Recombinant Human Plexin C1 Protein, CF

• Reactivity: Hu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Human Plexin C1 Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human Plexin C1 is coated at 5 μg/mL, Recombinant Human Semaphorin 7A Fc Chimera (Catalog # 2068-S7) binds with an apparent K d <5 nM.
• Source: Mouse myeloma cell line, NS0-derived human Plexin C1 protein Ala35-Thr944, with a C-terminal 6-His tag
• Accession #: O60486
• N-terminal Sequence: Ala35
• Protein/Peptide Type: Recombinant Proteins
• Gene: PLXNC1
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 100.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 150-170 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 3 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Plexin C1 Protein, CF

• CD232 antigen
• CD232
• plexin (semaphorin receptor)
• Plexin C1
• PLXNC1
• PLXN-C1
• receptor for viral semaphorin protein
• receptor for virally-encoded semaphorin
• VESPR
• VESPRplexin-C1
• Virus-encoded semaphorin protein receptor

Background

Plexin C1, also known as Virus-encoded semaphorin protein receptor (VESPR) and CD232, is a 210 kDa type I transmembrane glycoprotein in the C subfamily of the Plexin family (1, 2). Human Plexin C1 contains a 34 amino acid (aa) signal sequence, a 910 aa extracellular domain (ECD) with one Sema-domain and two cysteine-rich Met-related sequences (MRS), a 21 aa transmembrane domain and a 603 aa cytoplasmic domain that includes features common to other plexins, including a phosphothreonine site. The human Plexin C1 ECD shares 85%, 82% and 71% aa identity with murine, bovine and opossum Plexin C1, respectively. Plexin C1 is widely expressed in neuronal and non-neuronal fetal and adult tissues (3). In neuronal development, its role is unclear. Semaphorin-7a (Sema 7A, CD108) binds Plexin C1 in vitro, and the two show a similar expression pattern during rat neuronal development. However, in rat, beta 1 integrins rather than Plexin C1 appear to mediate Sema 7A effects on axon outgrowth (4, 5). Plexin C1 does appear to play a role in the partitioning of paraventricular and supraoptic neurons in the hypothalamus, as indicated by specific defects seen in mice deleted for Plexin C1 (6). In the immune system, effects of Sema 7A on T cell-mediated inflammatory responses also appear to be mediated by beta 1 integrins rather than plexins (7). However, Plexin C1 may function to oppose the effect of beta 1 integrins, as it does on Sema 7A-mediated spreading and dendrite formation in melanocytes (8). Plexin C1 is the receptor for the poxvirus (A39R protein) and herpes virus (AHVsema) semaphorin homologs, and mediates activation of monocytes and inhibition of dendritic cell and neutrophil phagocytosis by A39R (2, 9, 10).

1. Negishi, M. et al. (2005) Cell. Mol. Life Sci. 62:1363. 
2. Comeau, M.R. et al. (1998) Immunity 8:473. 
3. Perala, N.M. et al. (2005) Gene Expr. Patterns 5:355. 
4. Pasterkamp, R.J. et al. (2007) BMC Dev. Biol. 7:98. 
5. Pasterkamp, R.J. et al. (2003) Nature 424:398. 
6. Xu, C. and C-M. Fan (2007) Mol. Endocrinol. 21:1234.
7. Suzuki, K. et al. (2007) Nature 446:680.
8. Scott, G.A. et al. (2007) J. Invest. Dermatol. 128:151.
9. Walzer, T. et al. (2005) J. Immunol. 174:51.
10. Walzer, T. et al. (2005) Eur. J. Immunol. 35:391.

Publications for Plexin C1 (3887-PC)(3)

Publications using 3887-PC

• S Esnault, EA Kelly Essential Mechanisms of Differential Activation of Eosinophils by IL-3 Compared to GM-CSF and IL-5 Crit. Rev. Immunol., 2016-01-01;36(5):429-444. 2016-01-01 [PMID: 28605348] (Bioassay, Human)
• S Esnault, EE Torr, K Bernau, MW Johansson, EA Kelly, N Sandbo, NN Jarjour Endogenous Semaphorin-7A Impedes Human Lung Fibroblast Differentiation PLoS ONE, 2017-01-17;12(1):e0170207. 2017-01-17 [PMID: 28095470] (Bioassay, Human)
• Peters E, Liezmann C, Spatz K, Ungethum U, Kuban R, Daniltchenko M, Kruse J, Imfeld D, Klapp B, Campiche R Profiling mRNA of the graying human hair follicle constitutes a promising state-of-the-art tool to assess its aging: an exemplary report. J Invest Dermatol, 2012-12-13;133(5):1150-60. 2012-12-13 [PMID: 23235529] (Bioassay, Human)

Bioinformatics

• Gene Symbol: PLXNC1
• Uniprot:
  • O60486()