Recombinant Human Neuregulin-3/NRG3 His-tag Protein, CF

Measured by its binding ability in a functional ELISA. Recombinant Human Neuregulin‑3/NRG3 His-tag Protein (Catalog # 11520-NR) binds to Recombinant Human ErbB4/Her4 Fc Chimera Protein (1131-ER) with anED50 of 5.00-50.0 ng/mL.

2 μg/lane of Recombinant Human Neuregulin‑3/NRG3 His-tag Protein (Catalog # 11520-NR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 35-74 kDa, under reducing conditions.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Neuregulin-3/NRG3 His-tag Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. Recombinant Human Neuregulin‑3/NRG3 His-tag (Catalog # 11520-NR) binds to Recombinant Human ErbB4/Her4 Fc Chimera (Catalog # 1131-ER) with an ED₅₀ of 5.00-50.0 ng/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived human Neuregulin-3/NRG3 protein
  Ser93-Arg359, with a C-terminal 6-His tag
• Accession #: P56975.1
• N-terminal Sequence: Ser93 and Leu158
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 30 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 35-74 kDa, under reducing conditions.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Neuregulin-3/NRG3 His-tag Protein, CF

• HRG3
• neuregulin 3 variant 1
• neuregulin 3 variant 10
• neuregulin 3 variant 11
• neuregulin 3 variant 12
• neuregulin 3 variant 13
• neuregulin 3 variant 14
• neuregulin 3 variant 3
• neuregulin 3 variant 4
• neuregulin 3 variant 5
• neuregulin 3 variant 6
• neuregulin 3 variant 7
• neuregulin 3 variant 8
• neuregulin 3 variant 9
• neuregulin 3
• Neuregulin3
• Neuregulin-3
• neuregulin-3-like polypeptide
• NRG3
• pro-neuregulin-3, membrane-bound isoform
• pro-NRG3

Background

Neuregulin-3 is a member of the neuregulin family of EGF proteins. Human NRG3, sharing 93% homology with mouse and rat NRG3, is a 78 kDa, 720 aa protein containing a 339 aa cytoplasmic domain, a 21 aa transmembrane domain, and a 360 extracellular domain with an epidermal growth factor (EGF) motif which acts as a a ligand for the ErbB4 receptor tyrosine kinase that plays pleotropic roles in neurodevelopment (1-2). Unlike many NRG1 family members but similar to sensory and motor neuron-derived factor (SMDF), NRG3 does not contain Ig-like or kringle-like domains but does contain a stretch of nonpolar aa (W66–V91). This region is thought to help translocation across the endoplasmic reticulum. NRG3 also lacks sites for N-linked glycosylation but contains many O-linked sites in a mucin-like Ser/Thr rich region. NRG3-ErbB4 was implicated in the signaling in neurodevelopment and adult brain function by promoting oligodendrocyte survival via the ErbB4/PI3K/AKT1 pathway (3). NRG3 and NRG1 can serve as potential independent biomarkers in brain lower grade glioma and glioblastoma multiforme (4). Several studies have proved that NRG1 and NRG3 play important roles in brain development, including neural plasticity (5), differentiation (6), and Schwann cell migration (7). It was also shown that NRG3 signaling acts to promote the initiation of mammary placode development and promoted the differentiation of squamous epithelia into the mammary epithelia (8).

1. Zhang, D., et al. Proc. Natl. Acad. Sci. USA. 1997. 94:9562-9567.
2. Kao, W-T. et al. PNAS. 2010. 107(35):15619-15624.
3. Carteron, C. et al. J Cell Sci. 2006. 119:898-909.
4. Zhao, W. et al. Front. Immunol. 2021. 12:682415.
5. Long, LE., et al. PLoS One. 2015. 10:e0124114. doi: 10.1371/journal.pone.0124114.
6. Zhou, Y. et al. Transl Psychiatry. 2020. 10:62. doi: 10.1038/s41398-020-0746-5.
7. Perlin, JR. et al. Development. 2011. 138:4639–48. doi: 10.1242/dev.068072.
8. Howard, B. et al. Genes Dev. 2005. 19:2078-90.

Bioinformatics

• Uniprot:
  • P56975.1()