Recombinant Human NRG1-beta 1/HRG1-beta 1 EGF Domain, CF

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1 μg/lane of Recombinant Human NRG1-beta 1/HRG1-beta 1 EGF Domain was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 8 kDa.
Recombinant Human NRG1-beta 1/HRG1-beta 1 EGF Domain (Catalog # 396-HB/CF) stimulates cell proliferation of the MCF-7 human breast cancer cell line. The ED50 for this effect is 0.06-0.3 ng/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications BA
Format
Carrier-Free

Order Details

Recombinant Human NRG1-beta 1/HRG1-beta 1 EGF Domain, CF Summary

Details of Functionality
Measured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083. The ED50 for this effect is 0.06-0.3 ng/mL.
Source
E. coli-derived
Thr176-Lys246
Accession #
N-terminal Sequence
Thr176
Protein/Peptide Type
Recombinant Proteins
Gene
NRG1
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
8 kDa, reducing conditions
Publications
Read Publications using
396-HB/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human NRG1-beta 1/HRG1-beta 1 EGF Domain, CF

  • Neuregulin1 beta 1
  • Neuregulin-1 beta 1

Background

The neuregulin family of structurally related glycoproteins comprises products from four distinct but related genes, Nrg-1, Nrg-2, Nrg-3, and Nrg-4. Through alternative splicing or the use of alternative promoters, Nrg-1 has been shown to encode more than 14 soluble or transmembrane proteins. The extracellular domain of the transmembrane NRG1 isoforms can be proteolytically cleaved to release soluble growth factors. All NRG1 isoforms contain an EGF-like domain ( alpha - or beta -splice variant that differ in their C-terminal region) that is required for their direct binding to the ErbB3 or ErbB4 receptor tyrosine kinases. The ErbB3 or ErbB4 subsequently recruits and heterodimerizes with ErbB2, resulting in tyrosine phosphorylation and NRG1 signaling. NRG1 isoforms can be classified into three major subtypes. Type I (Neu Differentiation Factor, NDF; Heregulin, HRG; Acetylcholine Receptor Inducing Activity, ARIA) and type II (Glial Growth Factor, GGF) NRG1s have an immunoglobulin (Ig)-like domain N-terminal to the EGF-like domain. Type I NRG1s differ from type II NRG1s by having a glycosylation-rich domain between the Ig-like and the EGF-like domains. Type III NRG1s (Sensory and Motor neuron-Derived Factor) lacks the Ig-like domain but has a cysteine rich domain (CRD) instead. NRG1 isoforms show distinct spatial and temporal expression patterns. These proteins play important roles during development of both the nervous system and the heart. They have been shown to regulate the selective expression of neurotransmitter receptors in neurons and at the neuromuscular junction, and promote the differentiation and development of Schwann cells from neural crest stem cells. NRG1s have also been shown to be involved in the establishment of the oligodendroglial lineage.

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Publications for (30)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Hamster.

We have publications tested in 3 applications: Bioassay, In Vivo, cell culture.


Filter By Application
Bioassay
(19)
In Vivo
(9)
cell culture
(1)
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Filter By Species
Human
(12)
Mouse
(9)
Rat
(8)
Hamster
(1)
All Species
Showing Publications 1 - 10 of 30. Show All 30 Publications.
Publications using 396-HB/CF Applications Species
S Razavi, N Ghasemi, M Mardani, H Salehi Remyelination improvement after neurotrophic factors secreting cells transplantation in rat spinal cord injury Iran J Basic Med Sci, 2017;20(4):392-398. 2017 [PMID: 28804608] (Bioassay, Human) Bioassay Human
H Uchida, M Machida, T Miura, T Kawasaki, T Okazaki, K Sasaki, S Sakamoto, N Ohuchi, M Kasahara, A Umezawa, H Akutsu A xenogeneic-free system generating functional human gut organoids from pluripotent stem cells JCI Insight, 2017;2(1):e86492. 2017 [PMID: 28097227] (Bioassay, Human) Bioassay Human
DW Chung, ZP Wills, KN Fish, DA Lewis Developmental pruning of excitatory synaptic inputs to parvalbumin interneurons in monkey prefrontal cortex Proc. Natl. Acad. Sci. U.S.A, 2017;114(4):E629-E637. 2017 [PMID: 28074037] (Bioassay, Human) Bioassay Human
HRG/HER2/HER3 signaling promotes AhR-mediated Memo-1 expression and migration in colorectal cancer Oncogene, 2016;0(0):. 2016 [PMID: 27941874] (Bioassay, Human) Bioassay Human
Myelinating glia differentiation is regulated by extracellular matrix elasticity Sci Rep, 2016;6(0):33751. 2016 [PMID: 27646171] (Bioassay, Rat) Bioassay Rat
Regulation of inflammatory responses by neuregulin-1 in brain ischemia and microglial cells in vitro involves the NF-kappa B pathway J Neuroinflammation, 2016;13(1):237. 2016 [PMID: 27596278] (Bioassay, Mouse) Bioassay Mouse
Heregulin-1� and HER3 in hepatocellular carcinoma: status and regulation by insulin J Exp Clin Cancer Res, 2016;35(1):126. 2016 [PMID: 27514687] (Bioassay, Human) Bioassay Human
The reemergence of long-term potentiation in aged Alzheimer&#039;s disease mouse model Sci Rep, 2016;6(0):29152. 2016 [PMID: 27377368] (Bioassay, Mouse) Bioassay Mouse
Lai Man Natalie Wu Zeb2 recruits HDAC-NuRD to inhibit Notch and controls Schwann cell differentiation and remyelination Nat Neurosci, 2016;0(0):. 2016 [PMID: 27294509] (Bioassay, Rat) Bioassay Rat
Activation of endothelial ?-catenin signaling induces heart failure Sci Rep, 2016;6(0):25009. 2016 [PMID: 27146149] (In Vivo, Mouse) In Vivo Mouse
Show All 30 Publications.

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Bioinformatics

Gene Symbol NRG1
Entrez
Uniprot