Recombinant Human TSLPR Fc Chimera Avi-tag Protein, CF

When Human TSLP Protein (Catalog # 1398-TS)is immobilized at 0.5 μg/mL (100 μL/well), it binds to Biotinylated Recombinant Human TSLPR Fc Chimera Avi-tag (Catalog # AVI981) with an ED50 of 0.75‑7.5 ng/mL.

2 μg/lane of Biotinylated Recombinant Human TSLPR Fc Chimera Avi-tag (Catalog # AVI981) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 65-80 kDa and 130-160 kDa, respectively.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human TSLPR Fc Chimera Avi-tag Protein, CF Summary

• Additional Information: Biotinylated
• Details of Functionality: The biotin to protein ratio is greater than 0.7 as determined by the HABA assay. Measured by its binding ability in a functional ELISA. When Recombinant Human TSLP (Catalog # 1398-TS) is immobilized at 0.5 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human TSLP Fc Chimera Avi-tag (Catalog # AVI981) that produces 50% of the optimal binding response is 0.75-7.5 ng/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived human TSLPR protein
  

  Human TSLPR (Gly25 & Val30-Lys231) Accession # Q9HC73.1	DIEGRMD	Human IgG1 (Pro100-Lys330)	Avi-tag
  N-terminus			C-terminus

• Accession #: Q9HC73.1
• N-terminal Sequence: Gly25 and Val30
• Structure / Form: Biotinylated via Avi-tag
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • Bioactivity2
• Theoretical MW: 52 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 65-80 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human TSLPR Fc Chimera Avi-tag Protein, CF

• CRL2
• CRL2cytokine receptor CRL2 precusor
• CRLF2
• CRLF2Y
• CRLM-2
• Cytokine receptor-like 2
• cytokine receptor-like factor 2
• ILXR
• IL-XR
• P2RY8/CRLF2 fusion
• PCOR1
• Thymic stromal lymphopoietin protein receptor
• thymic stromal lymphopoietin receptor
• thymic stromal-derived lymphopoietin receptor
• TSLP R
• TSLP receptor
• TSLPR

Background

Thymic stromal lymphopoietin receptor (TSLPR), also known cytokine receptor-like factor 2 and IL-XR, is a member of the type 1 cytokine receptor family. TSLPR has been identified most closely related to the common gamma chain ( gamma c) and, when complexed with interleukin 7 receptor alpha (IL-7R alpha ), forms a high affinity complex for the IL-7-like cytokine TSLP (1-4). The extracellular domain (ECD) of human TSLPR contains two fibronectin type III-like domains and a WSXWS-like motif, which is necessary for proper protein folding (1-4). The cytoplasmic domain contains a membrane-proximal box 1 motif that is important for association with JAKs (1, 2). Human TSLPR shares 34% amino acid sequence identity with mouse TSLPR. An alternatively spliced mRNA variant encoding a soluble TSLPR has also been reported in mouse (5). In the signaling pathway of TSLP-TSLPR, which is similar to that of IL-7, TSLP activates the transcription factor signal transducer and activator of transcription 3 (STAT3), inducing the expression of common genes (4). TSLPR expression is ubiquitous in the immune and hematopoietic cells but is up‑regulated in Th2-skewed cells (3, 4). Elevated expression of TSLP-TSLPR in bronchial mucosa has been associated with human asthma by acting as a susceptibility factor to generate Th2 allergic responses to antigens (4, 6). TSLP also is involved in Th2-mediated allergic skin inflammation by inducing Th2 cytokine secretion by T cells during the effector phase of allergic skin inflammation (4, 7). TSLP has been shown to induce the release of T cell-attracting chemokines from monocytes and enhance the maturation of CD11c+ dendritic cells (DC) (4). TSLP activated human DCs are also involved in the homeostatic proliferation of naïve and memory T cells in the absence of foreign antigens (4).

1. Park LS et al. (2000) J. Exp. Med. 192:659.
2. Blagoev, B. et al. (2002) Gene. 284:161.
3. Ziegler, S.F. et al. (2013) Advances in Pharmacology. 66:129.
4. He, R. et al. (2010) Ann N Y Acad Sci. 1183:13.
5. Hiroyama, T. et al. (2000) Biochem. Biophys. Res. Commun. 272:224.
6. Headley, M.B. et al. (2009) J Imunol. 182:1641.
7. Soumelis, V. et al. (2002) Nat Immunol. 3:673.

Bioinformatics

• Uniprot:
  • Q9HC73.1()