Recombinant Human SCF Protein, CF

Recombinant Human SCF/c‑kit Ligand Protein (Catalog # 11010-SC) induces proliferation of TF‑1 human erythroleukemic cells. The ED50 for this effect is 1.00‑5.00 ng/mL.

2 μg/lane of Recombinant Human SCF/c‑kit Ligand Protein (Catalog # 11010-SC) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 22-32 kDa.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human SCF Protein, CF Summary

• Additional Information: HEK293-expressed
• Details of Functionality: Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED₅₀ for this effect is 1.00-5.00 ng/mL.
• Source: Human embryonic kidney cell, HEK293-derived human SCF/c-kit Ligand protein
  Glu26-Ala189
• Accession #: P21583.1
• N-terminal Sequence: Glu26
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 18 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 22-31 kDa, under reducing conditions.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 100-500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human SCF Protein, CF

• c-kit Ligand
• DCUA
• DFNA69
• DKFZp686F2250
• familial progressive hyperpigmentation 2
• FPH2
• FPHH
• KIT ligand
• Kitl
• KITLG
• KL-1
• Mast cell growth factor
• MGF
• MGFSHEP7
• SCF
• SCFStem cell factor
• SFc-Kit ligand
• SHEP7
• SLF
• steel factor

Background

Stem cell factor (SCF) is a potent hematopoietic growth factor required in regulating both embryonic and adult hematopoiesis. SCF protein promotes the survival, differentiation, and mobilization of multiple cell types including myeloid, erythroid, megakaryocytic, lymphoid, germ cell, and melanocyte progenitors (1 7). SCF is a primary growth and activation factor for mast cells and eosinophils (8). And SCF assists in the recovery of cardiac function following myocardial infarction by increasing the number of cardiomyocytes and vascular channels (9). Stem cell factor is an important cytokine for ex vivo clinical applications. Along with other cytokines, SCF is used in the culture and expansion of hematopoietic stem cells (HSCs) as well as for proliferation and differentiation of both myeloid and erythroid progenitor cells. 

Mature stem cell factor consists of a 189 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane domain, and a 36 aa cytoplasmic tail (10). The ECD shows both N linked and O-linked glycosylation (11). SCF protein exists in two forms, a membrane-bound form and a proteolytically processed soluble form that lacks the transmembrane domain and cytoplasmic tail. The soluble form is created by proteolytic cleavage at two alternate sites in the extracellular juxtamembrane region releasing a 25 kDa soluble SCF protein which is comparable to the only form produced by Steel-dickie mutant mice (12, 13). There is also an alternately spliced isoform of human SCF that lacks 28 amino acids that encompasses the primary proteolytic recognition site (14). This form cannot be cleaved and is only membrane bound. SCF binds to C-kit (CD117). C-kit is expressed on many different cell types including HSCs, mast cells, germ cells, and melanocytes. Binding of SCF to C-kit induces receptor dimerization and autophosphorylation of tyrosine residues in the cytoplasmic domain (15). Tyrosine phosphorylation initiates multiple signaling pathways including RAS, PI3 kinase, Src, and JAK/STAT. Stem cell factor is highly conserved among mammals. Human SCF protein shares 79% 87% aa sequence identity with dog, cat, mouse, and rat SCF. Rat SCF is active on mouse and human cells, but human SCF is only weakly active on mouse cells (10). 

SCF is a versatile factor in the differentiation of many specific cell types like spermatogonial stem cells (16) and megakaryocyte progenitors (17). Apart from differentiation, SCF also can maintain stemness in cells. This is the case for human bone marrow mesenchymal cells, which require SCF and hepatocyte growth factor for maintenance (18). Hematopoietic stem cells similarly require SCF from surrounding cells in their niche to maintain their stemness and their progenitors (19). SCF has also improved protocols for continuous generation of cells in culture systems, like granulocytes and macrophages (20).  

For treatment of graft versus host disease, SCF is used in combination with other cytokines to generate myeloid-derived suppressor cells from human umbilical cord blood (21). SCF is also used to generate T cells for cell-based therapies, drug screening and disease modeling (22). In regenerative studies, SCF is applied in wound healing hydrogel as a means of increasing its adhesion strength and tissue regeneration (23).

1. Ashman, L.K. (1999) Int. J. Biochem. Cell Biol. 31:1037. 
2. Sette, C. et al. (2000) Int. J. Dev. Biol. 44:599. 
3. Yoshida, H. et al. (2001) J. Invest. Dermatol. Symp. Proc. 6:1. 
4. Erlandsson, A. et al. (2004) Exp. Cell Res. 301:201. 
5. Kapur, R. et al. (2002) Blood 100:1287. 
6. Wang, C.H. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:540. 
7. Bashamboo, A. et al. (2006) J. Cell Sci. 119:3039. 
8. Reber, L. et al. (2006) Eur. J. Pharmacol. 533:327.
9. Kanellakis, P. et al. (2006) Cardiovasc. Res. 70:117.
10. Martin, F.H. et al. (1990) Cell 63:203.
11. Arakawa, T. et al. (1991) J. Biol. Chem. 266:18942.
12. Majumdar, M.K. et al. (1994) J. Biol. Chem. 269:1237.
13. Brannan, C.I. et al. (1991) Proc. Natl. Acad. Sci. 88:4671.
14. Anderson, D.M. et al. (1991) Cell Growth Differ. 2:373.
15. Lemmon, M.A. et al. (1997) J. Biol. Chem. 272:6311.
16. Nasimi, M. et al. (2021) Reprod Sci. 28:963.
17. Krisch, L. et al. (2021) Int. J. Mol. Sci. 22:8224.
18. Cao, Z. et al. (2020) Stem Cell Res Ther. 11:1.
19. Comazzetto, S. et al. (2019) Cell Stem Cell. 24:477.
20. Bernecker, C. et al. (2019) Stem Cells Dev. 28:1540.
21. Park, M.Y. et al. (2019) Front Immunol. 10:1.
22. Netsrithong, R. et al. (2020) Stem Cell Res Ther. 11:1.
23. Zhang, Li. et al. (2021) Journal Mater Chem B. 29:5887.

Publications for SCF/c-kit Ligand (11010-SC)(2)

Publications using 11010-SC

• Pickering, S;Wilson, H;Bravo, E;Perera, MR;Seow, J;Graham, C;Almeida, N;Fotopoulos, L;Williams, T;Moitra, A;Winstone, H;Nissen, TAD;Galão, RP;Snell, LB;Doores, KJ;Malim, MH;Neil, SJD; Antibodies to the RBD of SARS-CoV-2 spike mediate productive infection of primary human macrophages Nature communications 2024-12-30 [PMID: 39737903] (Bioassay, Human)
• Arend W. Overeem, Yolanda W. Chang, Ioannis Moustakas, Celine M. Roelse, Sanne Hillenius, Talia Van Der Helm et al. Efficient and scalable generation of primordial germ cells in 2D culture using basement membrane extract overlay Cell Reports Methods 2023-06-26 [PMID: 37426764] (Bioassay, Human)

Bioinformatics

• Uniprot:
  • P21583.1()