Recombinant Human SALM4/LRFN3 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human SALM4/LRFN3 Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of Neuro‑2A mouse neuroblastoma cells. When 5 x 10⁴ cells/well are added to rhSALM4 coated plates (2.5 μg/mL, 100 μL/well), approximately 50%-70% will adhere after 30 minutes at 37 °C. Optimal dilutions should be determined by each laboratory for each application.
• Source: Mouse myeloma cell line, NS0-derived human SALM4/LRFN3 protein
  Ser17-Met539 with a C-terminal 6-His tag
• Accession #: Q9BTN0
• N-terminal Sequence: Ser17
• Protein/Peptide Type: Recombinant Proteins
• Gene: LRFN3
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 56.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 80-85 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 200 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human SALM4/LRFN3 Protein, CF

• fibronectin type III, immunoglobulin and leucine rich repeat domains 1
• FIGLER1
• leucine rich repeat and fibronectin type III domain containing 3
• leucine-rich repeat and fibronectin type-III domain-containing protein 3
• LRFN3
• MGC2656
• SALM4
• synaptic adhesion-like molecule 4
• UNQ5865/PRO34192

Background

Synaptic adhesion-like molecule 4 (SALM4; also leucine-rich repeat and fibronectin type-III domain-containing protein 3 (Lrfn3) is an approximately 90 kDa member of the Lrfn family of type I transmembrane glycoproteins (1).  Human SALM4 is synthesized as a 628 amino acid (aa) precursor that contains a 16 aa signal sequence, a 523 aa extracellular domain (ECD), a 21 aa transmembrane region, and a 68 aa cytoplasmic region.  The ECD consists of six leucine-rich repeats (LRR), an IgC2-like domain, and a fibronectin type-III domain, tandemly aligned in that order (1 - 2).  In addition, there are five potential sites for N-linked glycosylation.  SALM4 and -5 lack a C-terminal intracellular PDZ binding domain, which is conserved among SALMs 1-3.  Mature human SALM4 shares 96% aa sequence identity with mature mouse SALM4.  Northern blot analysis showed that in mice, SALM4 is strongly expressed in the adult brain and is also present in the adult gastrointestinal tract and kidneys (1). It is distributed throughout the neuron, including the growth cone (3).  In the developing mouse embryo, a temporal expression profile blot revealed a general increment of expression around E10.5, with weak expression detected before E10.5 (1). SALM4, like the other SALMs, promotes neurite outgrowth (3).  Specifically, the SALMs modify total outgrowth and neurite branching (3).

1. Morimura, N. et al. (2006) Gene 380:72.
2. Wang, C.-Y. et al. (2006) J. Neurosci. 26:2174.
3. Wang, P.Y. et al. (2008) Mol. Cell. Neurosci. 39:83.

Bioinformatics

• Gene Symbol: LRFN3
• Uniprot:
  • Q9BTN0()