SALM2/LRFN1 Antibody [Unconjugated]

Western blot shows lysates of human brain cortex and hipoocampus tissue and negative control lung and liver tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human SALM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5669) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for SALM2 at approximately 90-110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

SALM2/LRFN1 Antibody [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human SALM2/LRFN1
• Specificity: Detects human SALM2/LRFN1 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) SALM3 and rhSALM4 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: LRFN1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SALM2/LRFN1 Antibody [Unconjugated]

• KIAA1484
• leucine rich repeat and fibronectin type III domain containing 1
• leucine-rich repeat and fibronectin type III domain-containing protein 1
• LRFN1
• SALM2
• synaptic adhesion-like molecule 2

Background

Synaptic adhesion-like molecule 2 (SALM2; also leucine-rich repeat and fibronectin type-III domain-containing protein 1 (Lrfn1) is a 105 kDa member of the Lrfn family of type I transmembrane glycoproteins (1‑2). Human SALM2 is synthesized as a 771 amino acid (aa) precursor that contains a 31 aa signal sequence, a 505 aa extracellular domain (ECD), a 21 aa transmembrane region, and a 214 aa cytoplasmic region.  The ECD consists of seven leucine-rich repeats (LRR), an IgC2-like domain, and a fibronectin type-III domain, tandemly aligned in that order (1, 3). In addition, there are two potential sites for N-linked glycosylation. The C-terminal region contains an intracellular PDZ binding domain, which is conserved among SALMs 1‑3, but is absent in SALMs 4 and 5 (4). Mature human SALM2 shares 96% and 95% aa sequence identity with mature mouse and rat SALM2, respectively. In the developing mouse embryo, the temporal expression profile blot revealed a general increment of expression around E10.5 for SALM2. Northern blot analysis showed that in rats, SALM2 is strongly expressed in the adult brain and is also present in the adult testis (2). SALM2 localizes to both axons and dendrites (4). In addition, it co-localizes with both pre- and post-synaptic proteins at excitatory synapses in mature neurons (2, 4). SALM2, like the other SALMs, promotes neurite outgrowth (4). In particular, SALM2 promotes an increase in the number of primary processes compared to controls and SALMs 1 and 5 (4). In addition, SALM2 is an important regulator of the differentiation of excitatory synapses (2). SALM2 may also be involved in synaptic maintenance and other cellular interactions (4).

1. Morimura, N. et al. (2006) Gene 380:72.
2. Ko, J. et al. (2006) Neuron 50:233.
3. Wang, C.-Y. et al. (2006) J. Neurosci. 26:2174.
4. Wang, P.Y. et al. (2008) Mol. Cell. Neurosci. 39:83.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SALM2/LRFN1 Antibody (AF5669)(1)

Publication using AF5669

• N Apóstolo, SN Smukowski, J Vanderlind, G Condomitti, V Rybakin, J Ten Bos, L Trobiani, S Portegies, KM Vennekens, NV Gounko, D Comoletti, KD Wierda, JN Savas, J de Wit Synapse type-specific proteomic dissection identifies IgSF8 as a hippocampal CA3 microcircuit organizer Nat Commun, 2020-10-14;11(1):5171. 2020-10-14 [PMID: 33057002] (IHC, Mouse)

Bioinformatics

• Gene Symbol: LRFN1