Recombinant Human Netrin-G2 His-tag Protein, CF

When Recombinant Human Netrin‑G2 His-Tag (Catalog # 10675-NG) is coated at 1 µg/mL, Recombinant Human LRRC4 Fc Chimera (9375-LR) binds with an ED50 of 1.5-9 ng/mL.

2 μg/lane of Recombinant Human Netrin-G2 His-tag (Catalog # 10675-NG) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 70-78 kDa.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Netrin-G2 His-tag Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human Netrin-G2 His-Tag protein is coated at 1 µg/mL, Recombinant Human LRRC4 Fc Chimera (Catalog # 9375-LR) binds with an ED 50 of 1.5-9 ng/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived human Netrin-G2 protein Asp18-Pro506, with a C-terminal 6-His tag
• Accession #: Q96CW9.2
• N-terminal Sequence: Asp18
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 56 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 70-78 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 3 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 1 mg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Netrin-G2 His-tag Protein, CF

• bA479K20.1
• KIAA0625
• KIAA1857bA479K20.1 (novel protein)
• laminet 2
• laminet-2
• LHLL9381
• Lmnt2
• MGC21884
• netrin G1
• netrin G2
• Netrin-G2
• NTNG1
• NTNG2

Background

NTNG-2 (Netrin-G2) is a member of a GPI-linked Netrin subfamily that is distantly related to classical Netrins. Mature human Netrin-G2 is approximately 490 amino acid (aa) in length. It contains an N-terminal laminin-related region, several epidermal-growth-factor-like domains and a heparin-binding C-terminus that ends in a GPI-linkage (1). The laminin-related region shows a globular type VI domain followed by three variable-length EGF-like domains. Mature human Netrin-G2 shares 96% aa identity with mouse and rat Netrin-G2. NTNG-2 is predominantly expressed in specific neuronal subsets of the developing and mature central nervous system (2). The protein interacts with the extracellular region of their specific Netrin-G ligand receptors LRRC4 (3). The netrin family proteins provide axonal guidance cues during central nervous system development (4).

1. Seiradake E. et al. (2011) The EMBO J. 30:4479.
2. Pirone A. et al. (2012) PLOS 7(9):e44745.
3. Woo, J. et al. Mol. Cell Neurosci. (2009) 42:1.
4. Zhang, Q. et al. (2005) FEBS Lett. 579:3674.

Bioinformatics

• Uniprot:
  • Q96CW9.2()