Recombinant Human Ryk Fc Chimera Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Ryk Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its ability to bind biotinylated recombinant mouse Wnt-3a in a functional ELISA with an estimated K_D <50 nM.
• Source: Chinese Hamster Ovary cell line, CHO-derived human Ryk protein
  

  Human Ryk (Ala46-Thr224) Accession # NP_001005861	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: NP_001005861
• N-terminal Sequence: Ala46
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: RYK
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 46.7 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 60-70 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Ryk Fc Chimera Protein, CF

• D3S3195
• EC 2.7.10
• EC 2.7.10.1
• hydroxyaryl-protein kinase
• JTK5
• JTK5A protein tyrosine kinase
• JTK5A
• RYK receptor-like tyrosine kinase
• Ryk
• RYK1
• tyrosine-protein kinase RYK

Background

Ryk (Related to tyrosine kinase) is an 70-90 kDa type I transmembrane glycoprotein that is an atypical member of the receptor tyrosine kinase superfamily (1, 2). Human Ryk cDNA encodes 604 amino acids (aa) that include a 25 aa signal sequence, a 199 aa extracellular domain (ECD, aa 26‑224) that contains a WIF (Wnt Inhibitory Factor) domain (aa 63‑191), a transmembrane sequence, and a cytoplasmic region with a nonfunctional Ser/Thr protein kinase domain (aa 327‑600) (1‑3). The cytoplasmic region participates in signaling by undergoing gamma ‑secretase cleavage and translocating to the nucleus in response to Wnts (4). An isoform with a 31 aa substitution between aa 18‑46 is reported. Within the ECD, human Ryk shares approximately 94%, 92%, 86% and 81% aa sequence identity with mouse, rat, equine and canine Ryk, respectively. Ryk protein expression is reported in developing neurons of the corticospinal tract, ventral zone, and retina, as well as adult tissue epithelia, stroma and blood vessels (4‑9). Ryk binds Wnts and EphB2/B3 receptors via its WIF domain (8‑10). It may form a Wnt receptor complex with Frizzled receptors, serving as a link between Wnt and Dishevelled and converting Wnt/Frizzled attraction signals to repulsion signals (1, 6‑8, 10). In particular, Ryk is required for Wnt5a‑induced axon guidance after cortical axons cross the corpus callosum, and for Wnt3-mediated guidance of developing retinal ganglion cells (7, 8). Mice deleted for the Ryk gene show defects in axon guidance that produce craniofacial defects and shortened limbs (10, 11). Ryk^-/- mice share a cleft palate phenotype with EphB2/B3-deleted mice (10). Ryk is often overexpressed in ovarian cancer (5).

1. Stacker, S.A. et al. (1993) Oncogene 8:1347.
2. Clark, C.E.J. et al. (2012) Neurosignals 20:202.
3. Katso, R.M. et al. (1999) Mol. Cell. Biol. 19:6427.
4. Lyu, J. et al. (2008) Dev. Cell 15:773.
5. Katso, R.M. et al. (1999) Cancer Res. 59:2265.
6. Liu, Y. et al. (2005) Nat. Neurosci. 8:1151.
7. Schmitt, A.M. et al. (2006) Nature 439:31.
8. Keeble, T.R. et al. (2006) J. Neurosci. 26:5840.
9. Kamitori, K. et al. (2005) Biochem. Biophys. Res. Commun. 330:446.
10. Lu, W. et al. (2004) Cell 119:97.
11. Halford, M.M. et al. (2000) Nat. Genet. 25:414.

Bioinformatics

• Gene Symbol: RYK
• Uniprot:
  • NP_001005861()