Recombinant Human R-Spondin 4 Protein, CF


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Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human R-Spondin 4 Protein, CF Summary

Details of Functionality
Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 10‑60 ng/mL in the presence of 10 ng/mL Recombinant Mouse Wnt‑3a (Catalog # 1324-WN).
Chinese Hamster Ovary cell line, CHO-derived human R-Spondin 4 protein
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.


Theoretical MW
24.1 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
30-36 kDa, reducing conditions
Read Publications using
4575-RS/CF in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS.
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 200 μg/mL in PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human R-Spondin 4 Protein, CF

  • C20orf182
  • chromosome 20 open reading frame 182
  • Cristin 4
  • dJ824F16.3
  • FLJ16018
  • hRspo4
  • Roof plate-specific spondin-4
  • RSPO4
  • RSpondin 4
  • R-Spondin 4
  • R-spondin family, member 4
  • R-spondin-4


R-Spondin 4 (RSPO4, roof plate-specific spondin 4), also called cysteine‑rich and single thrombospondin domain containing-4 (Cristin 4), is an ~33 kDa secreted heparin-binding protein that shares ~35% amino acid (aa) identity with three other R‑Spondin family members (1‑3). All are positive modulators of Wnt/ beta -catenin signaling, but vary in activity (2). R‑Spondins regulate Wnt/ beta -catenin by competing with the Wnt antagonist DKK-1 for binding to the Wnt co-receptors LRP-6 and Kremen, reducing their DKK-1-mediated internalization (1, 4). Like other R‑spondins, human R‑Spondin 4 (228 aa) contains a signal sequence (aa 1‑19), two adjacent cysteine‑rich furin-like domains (aa 85-128) with one potential tyrosine phosphorylation site (aa 114), followed by a thrombospondin (TSP‑1) motif (aa 137‑197) and a region rich in basic residues (aa 199‑228). Mature human R‑Spondin 4 shares 81%, 81%, 84%, 84% and 86% aa identity with mouse, rat, equine, canine and bovine R‑Spondin 4, respectively. Of two potential isoforms, one lacks the TSP-1 domain, while another terminates at aa 224 (5). Each R‑Spondin has a distinct expression pattern (6). In the mouse, R‑Spondin 4 mRNA is found during development of limb bud mesenchyme, nail beds, heart and teeth (6‑8). In humans, mutations of R‑Spondin 4 have been found to cause anonychia, a condition in which fingernails and toenails are absent (8‑10).
  1. Nam, J.-S. et al. (2006) J. Biol. Chem. 281:13247.
  2. Kim, K.-A. et al. (2008) Mol. Biol. Cell 19:2588.
  3. Hendrickx, M. and L. Leyns (2008) Develop. Growth Differ. 50:229.
  4. Binnerts, M.E. et al. (2007) Proc. Natl. Acad. Sci. USA 104:14700.
  5. Entrez Accession # NP_001035096, EAX10652.
  6. Nam, J.-S. et al. (2007) Gene Expr. Patterns 7:306.
  7. Pemberton, T.J. et al. (2007) Dev. Dyn. 236:2245.
  8. Ishii, Y. et al. (2008) J. Invest. Dermatol. 128:867.
  9. Blaydon, D.C. et al. (2006) Nat. Genet. 38:1245.
  10. Bergmann, C. et al. (2006) Am. J. Hum. Genet. 79:1105.

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Publications for R-Spondin 4 (4575-RS/CF)(5)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Bioassay.

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Gene Symbol RSPO4