Recombinant Human Neudesin Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Neudesin Protein, CF Summary

• Details of Functionality: Measured in a cell proliferation assay using Neuro‑2A mouse neuroblastoma cells. The ED₅₀ for this effect is 1.5-6.0 μg/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived human Neudesin protein
  Gly32-Phe172, with a C-terminal 6-His tag
• Accession #: Q9UMX5
• N-terminal Sequence: Starts at Gly32
• Structure / Form: Monomer
• Protein/Peptide Type: Recombinant Proteins
• Gene: NENF
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Theoretical MW: 16.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 19-21 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Neudesin Protein, CF

• Cell immortalization-related protein 2
• CIR2
• CIR2cell growth-inhibiting protein 47
• NENF
• Neudesin
• neuron derived neurotrophic factor
• Neuron-derived neurotrophic factor
• SCIRP10
• SCIRP10-related protein
• Secreted protein of unknown function
• Spinal cord injury related protein 10
• SPUF protein
• SPUF
• SPUFneudesin

Background

Neudesin (neuron‑derived neurotrophic secreted protein), gene name NENF (neudesin neurotrophic factor), also called CIR2 (cell immortalization‑related 2) or GIG47, is a secreted, 20‑21 kDa member of the MAPR (membrane‑associated progesterone receptor) subfamily of the cytochrome b5 family of molecules (1, 2). Human Neudesin is synthesized as a 172 amino acid (aa) precursor that contains a 31 aa signal sequence with a 141 aa mature region that possesses a cytochrome b5‑like heme‑binding domain over aa 44‑129, and a lysine acetylation site at aa 136 (1‑4). The attachment of hemin to its heme‑binding domain is necessary for its neurotrophic activity, and the binding of heme accounts for 5‑6 kDa of its circulating molecular weight (3). Mature human Neudesin shares 97%, 94% and 96% aa identity with mouse, rat, and bovine Neudesin, respectively. Neudesin appears to selectively promote cell survival or proliferation and inhibit differentiation in multiple settings. It is expressed by neuronal progenitors and neurons in the central nervous system, and by preadipocytes in white adipose tissue (5, 6). It promotes neuronal differentiation with limited proliferation and serves as a neuron survival factor, but inhibits both astrocyte and adipocyte differentiation (1, 5, 6). Neudesin over‑expression is found in tumors in the human breast, cervix, colon, lung and skin, and in human tumor cell lines that include some lymphomas and leukemias; transfection studies indicate that it may act as an oncogene (4, 7).

1. Kimura, I. et al. (2005) J. Neurosci. Res. 79:287.
2. Ma, L. et al. (1998) Oncogene 17:1321.
3. Kimura, I. et al. (2008) J. Biol. Chem. 283:4323.
4. Han, K.H. et al. (2012) BMC Cancer 12:274.
5. Kimura, I. et al. (2006) J. Neurosci. Res. 83:1415.
6. Kimura, I. et al. (2009) Biochem. Biophys. Res. Commun. 381:75.
7. Neubauer, H. et al. (2006) Electrophoresis 27:1840.

Bioinformatics

• Gene Symbol: NENF
• Uniprot:
  • Q9UMX5(Human)