Recombinant Human S100A4 Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human S100A4 Protein, CF Summary

Details of Functionality
Measured by its ability to enhance neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons. Able to significantly enhance neurite outgrowth when immobilized as a 3 µL droplet containing 90 ng on a nitrocellulose-coated microplate.
Source
E. coli-derived human S100A4 protein
Ala2-Lys101, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Ala2
Protein/Peptide Type
Recombinant Proteins
Gene
S100A4
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
12.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
4137-S4 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS and DTT.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human S100A4 Protein, CF

  • 18A2
  • 42A
  • Calvasculin
  • CAPL
  • CAPLS100 calcium binding protein A4 (calcium protein, calvasculin, metastasin
  • fibroblast-specific protein-1,42A
  • FSP1
  • leukemia multidrug resistance associated protein
  • malignant transformation suppression 1
  • Metastasin
  • MTS1
  • murine placental homolog)
  • P9KA
  • PEL98
  • Placental calcium-binding protein
  • Protein Mts1
  • protein S100-A4
  • S100 calcium binding protein A4
  • S100 calcium-binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog)
  • S100 calcium-binding protein A4
  • S100A4

Background

S100A4 (also metastasin, Mtsl and calvasculin) is an 11 kDa member of the S100 (soluble in 100% saturated ammonium sulfate) family of proteins (1 - 5). The S100 family is further classified as a member of the EF-hand superfamily of Ca++-binding proteins. These participate in both calcium-dependent and calcium-independent protein-protein interactions. The hallmark of this superfamily is the EF-hand motif that consists of a Ca++-binding site flanked by two alpha -helices (helix E and helix F) that were originally identified in a right-handed model of carp muscle calcium-binding protein (6). Human S100A4 is 101 amino acids (aa) in length (1, 2). It contains two EF hand domains, one between aa 12 - 47, and a second between aa 50 - 85. The first domain has a 14 aa cation-binding motif and binds Ca++ with low affinity. The second Ca++-binding motif is 12 aa in length and binds Ca++ with high affinity. S100A4 has no classical signal sequence but is secreted from cells (3, 7). Human S100A4 shares 93% and 95% aa identity with mouse and canine S100A4, respectively. S100A4 reportedly exists as a dimer (8, 9, 10). Extracellular S100A4 is reported to induce MMP production, activate MMPs, promote neurite outgrowth and stimulate cardiomyocyte proliferation (4, 10, 11, 12, 13). Within the cell, dimers are likely the functional unit. Here, they are constitutive homo- or heterodimers (with S100A1) that interact with Ca++, undergo a conformational change, and subsequently bind to cytoplasmic targets. Known targets include p53, myosin heavy chain II, F-actin and liprin beta 1 (4, 14). In general, it can be said that S100A4 blocks target phosphorylation and multimerization (4, 7, 14). S100A4 activity has been associated with cell transformation. It seems likely this is either coincidental, or a consequence, rather than a cause of transformation (3).

  1. Engelkamp, D. et al. (1992) Biochemistry 31:10258.
  2. Tomida, Y. et al. (1992) Biochem. Biophys. Res. Commun. 189:1310.
  3. Garrett, S.C. et al. (2006) J. Biol. Chem. 281:677.
  4. Santamaria-Kisiel, L. et al. (2006) Biochem. J. 396:201.
  5. Donato, R. (2001) Int. J. Biochem. Mol. Biol. 33:637.
  6. Kretsinger, R.H. and C.E. Nockolds (1973) J. Biol. Chem. 248:3313.
  7. Helfman, D.M. et al. (2005) Br. J. Cancer 92:1955.
  8. Burkitt, W.I. et al. (2003) Biochem. Soc. Trans. 31:985.
  9. Vallaly, K.M. et al. (2002) Biochemistry 41:12670.
  10. Novitskaya, V. et al. (2000) J. Biol. Chem. 275:41278.
  11. Stary, M. et al. (2006) Biochem. Biophys. Res. Commun. 343:555.
  12. Semov, A. et al. (2005) J. Biol. Chem. 280:20833.
  13. Saleem, M. et al. (2006) Proc. Natl. Acad. Sci. USA 103:14825.
  14. Kriajevska, M. et al. (2002) J. Biol. Chem. 277:5229.

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Publications for S100A4 (4137-S4)(4)

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Bioinformatics

Gene Symbol S100A4
Uniprot