Recombinant Human ST6GALNAC4 Protein, CF


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Product Details

Reactivity HuSpecies Glossary
Applications Enzyme Activity

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Recombinant Human ST6GALNAC4 Protein, CF Summary

Details of Functionality
Measured by its ability to transfer Neu5Ac from CMP-Neu5Ac to fetuin of fetal calf serum. The specific activity is >1000 pmol/min/μg, as measured under the described conditions.
Mouse myeloma cell line, NS0-derived human ST6GALNAC4 protein
Thr36-Arg302, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Thr36 and Leu38
Protein/Peptide Type
Recombinant Enzymes
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.


Theoretical MW
31 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
36-38 kDa, reducing conditions

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Supplied as a 0.2 μm filtered solution in Tris and NaCl.
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.
Assay Procedure
  • Assay Buffer: 50 mM Tris, 10 mM MnCl2 (supplied in kit), pH 7.0
  • Recombinant Human ST6GALNAC4 (rhST6GALNAC4) (Catalog # 6876-GT)
  • Donor Substrate: CMP-Sialic Acid (Sigma, Catalog # C8271), 10 mM stock in deionized water
  • Acceptor Substrate: Fetuin (Sigma, Catalog # F3385), 50 mg/mL stock in diH20
  • Sialyltransferase Activity Kit (Catalog # EA002)
  • 96-well Clear Plate (Costar, Catalog # 92592)
  • Plate Reader (Model: SpectraMax Plus by Molecular Devices) or equivalent
  1. Dilute CMP-Sialic Acid to 2.5 mM in Assay Buffer.
  2. Dilute Coupling Phosphatase 2 to 10 µg/mL in Assay Buffer.
  3. Prepare reaction mixture by combining 75 µL of 2.5 mM CMP-Sialic Acid, 150 µL of 10 µg/mL Coupling Phosphatase 2, 75 µL of 50 mg/mL Fetuin, and 75 µL of Assay Buffer.
  4. Dilute rhST6GALNAC4 to 0.8 µg/mL in Assay Buffer.
  5. Dilute 1 mM Phosphate Standard by adding 40 µL to 360 µL of Assay Buffer for a 100 μM stock. This is the first point of the standard curve.
  6. Continue standard curve by performing six one-half serial dilutions of the 100 µM Phosphate stock in Assay Buffer. The standard curve has a range of 0.078 to 5.0 nmol per well.
  7. Load 50 µL of each dilution of the standard curve into a plate. Include a curve blank containing 50 μL of Assay Buffer.
  8. Load 25 µL of the 0.8 µg/mL rhST6GALNAC4 into the plate. Include a control containing 25 µL of Assay Buffer.
  9. Add 25 µL of reaction mixture to the wells, excluding the standard curve.
  10. Cover the plate with parafilm or a plate sealer and incubate at 37 ºC for 20 minutes.
  11. Add 30 µL of the Malachite Green Reagent A to all wells.  Mix and incubate for 10 minutes at room temperature.
  12. Add 100 µL of deionized water to all wells. Mix briefly.
  13. Add 30 µL of the Malachite Green Reagent B to all wells. Mix and incubate for 20 minutes at room temperature.
  14. Read plate at 620 nm (absorbance) in endpoint mode.
  15. Calculate specific activity:

     Specific Activity (pmol/min/µg) =

Phosphate released* (nmol) x (1000 pmol/nmol)
Incubation time (min) x amount of enzyme (µg)

     *Derived from the phosphate standard curve using linear or 4-parameter fitting and adjusted for the control.

Per Reaction:
  • rhST6GALNAC4: 0.02 µg
  • Coupling Phosphatase 2: 0.1 µg
  • Fetuin: 250 µg
  • CMP-Sialic Acid: 0.25 mM


Coomassie is a registered trademark of Imperial Chemical Industries Ltd.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human ST6GALNAC4 Protein, CF

  • EC 2.4.99
  • EC
  • NeuAc-alpha-2,3-Gal-beta-1,3-GalNAc-alpha-2,6-sialyltransferase IV
  • NeuAc-alpha-2,3-Gal-beta-1,3-GalNAc-alpha-2,6-sialyltransferase
  • Sialyltransferase 3C
  • Sialyltransferase 7D
  • sialyltransferase 7D((alpha-N-acetylneuraminyl-2,3-beta-galactosyl-1,3)-N-acetyl galactosaminidealpha-2,6-sialyltransferase)
  • SIAT3C
  • SIAT3-C
  • SIAT3C6-sialyltransferasealpha2,6-sialyltransferase
  • SIAT7D
  • SIAT7-D
  • ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminidealpha-2,6-sialyltransferase 4
  • ST6GalNAc IV
  • ST6GalNAcIV


ST6GALNAC4 is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates (1). This enzyme works on both glycoproteins and glycolipids and has strict substrate specificity, utilizing only the trisaccharide sequence Neu5Ac alpha 2-3Gal beta 1-3GalNAc of glycoproteins and glycolipids (2). In particular, this enzyme is involved in the synthesis of ganglioside GD1A from GM1B (3). GD1A is the target pathogenic antigen in the autoimmune disease, Guillain-Barre Syndrome (4, 5). The activity of this enzyme has been measured using a phosphatase-coupled method (6).
  1. Harduin-Lepers, A. et al. (2005) Glycobiology 15:805.
  2. Harduin-Lepers, A. et al. (2000) Biochem. J. 352:37.
  3. Lee, Y. C. et al. (1999) J. Biol. Chem. 274:11958.
  4. Kusunoki, S. et al. (1994) Ann. Neurol. 35:570.
  5. Goodfellow, J.A. et al. (2005) J. Neurosci. 25:1620.
  6. Wu, Z.L. et al. (2011) Glycobiology 21:727.

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Gene Symbol ST6GALNAC4