Recombinant Human Nidogen-2 Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human Nidogen-2 Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to support the adhesion of SVEC4‑10 mouse vascular endothelial cells. When 4 x 104 cells/well are added to Recombinant Human Nidogen-2 coated plates (30 µg/mL with 100 µL/well), approximately 30-50% will adhere after one hour at 37° C.
Optimal dilutions should be determined by each laboratory for each application.
Source
Chinese Hamster Ovary cell line, CHO-derived human Nidogen-2 protein
Leu31-Lys1375 (Gly832Ala), with an N-terminal 9-His tag
Accession #
N-terminal Sequence
His
Protein/Peptide Type
Recombinant Proteins
Gene
NID2
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
149.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
140-190 kDa, reducing conditions
Publications
Read Publication using
3385-ND in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Nidogen-2 Protein, CF

  • NID2
  • NID-2
  • nidogen 2 (osteonidogen)
  • Nidogen2
  • Nidogen-2
  • Osteonidogen

Background

Nidogen-2 (also named entactin-2) is a 200 kDa, secreted, monomeric basement membrane glycoprotein (1). Nidogens-1 and 2 are expressed in nearly all basement membranes (1-3) where they interact with laminins, collagen type IV and proteoglycan family members to form structural scaffolds (4, 5). In mouse, Nidogens 1 and 2 appear to substitute for each other. Deletion of one nidogen gives a mild phenotype, but deletion of both nidogens is lethal (6, 7). Affinity of laminin binding is much lower for human Nidogen-2 than that of mouse Nidogen-2, indicating that human Nidogen-2 may not be a strict substitute for Nidogen-1 (1). Both nidogens bind perlecan and collagens I and IV, but only Nidogen-1 binds fibulins (1, 3). The two nidogens show approximately 50% amino acid (aa) identity in human and are structurally similar (1, 4, 6). Cleavage of a 28 aa signal sequence from human Nidogen-2 produces a 1219 aa mature protein containing three globular domains
(G1-3) separated by a link region and an extended rod-shaped segment. The G1 domain is reported to bind type IV collagen, the G2 Nidogen ( beta -barrel) domain interacts with perlecan, and the C-terminal G3 beta -propeller structure is associated with laminin binding. The mucin-like link region is longer in Nidogen-2 than nidogen-1, and contains both N- and O-glycosylation (2, 8). There is one EGF-like motif and a short peptide that ligates alpha 3 beta 1 integrins. The rod-shaped segment contains four additional EGF-like motifs, two of which bind calcium, and two thyroglobulin type 1 domains that serve as a binding site for alpha v beta 3 integrins. Mature human Nidogen-2 is 80% aa identical to both mouse and rat Nidogen-2, and 73% aa identical to both canine and bovine Nidogen-2.

  1. Kohfeldt, K. et al. (1998) J. Mol. Biol. 282:99.
  2. Miosge, N. et al. (2001) Histochem. J. 33:523.
  3. Salmivirta, K. et al. (2002) Exp. Cell Res. 279:188.
  4. Hohenester, E. and J. Engel (2002) Matrix Biol. 21:115.
  5. Charonis, A. et al. (2005) Curr. Med. Chem. 12:1495.
  6. Schymeinsky, J. et al. (2002) Mol. Cell. Biol. 22:6820.
  7. Bader, B.L. et al. (2005) Mol. Cell. Biol. 25:6846.

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Publications for Nidogen-2 (3385-ND)(1)

We have publications tested in 1 confirmed species: Bacteria.

We have publications tested in 1 application: Binding Assay.


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Bioinformatics

Gene Symbol NID2
Uniprot