Recombinant Human Ret His-tag Protein, CF

When Recombinant Human Ret His-tag (Catalog # 1168-CR) is immobilized at 1 μg/mL (100 μL/well), Recombinant Human GDNF (Catalog # 212-GD) binds with an ED50 of 0.05-0.3 μg/mL in the presence of Recombinant Human GFR alpha-1/GDNF R alpha-1 Fc Chimera (Catalog # 714-GR).

Product Discontinued

Recombinant Human Ret His-tag Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA.

  When Recombinant Human Ret His-tag is immobilized at 1 µg/mL (100 µL/well), Recombinant Human GDNF (Catalog # 212-GD) binds with an ED₅₀ of 0.05-0.3 μg/mL in the presence of Recombinant Human GFR alpha ‑1/GDNF R alpha‑1 Fc Chimera (Catalog # 714-GR).

• Source: Spodoptera frugiperda, Sf 21 (stably transfected)-derived human Ret protein
  Leu29-Arg635, with a C-terminal 10-His tag
• Accession #: P07949
• N-terminal Sequence: Leu29
• Protein/Peptide Type: Recombinant Proteins
• Gene: RET
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 69 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 90 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Ret His-tag Protein, CF

• CDHF12MTC1
• CDHR16
• EC 2.7.10
• Hirschsprung disease 1
• Hirschsprung disease)
• MEN2A
• MEN2B
• multiple endocrine neoplasia and medullary thyroid carcinoma 1
• ret proto-oncogene
• RET transforming sequence
• Ret

Background

The GDNF family of neurotrophic factors forms a subfamily within the TGF-beta superfamily. These proteins are potent survival factors for various central and peripheral neurons during development and the adult animal. The GDNF family members (GDNF, neurturin, artemin and persephin) signal through multicomponent receptors that consist of the Ret receptor tyrosine kinase and one of four glycosyl-phosphatidylinositol (GPI)-linked ligand-binding subunits (GFR alpha -1 - 4). GFR alpha -1 -2, -3 and -4 are the preferred ligand-binding subunits for GDNF, neurturin, artemin and persephin, respectively. The Ret tyrosine-kinase receptor is encoded by the c-ret proto-oncogene. Mutations of the ret gene have been associated with various human diseases affecting tissues derived from the neural crest, including Hirschsprung’s disease, multiple endocrine neoplasia MEN2A and MEN2B, and familial medullary thyroid carcinoma. Human and mouse Ret share 83% amino acid sequence homology (77% homology in the extracellular domain and 93% homology in the cytoplasmic domain). Although Ret does not bind GDNF ligands directly, the extracellular domain of Ret binds the GDNF-GFR-alpha complex with high affinity and is a potent GDNF antagonist in the presence of soluble GFR-alpha (1-4).

1. Trupp, M. et al. (1998) Mol. Cell. Neurosci. 11:47.
2. Enokido, Y. et al. (1998) Curr. Biol. 8:1019.
3. Carlomagno, F. et al. (1998) Endocrinology 139:3613.
4. Baloh, R. et al. (1998) Neuron 21:1291.

Publications for Ret (1168-CR/CF)(2)

Publications using 1168-CR/CF

• Chu Y, Bartus R, Manfredsson F, Olanow C, Kordower J Long-term post-mortem studies following neurturin gene therapy in patients with advanced Parkinson&#039;s disease. Brain, 2020-03-01;143(3):960-975. 2020-03-01 [PMID: 32203581] (Adsorption, Mouse)
• Lu B, PereiraPerrin M A novel immunoprecipitation strategy identifies a unique functional mimic of the glial cell line-derived neurotrophic factor family ligands in the pathogen Trypanosoma cruzi. Infect. Immun., 2008-06-09;76(8):3530-8. 2008-06-09 [PMID: 18541656] (PcIP, Bacteria)

Bioinformatics

• Gene Symbol: RET
• Uniprot:
  • P07949()