Recombinant Human Thrombospondin-1 Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human Thrombospondin-1 Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to support the adhesion of SVEC4‑10 mouse vascular endothelial cells. When 2 x 104 cells/well are added to DTT treated THBS-1 coated plates (10 µg/mL, 100 µL/well), > 50% will adhere after 1 hour at 37 °C.
Source
Mouse myeloma cell line, NS0-derived human Thrombospondin-1 protein
Asn19-Pro1170, with Thr523Ala substitution and a C-terminal 10-His tag
Accession #
N-terminal Sequence
Asn19
Protein/Peptide Type
Recombinant Proteins
Gene
THBS1
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
129 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
135 - 165 kDa, reducing conditions
Publications
Read Publications using
3074-TH in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in TBS, NaCl, and Trehalose.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Thrombospondin-1 Protein, CF

  • THBS
  • THBS1
  • THBS-1
  • thrombospondin 1
  • Thrombospondin1
  • Thrombospondin-1
  • thrombospondin-1p180
  • TSP-1
  • TSP1thrombospondin-1
  • TSPTSP-1

Background

Thrombospondin-1 (TSP-1) is a 150-180 kDa calcium-sensitive protein that is secreted as a disulfide-linked homotrimer. Within the Thrombospondin family, TSP-2 is also homotrimeric, while TSP-3, -4, and -5/COMP are homopentameric. TSP-1 regulates a wide range of cellular functions including their interactions with other cells and with the extracellular matrix (ECM). TSP-1 contains an N-terminal Laminin G-like globular domain, an extended central region with one vWFC domain, 3 TSP type 1 domains, 2 EGF-like domains, and 8 TSP type-3 domains, and a globular TSP C-terminal domain. Distinct regions of TSP-1 have been associated with binding to particular ECM or cellular molecules (1, 2). Mature human TSP-1 shares 95% amino acid sequence identity with mouse and rat TSP-1. TSP-1 counteracts the angiogenic, hypotensive, and anti-thrombotic effects of nitric oxide (NO) (3). It binds and neutralizes VEGF, blocks VEGF R2 signaling on vascular endothelial cells (EC), and destabilizes adhesive contacts between EC (4-6). TSP-1’s actions on vascular smooth muscle cells support arterial tone and blood pressure in the presence of vasodilatory stimulation (7). It is released from activated platelets and enhances platelet aggregation and adhesion (8). TSP-1 also plays an important role in wound repair and tissue fibrosis by binding latent TGF-beta and inducing release of the active cytokine from the latency associated peptide (LAP) (9, 10). TSP-1 dampens adaptive immune responses by inducing the differentiation of regulatory T cells and inhibiting TCR signaling (11, 12). In the nervous system, it promotes excitatory synapse formation (13, 14) and supports the integration of neural progenitor cells into the olfactory bulb (15). In addition, TSP-1 is released by apoptotic cells and promotes macrophage-mediated debris clearance (16).
  1. Murphy-Ullrich, J.E. and R.V. Iozzo (2012) Matrix Biol. 31:152.
  2. Lawler, J. and R.O. Hynes (1986) J. Cell Biol. 103:1635.
  3. Roberts, D.D. et al. (2012) Matrix Biol. 31:162.
  4. Gupta, K. et al. (1999) Angiogenesis 3:147.
  5. Kaur, S. et al. (2010) J. Biol. Chem. 285:38923.
  6. Garg, P. et al. (2011) Am. J. Physiol. Lung Cell. Mol. Physiol. 301:L79.
  7. Isenberg, J.S. et al. (2009) Matrix Biol. 28:110.
  8. Isenberg, J.S. et al. (2008) Blood 111:613.
  9. Schultz-Cherry, S. et al. (1994) J. Biol. Chem. 269:26775.
  10. Sweetwyne, M.T. and J.E. Murphy-Ullrich (2012) Matrix Biol. 31:178.
  11. Grimbert, P. et al. (2006) J. Immunol. 177:3534.
  12. Kaur, S. et al. (2011) J. Biol. Chem. 286:14991.
  13. Xu, J. et al. (2010) Nat. Neurosci. 13:22.
  14. Eroglu, C. et al. (2009) Cell 139:380.
  15. Blake, S.M. et al. (2008) EMBO J. 27:3069.
  16. Moodley, Y. et al. (2003) Am. J. Pathol. 162:771.

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Publications for Thrombospondin-1 (3074-TH)(20)

We have publications tested in 3 confirmed species: Human, Mouse, N/A.

We have publications tested in 4 applications: Bioassay, ELISA (Standard), Enzyme Assay, In Vivo.


Filter By Application
Bioassay
(16)
ELISA (Standard)
(2)
Enzyme Assay
(1)
In Vivo
(1)
All Applications
Filter By Species
Human
(16)
Mouse
(3)
N/A
(1)
All Species
Showing Publications 1 - 10 of 20. Show All 20 Publications.
Publications using 3074-TH Applications Species
S Lie, BR Vieira, S Arruda, M Simões, LM Ashander, JM Furtado, JR Smith Molecular Basis of The Retinal Pigment Epithelial Changes That Characterize The Ocular Lesion in Toxoplasmosis Microorganisms, 2019;7(10):. 2019 [PMID: 31569536] (Bioassay, Human) Bioassay Human
J Guillon, C Petit, M Moreau, B Toutain, C Henry, H Roché, N Bonichon-L, JP Salmon, J Lemonnier, M Campone, V Verrièle, E Lelièvre, C Guette, O Coqueret Regulation of senescence escape by TSP1 and CD47 following chemotherapy treatment Cell Death Dis, 2019;10(3):199. 2019 [PMID: 30814491] (Bioassay, Human) Bioassay Human
A Ojha, A Bhasym, S Mukherjee, GK Annarapu, T Bhakuni, I Akbar, T Seth, NK Vikram, S Vrati, A Basu, S Bhattachar, P Guchhait Platelet factor 4 promotes rapid replication and propagation of Dengue and Japanese encephalitis viruses EBioMedicine, 2019;39(0):332-347. 2019 [PMID: 30527622] (Bioassay, Human) Bioassay Human
G Inana, C Murat, W An, X Yao, IR Harris, J Cao RPE phagocytic function declines in age-related macular degeneration and is rescued by human umbilical tissue derived cells J Transl Med, 2018;16(1):63. 2018 [PMID: 29534722] (Bioassay, Human) Bioassay Human
Dong Hyun Kim, Hoon Lim, Dahm Lee, Soo Jin Choi, Wonil Oh, Yoon Sun Yang, Jeong Su Oh, Hyun Ho Hwang, Hong Bae Jeon Thrombospondin-1 secreted by human umbilical cord blood-derived mesenchymal stem cells rescues neurons from synaptic dysfunction in Alzheimer&#039;s disease model. Scientific Reports, 2018;0(0):2045-2322. 2018 [PMID: 29321508] (Bioassay, Mouse) Bioassay Mouse
R Bibes, S Gobron, F Vincent, C Mélin, N Vedrenne, A Perraud, F Labrousse, MO Jauberteau, F Lalloué SCO-spondin oligopeptide inhibits angiogenesis in glioblastoma Oncotarget, 2017;8(49):85969-85983. 2017 [PMID: 29156770] (Bioassay, Human) Bioassay Human
K Endt, J Goepfert, A Omlin, A Athanasiou, P Tennstedt, A Guenther, M Rainisio, DS Engeler, T Steuber, S Gillessen, T Joos, R Schiess Development and clinical testing of individual immunoassays for the quantification of serum glycoproteins to diagnose prostate cancer PLoS ONE, 2017;12(8):e0181557. 2017 [PMID: 28767721] (ELISA (Standard), Human) ELISA (Standard) Human
C Sallon, I Callebaut, I Boulay, J Fontaine, D Logeart-Av, C Henriquet, M Pugnière, X Cayla, P Monget, G Harichaux, V Labas, S Canepa, C Taragnat Thrombospondin-1 (TSP-1), a new bone morphogenetic protein-2 and -4 (BMP-2/4) antagonist identified in pituitary cells J. Biol. Chem., 2017;0(0):. 2017 [PMID: 28747434] (Bioassay, Human) Bioassay Human
J Zhai, J Liu, X Cheng, S Li, Y Hong, K Sun, ZJ Chen, Y Du, W Li Zinc finger gene 217 (ZNF217) Promoted Ovarian Hyperstimulation Syndrome (OHSS) through Regulating E2 Synthesis and Inhibiting Thrombospondin-1 (TSP-1) Sci Rep, 2017;7(1):3245. 2017 [PMID: 28607476] (Bioassay, Human) Bioassay Human
A Torres, MA Gubbiotti, RV Iozzo Decorin-inducible Peg3 Evokes Beclin 1-mediated Autophagy and Thrombospondin 1-mediated Angiostasis J. Biol. Chem, 2017;292(12):5055-5069. 2017 [PMID: 28174297] (Bioassay, Human) Bioassay Human
Show All 20 Publications.

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Bioinformatics

Gene Symbol THBS1
Uniprot