Recombinant Human NRG1-beta 1/HRG1-beta 1 EGF Domain, CF

Images

 
1 μg/lane of Recombinant Human NRG1-beta 1/HRG1-beta 1 EGF Domain was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 8 kDa.
Recombinant Human NRG1-beta 1/HRG1-beta 1 EGF Domain (Catalog # 396-HB/CF) stimulates cell proliferation of the MCF-7 human breast cancer cell line. The ED50 for this effect is 0.06-0.3 ng/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Recombinant Human NRG1-beta 1/HRG1-beta 1 EGF Domain, CF Summary

Details of Functionality
Measured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083. The ED50 for this effect is 0.06-0.3 ng/mL.
Source
E. coli-derived human Neuregulin-1 beta 1/NRG1 beta 1 protein
Thr176-Lys246
Accession #
N-terminal Sequence
Thr176
Protein/Peptide Type
Recombinant Proteins
Gene
NRG1
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
8 kDa, reducing conditions
Publications
Read Publications using
396-HB/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human NRG1-beta 1/HRG1-beta 1 EGF Domain, CF

  • ARIA
  • GGF
  • GGF2
  • HGL
  • HRG
  • HRG1
  • HRGA
  • MST131
  • MSTP131
  • NDF
  • neuregulin 1
  • Neuregulin1 beta 1
  • Neuregulin-1 beta 1
  • NRG1-IT2
  • SMDF

Background

The neuregulin family of structurally related glycoproteins comprises products from four distinct but related genes, Nrg-1, Nrg-2, Nrg-3, and Nrg-4. Through alternative splicing or the use of alternative promoters, Nrg-1 has been shown to encode more than 14 soluble or transmembrane proteins. The extracellular domain of the transmembrane NRG1 isoforms can be proteolytically cleaved to release soluble growth factors. All NRG1 isoforms contain an EGF-like domain ( alpha - or beta -splice variant that differ in their C-terminal region) that is required for their direct binding to the ErbB3 or ErbB4 receptor tyrosine kinases. The ErbB3 or ErbB4 subsequently recruits and heterodimerizes with ErbB2, resulting in tyrosine phosphorylation and NRG1 signaling. NRG1 isoforms can be classified into three major subtypes. Type I (Neu Differentiation Factor, NDF; Heregulin, HRG; Acetylcholine Receptor Inducing Activity, ARIA) and type II (Glial Growth Factor, GGF) NRG1s have an immunoglobulin (Ig)-like domain N-terminal to the EGF-like domain. Type I NRG1s differ from type II NRG1s by having a glycosylation-rich domain between the Ig-like and the EGF-like domains. Type III NRG1s (Sensory and Motor neuron-Derived Factor) lacks the Ig-like domain but has a cysteine rich domain (CRD) instead. NRG1 isoforms show distinct spatial and temporal expression patterns. These proteins play important roles during development of both the nervous system and the heart. They have been shown to regulate the selective expression of neurotransmitter receptors in neurons and at the neuromuscular junction, and promote the differentiation and development of Schwann cells from neural crest stem cells. NRG1s have also been shown to be involved in the establishment of the oligodendroglial lineage.

  1. Buonanno, A., and Fischbach, G.D. (2001) Curr. Opin. Neurobiol. 11:287.
  2. Adlkofer, K. and Lai, C. (2000) Glia 29:104.
  3. Garratt, A.N. et al. (2000) BioEssays 22:987.

Customers Who Viewed This Item Also Viewed...

NBP1-33120
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, IP, WB
AF231
Species: Hu
Applications: CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, ICC, IHC, IP, Simple Western, WB
MAB6967
Species: Hu
Applications: WB
1129-ER
Species: Hu
Applications: BA
MAB3481
Species: Hu
Applications: CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, Neut
236-EG
Species: Hu
Applications: BA
NBP2-52983
Species: Hu, Mu
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NBP2-00527
Species: Ca, Hu, Pm, Mu, Pm
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NBP1-33177
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, KO, WB
NBP3-15868
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
NBP1-85299
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
AF887
Species: Hu, Mu, Rt
Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB
M1300CB
Species: Mu
Applications: ELISA
NBP2-67360
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IP, KO, WB
NB110-40773
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P
NB100-1780
Species: Hu, Mu
Applications: Flow, PEP-ELISA, WB
NBP2-80405
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
396-HB/CF
Species: Hu
Applications: Bioactivity

Publications for Neuregulin-1 beta 1/NRG1 beta 1 (396-HB/CF)(64)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Canine, Chinese Hamster, Hamster.

We have publications tested in 4 applications: Bioassay, Cell Culture, In Vivo, Stimulation.


Filter By Application
Bioassay
(44)
Cell Culture
(5)
In Vivo
(13)
Stimulation
(1)
All Applications
Filter By Species
Human
(24)
Mouse
(19)
Rat
(18)
Canine
(1)
Chinese Hamster
(1)
Hamster
(1)
All Species
Showing Publications 1 - 10 of 64. Show All 64 Publications.
Publications using 396-HB/CF Applications Species
J Patritti C, J Wu, RA Coover, TA Rizvi, KE Chaney, R Ravindran, JA Cancelas, RJ Spinner, N Ratner P2RY14 cAMP signaling regulates Schwann cell precursor self-renewal, proliferation, and nerve tumor initiation in a mouse model of neurofibromatosis Elife, 2022;11(0):. 2022 [PMID: 35311647] (Cell Culture, Mouse) Cell Culture Mouse
K Kim, D Lee ERBB3-dependent AKT and ERK pathways are essential for atrioventricular cushion development in mouse embryos PLoS ONE, 2021;16(10):e0259426. 2021 [PMID: 34714866] (Bioassay, Chinese Hamster) Bioassay Chinese Hamster
C Ma, W Zhang, W Wang, J Shen, K Cai, M Liu, M Cao SKP-SCs transplantation alleviates 6-OHDA-induced dopaminergic neuronal injury by modulating autophagy Cell Death & Disease, 2021;12(7):674. 2021 [PMID: 34226513] (Bioassay, Rat) Bioassay Rat
G Della-Flor, ER Wilson, LN Marziali, E Hurley, N Silvestri, B He, BW O'Malley, B Beirowski, Y Poitelon, L Wrabetz, ML Feltri Prohibitin 1 is essential to preserve mitochondria and myelin integrity in Schwann cells Nature Communications, 2021;12(1):3285. 2021 [PMID: 34078899] (Bioassay, Mouse) Bioassay Mouse
X Zheng, KJ Salinas, DXF Velez, T Nakayama, X Lin, D Banerjee, X Xu, SP Gandhi Host interneurons mediate plasticity reactivated by embryonic inhibitory cell transplantation in mouse visual cortex Nature Communications, 2021;12(1):862. 2021 [PMID: 33558487] (In Vivo, Mouse) In Vivo Mouse
HJ Seo, JE Park, SM Choi, T Kim, SH Cho, KH Lee, WK Song, J Song, HS Jeong, DH Kim, BC Kim Inhibitory Neural Network&#039;s Impairments at Hippocampal CA1 LTP in an Aged Transgenic Mouse Model of Alzheimer&#039;s Disease International Journal of Molecular Sciences, 2021;22(2):. 2021 [PMID: 33445678] (Bioassay, Mouse) Bioassay Mouse
R Fujii-Tezu, M Ishige-Wad, N Nagoshi, H Okano, H Mugishima, S Takahashi, I Morioka, T Matsumoto Umbilical artery tissue contains p75 neurotrophin receptor-positive pericyte-like cells that possess neurosphere formation capacity and neurogenic differentiation potential Regenerative Therapy, 2021;16(0):1-11. 2021 [PMID: 33426237] (Bioassay, Human) Bioassay Human
JE Cohen, AB Goldstone, H Wang, BP Purcell, Y Shudo, JW MacArthur, AN Steele, MJ Paulsen, BB Edwards, CN Aribeana, NC Cheung, JA Burdick, YJ Woo A Bioengineered Neuregulin-Hydrogel Therapy Reduces Scar Size and Enhances Post-Infarct Ventricular Contractility in an Ovine Large Animal Model J Cardiovasc Dev Dis, 2020;7(4):. 2020 [PMID: 33212844] (Cell Culture, Human) Cell Culture Human
PG Mazzara, S Muggeo, M Luoni, L Massimino, M Zaghi, PT Valverde, S Brusco, MJ Marzi, C Palma, G Colasante, A Iannielli, M Paulis, C Cordiglier, SG Giannelli, P Podini, C Gellera, F Taroni, F Nicassio, M Rasponi, V Broccoli Frataxin gene editing rescues Friedreich&#039;s ataxia pathology in dorsal root ganglia organoid-derived sensory neurons Nat Commun, 2020;11(1):4178. 2020 [PMID: 32826895] (Bioassay, Human) Bioassay Human
PG Mazzara, S Muggeo, M Luoni, L Massimino, M Zaghi, PT Valverde, S Brusco, MJ Marzi, C Palma, G Colasante, A Iannielli, M Paulis, C Cordiglier, SG Giannelli, P Podini, C Gellera, F Taroni, F Nicassio, M Rasponi, V Broccoli Frataxin gene editing rescues Friedreich&#039;s ataxia pathology in dorsal root ganglia organoid-derived sensory neurons Nat Commun, 2020;11(1):4178. 2020 [PMID: 32826895] (Bioassay, Human) Bioassay Human
Show All 64 Publications.

Reviews for Neuregulin-1 beta 1/NRG1 beta 1 (396-HB/CF) (0)

There are no reviews for Neuregulin-1 beta 1/NRG1 beta 1 (396-HB/CF). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for Neuregulin-1 beta 1/NRG1 beta 1 (396-HB/CF) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Neuregulin-1 beta 1/NRG1 beta 1 Products

Bioinformatics Tool for Neuregulin-1 beta 1/NRG1 beta 1 (396-HB/CF)

Discover related pathways, diseases and genes to Neuregulin-1 beta 1/NRG1 beta 1 (396-HB/CF). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Neuregulin-1 beta 1/NRG1 beta 1 (396-HB/CF)

Discover more about diseases related to Neuregulin-1 beta 1/NRG1 beta 1 (396-HB/CF).
 

Pathways for Neuregulin-1 beta 1/NRG1 beta 1 (396-HB/CF)

View related products by pathway.

PTMs for Neuregulin-1 beta 1/NRG1 beta 1 (396-HB/CF)

Learn more about PTMs related to Neuregulin-1 beta 1/NRG1 beta 1 (396-HB/CF).

Blogs on Neuregulin-1 beta 1/NRG1 beta 1

There are no specific blogs for Neuregulin-1 beta 1/NRG1 beta 1, but you can read our latest blog posts.
Omicron Variant Antibodies

Customers Who Bought This Also Bought

IL-13 [HRP]
M1300CB

Contact Information

Download our Western Blot eHandbook

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human NRG1-beta 1/HRG1-beta 1 EGF Domain, CF and receive a gift card or discount.

Bioinformatics

Gene Symbol NRG1
Uniprot