Recombinant Human NRG1-alpha/HRG1-alpha EGF Domain Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human NRG1-alpha/HRG1-alpha EGF Domain Protein Summary

Details of Functionality
Measured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083. The ED50 for this effect is 10‑50 ng/mL.
Source
E. coli-derived human Neuregulin-1 alpha/NRG1 alpha protein
Ser177-Lys241
Accession #
N-terminal Sequence
Ser177
Protein/Peptide Type
Recombinant Proteins
Gene
NRG1
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
8 kDa, reducing conditions
Publications
Read Publications using
296-HR in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human NRG1-alpha/HRG1-alpha EGF Domain Protein

  • ARIA
  • GGF2
  • HGL
  • HRG
  • HRG1
  • MST131
  • MSTP131
  • neuregulin 1
  • Neuregulin1 alpha
  • Neuregulin-1 alpha
  • pro-neuregulin-1, membrane-bound isoform
  • pro-NRG1
  • sensory and motor neuron derived factor
  • SMDF

Background

The neuregulin family of structurally related glycoproteins comprises products from four distinct but related genes, Nrg-1, Nrg-2, Nrg-3, and Nrg-4. Through alternative splicing or the use of alternative promoters, Nrg-1 encodes more than 14 soluble or transmembrane proteins. The extracellular domain of the transmembrane NRG1 isoforms can be proteolytically cleaved to release soluble growth factors. The alpha - or  beta -splice variants differ in their C-terminal region. All NRG1 isoforms contain an EGF-like domain that is required for their direct binding to the ErbB3 or ErbB4 receptor tyrosine kinases. The ErbB3 or ErbB4 subsequently recruits and heterodimerizes with ErbB2, resulting in tyrosine phosphorylation and NRG1 signaling. NRG1 isoforms can be classified into three major subtypes. Type I (Neu Differentiation Factor, NDF; Heregulin, HRG; Acetylcholine Receptor Inducing Activity, ARIA) and type II (Glial Growth Factor, GGF). NRG1s have an immunoglobulin (Ig)-like domain N-terminal to the EGF-like domain. Type I NRG1s differ from type II NRG1s by having a glycosylation-rich domain between the Ig-like and the EGF-like domains. Type III NRG1 (Sensory and Motor neuron-Derived Factor) lacks the Ig-like domain but has a cysteine rich domain (CRD) instead. NRG1 isoforms show distinct spatial and temporal expression patterns. These proteins play important roles during development of both the nervous system and the heart. They have been shown to regulate the selective expression of neurotransmitter receptors in neurons and at the neuromuscular junction, and to promote the differentiation and development of Schwann cells from neural crest stem cells. NRG1s have also been shown to be involved in the establishment of the oligodendroglial lineage.

  1. Lemke, G. (2006) Science STKE 325:pe11.
  2. Esper, R.M. et al. (2006) Brain Res. Brain Res. Rev. 51:161.
  3. Tosato, S. et al. (2005) Schizophr. Bull. 31:613.

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296-HR
Species: Hu
Applications: Bioactivity

Publications for Neuregulin-1 alpha/NRG1 alpha (296-HR)(11)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 2 applications: Bioassay, In Vivo.


Filter By Application
Bioassay
(9)
In Vivo
(2)
All Applications
Filter By Species
Human
(4)
Mouse
(4)
Rat
(3)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using 296-HR Applications Species
M Shu, H Yan, C Xu, Y Wu, Z Chi, W Nian, Z He, J Xiao, H Wei, Q Zhou, JX Zhou A novel anti-HER2 antibody GB235 reverses Trastuzumab resistance in HER2-expressing tumor cells in vitro and in vivo Sci Rep, 2020;10(1):2986. 2020 [PMID: 32076029] (Bioassay, Human) Bioassay Human
M Morano, G Ronchi, V Nicolò, BE Fornasari, A Crosio, I Perroteau, S Geuna, G Gambarotta, S Raimondo Modulation of the Neuregulin 1/ErbB system after skeletal muscle denervation and reinnervation Sci Rep, 2018;8(1):5047. 2018 [PMID: 29568012] (Bioassay, Mouse) Bioassay Mouse
Benedetta Elena Fornasari Neuregulin1 alpha activates migration of neuronal progenitors expressing ErbB4 Mol. Cell. Neurosci, 2016;0(0):. 2016 [PMID: 27989735] (Bioassay, Rat) Bioassay Rat
Solomon W, Wilson N, Anderson L, Pitts S, Patrickson J, Liu M, Ford B, Stiles J Neuregulin-1 attenuates mortality associated with experimental cerebral malaria. J Neuroinflammation, 2014;11(0):9. 2014 [PMID: 24433482] (In Vivo, Mouse) In Vivo Mouse
MacLeod S, Elgadi M, Bossi G, Sankar U, Pisio A, Agopsowicz K, Sharon D, Graham F, Hitt M HER3 targeting of adenovirus by fiber modification increases infection of breast cancer cells in vitro, but not following intratumoral injection in mice. Cancer Gene Ther, 2012;19(12):888-98. 2012 [PMID: 23099884] (Bioassay, Human) Bioassay Human
Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 2012;487(7408):505-9. 2012 [PMID: 22763448] (Bioassay, Human) Bioassay Human
Zhu J, Jia X, Xiao G, Kang Y, Partridge NC, Qin L EGF-like ligands stimulate osteoclastogenesis by regulating expression of osteoclast regulatory factors by osteoblasts: implications for osteolytic bone metastases. J. Biol. Chem., 2007;282(37):26656-64. 2007 [PMID: 17636266] (Bioassay, Mouse) Bioassay Mouse
Wehrman TS, Raab WJ, Casipit CL, Doyonnas R, Pomerantz JH, Blau HM A system for quantifying dynamic protein interactions defines a role for Herceptin in modulating ErbB2 interactions. Proc. Natl. Acad. Sci. U.S.A., 2006;103(50):19063-8. 2006 [PMID: 17148612] (Bioassay, Mouse) Bioassay Mouse
Hamra FK, Chapman KM, Nguyen D, Garbers DL Identification of neuregulin as a factor required for formation of aligned spermatogonia. J. Biol. Chem., 2007;282(1):721-30. 2007 [PMID: 17098736] (Bioassay, Rat) Bioassay Rat
Chakravarti P, Henry MK, Quelle FW Prolactin and heregulin override DNA damage-induced growth arrest and promote phosphatidylinositol-3 kinase-dependent proliferation in breast cancer cells. Int. J. Oncol., 2005;26(2):509-14. 2005 [PMID: 15645137] (Bioassay, Human) Bioassay Human
Show All 11 Publications.

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Bioinformatics

Gene Symbol NRG1
Uniprot