Recombinant Human THSD7A Protein, CF

Immobilized Recombinant Human THSD7A (Catalog # 9524-TH)supports the adhesion of SVEC4‑10 mouse vascular endothelial cells. The ED50 for thiseffect is 0.1‑0.6 μg/mL.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human THSD7A Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of SVEC4‑10 mouse vascular endothelial cells. The ED₅₀ for this effect is 0.1-0.6 μg/mL.
• Source: Human embryonic kidney cell, HEK293-derived human THSD7A protein
  Ala48-Trp1607, with a C-terminal 6-His tag
• Accession #: Q9UPZ6
• N-terminal Sequence: Ala48
• Protein/Peptide Type: Recombinant Proteins
• Purity: >85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 176 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 160-265 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human THSD7A Protein, CF

• Gm837
• KIAA0960
• thrombospondin, type I, domain containing 7A
• THSD7A

Background

Thrombospondin type I domain containing 7A, also known as THSD7A, is an approximately 250 kDa type I membrane protein (1). THSD-7A has a large extracellular domain containing ten thrombospondin type 1 repeats, six WSXW motifs, one RGD motif, and fourteen predicted N‑glycosylated sites (1). Mature THSD7A shares 91% and 92% amino acid sequence identity with mouse and rat, respectively. THSD7A is expressed in podocytes, glomerular endothelial cells and mesangial cells (2). It is a novel neural protein known to affect endothelial migration and vascular patterning during development (1, 3). Soluble THSD7A promotes endothelial filopodia formation and focal adhesion assembly and induces FAK-dependent signaling during angiogenesis (1). THSD7A can co-localize with aVb3 integrin in HUVECs (3). Additionally, most recent study has indicated that THSD7A is associated with obesity (4).

1. Kuo, M.W. et al. (2011) PLoS One 6: e29000.
2. Tomas, N.M. et al. (2014) N. Engl. J. Med. 371:2277.
3. Wang, C.H. et al. (2010) J. Cell Physiol. 222: 685.
4. Nizamuddin, S. et al. (2015) Int. J. Obes. (Lond.) 39:1662.

Bioinformatics

• Uniprot:
  • Q9UPZ6()