Recombinant Human TEM5/GPR124 His-tag Protein, CF

When Recombinant Human RECK Fc Chimera (Catalog # 10309-RE) is immobilized at 2 μg/mL (100 μL/well), Recombinant Human TEM5/GPR124 His-tag (Catalog # 10206-TE) binds with an ED50 of 1‑8 μg/mL.

2 μg/lane of Recombinant Human TEM5/GPR124 His-tag (Catalog # 10206-TE) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 53-60 kDa.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human TEM5/GPR124 His-tag Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human RECK Fc Chimera (Catalog # 10309-RE) is immobilized at 2 µg/mL (100 µL/well), Recombinant Human TEM5/GPR124 His-tag (Catalog # 10206-TE) binds with an ED₅₀ of 1‑8 μg/mL.
• Source: Human embryonic kidney cell, HEK293-derived human TEM5/GPR124 protein
  Ala27-Arg359, with C-terminal 6-His tag
• Accession #: AAO27354
• N-terminal Sequence: Ala27
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 36 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 53-60 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human TEM5/GPR124 His-tag Protein, CF

• DKFZp434C211
• DKFZp434J0911
• G protein-coupled receptor 124
• GPR124
• G-protein coupled receptor 124
• KIAA1531
• KIAA1531FLJ14390
• TEM5
• TEM5Tumor endothelial marker 5

Background

G-protein coupled receptor 124 (GPR124), otherwise known as Adhesion G protein-coupled receptor A2 (AGRA2) and tumor endothelial marker 5 (TEM5), is localized on the surface of endothelial cells. Adhesion GPCRs are the second largest group within the GPCR superfamily, and are implicated in cell-to-cell and cell-to-matrix adhesion with high promiscuity towards ligands (1, 2). Humam TEM5/GPR124 contains a 738 amino acid (aa) extracellular domain (ECD), a transmembrane domain with seven helices of varying lengths, and a 270 aa cytoplasmic domain. Human TEM5/GPR124 shares 90% aa identity to both mouse and rat TEM5/GPR124 within the  N-terminal ECD. TEM5/GPR124 shares structural characteristics with other adhesion GPCR family members because of its relatively large N and C-terminal domains, and a GAIN domain (1, 2). The N-terminal ECD contains an exposed RGD motif and binds to RGD-dependent integrins on the surface of endothelial cells. TEM5/GPR124 mediates endothelial cell survival during angiogenesis by linking integrin to glycosaminoglycans. TEM5/GPR124 has also been shown to interact with DLG1 through its PDZ-binding motif (3). In humans, TEM5/GPR124 appears to play a role in tumor vascular biology, as TEM5/GPR124 transcripts are dramatically up-regulated in vascular ECs in tumors relative to ECs in normal tissue (4). The most N-terminal domain of RECK binds to the leucine-rich repeat (LRR) and immunoglobulin (Ig) domains of TEM5/GPR124 (4). RECK is the predominant binding partner of TEM5/GPR124 (5). TEM5/GPR124 and RECK act primarily in an extracellular fashion. RECK has been identified as a TEM5/GPR124-associated Wnt7 receptor that forms a 1:1 complex with active, monomeric, hydrophobic Wnt7 (5). RECK and TEM5/GPR124 are part of the cell surface protein complex that transduces Wnt7a- and Wnt7b-specific signals in mammalian CNS ECs to promote angiogenesis and regulate the blood-brain barrier (BBB) (4).

1. Hamann, J. et al. (2015) Pharmacol Rev. 67:338.
2. Arac, D. et al. (2012) Ann N Y Acad Sci. 1276:1.
3. Kuhnert, F. et al. (2010) Science 330:985.
4. Cho, C. et al. (2017) Neuron. 95:1056.
5. Vallon, M. et al. (2018) Cell Reports 25:339.

Bioinformatics

• Uniprot:
  • AAO27354()