optimal binding response is found to be approximately 0.3‑1.5 μg/mL.

• Source: Mouse myeloma cell line, NS0-derived human UNC5H1 protein
  

  Human UNC5H1 (Gln26 - Tyr306) Accession # NP_588610	IEGRMD	Human IgG1 (Pro100 - Lys330)
  N-terminus		C-terminus

• Accession #: NP_588610
• N-terminal Sequence: No results obtained: Gln26 predicted
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: UNC5A
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 57.9 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 80-90 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human UNC5H1 Fc Chimera Protein, CF

• KIAA1976netrin receptor UNC5A
• netrin receptor Unc5h1
• Protein unc-5 homolog 1
• Protein unc-5 homolog A
• unc5 (C.elegans homolog) a
• unc-5 homolog 1
• unc-5 homolog A (C. elegans)
• UNC5A
• UNC5H1
• UNC5H1FLJ16449

Background

UNC5H1, also known as UNC5A, is a member of the UNC5 (Drosophila uncoordinated-5 homology) family of type I transmembrane proteins within the immunoglobulin (Ig) superfamily. UNC5H1-4 (UNC5A-D, respectively) have two Ig and one or two thrombospondin type 1 (TSP1) domains in their extracellular regions, and a ZU-5 domain, a DCC (Deleted in Colorectal Cancer)-binding domain (DB) and a C-terminal death domain (DD) in their cytoplasmic regions (1, 2). Human UNC5H1 cDNA encodes 842 amino acids (aa) including a 25 aa signal peptide, a 281 aa extracellular domain with one TSP1 domain, and a 515 aa cytoplasmic domain. Within the extracellular domain, human UNC5A shares approximately 99%, 99%, 98%, 98%, 97% and 96% aa sequence identity with mouse, equine, rat, canine, bovine and porcine one-TSP1 isoforms of UNC5H1. UNC5 proteins are receptors for the netrin/UNC6 family of laminin-related secreted axon guidance cues; UNC5H1 has been shown to bind netrin 1, 3 and 4 (2 ‑ 4). Netrins can act as a chemoattractant for some axons in the presence of DCC (Deleted in Colorectal Cancer) proteins. However, UNC5H1 association with netrins changes them to chemorepellents (1, 5, 6). UNC5H1 is expressed after developmental migration of motor neurons, presumably halting their migration (7). UNC5H1 can be removed from the cell surface via endocytosis following phosphorylation by protein kinase C (PKC) in complex with PICK1. In turn, PKC activity is regulated by the neuronal G protein-coupled adenosine receptor A2b R (6, 8, 9). The UNC5 and DCC families also act as dependence receptors, and are pro-apoptotic in the absence of netrins (10 ‑ 13). UNC5H1 transcription is enhanced by the tumor suppressor p53, allowing increased apoptosis of tumor cells in the absence of netrin and the presence of NRAGE (13, 14).

1. Moore, S.W. et al. (2007) Adv. Exp. Med. Biol. 621:17.
2. Leonardo, E.D. et al. (1997) Nature 386:833.
3. Wang, H. et al. (1999) J. Neurosci. 19:4938.
4. Qin, S. et al. (2007) Mol. Cell. Neurosci. 34:243.
5. Hong, K. et al. (1999) Cell 97:927.
6. Bartoe, J.L. et al. (2006) J. Neurosci. 26:3192.
7. Barrett, C. and S. Guthrie (2001) Mech. Dev. 106:163.
8. Williams, M.E. et al. (2003) J. Neurosci. 23:11279.
9. McKenna, W.L. et al. (2008) J. Neurochem. 104:1081.
10. Llambi, F. et al. (2001) EMBO J. 20:2715.
11. Thiebault, K. et al. (2003) Proc. Natl. Acad. Sci. USA 100:4173.
12. Williams, M.E. et al. (2006) Nat. Neurosci. 9:996.
13. Miyamoto, Y. et al. (2010) Int. J. Oncol. 36:1253.
14. Williams, M.E. et al. (2003) J. Biol. Chem. 278:17483.

Bioinformatics

• Gene Symbol: UNC5A
• Uniprot:
  • NP_588610()