Recombinant Human NETO1 His-tag Protein, CF

2 μg/lane of Recombinant Human NETO1 His-tag Protein (Catalog # 11103-NO) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 34-48 kDa.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human NETO1 His-tag Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human NETO1 His-tag Protein is immobilized at 4.00 µg/mL (100 µL/well), Recombinant Human APP695⁺¹ Fc Chimera binds with an ED₅₀ of 0.200-1.20 μg/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived human NETO1 protein
  Thr23-Val345, with a C-terminal 6-His tag
• Accession #: Q8TDF5.1
• N-terminal Sequence: Thr23
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 37 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 34-48 kDa, under reducing conditions.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human NETO1 His-tag Protein, CF

• BCTL1
• Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor classA domains protein 1
• BTCL1
• BTCL1BCTL1neuropilin and tolloid-like protein 1
• FLJ41325
• NETO1
• neuropilin (NRP) and tolloid (TLL)-like 1

Background

Neuropilin and tolloid-like protein 1 (NETO1), also known as Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 1 (BTCL1), is a type I transmembrane protein that is expressed in the brain and retina (1). The 511 amino acid (aa) residue mature human NETO1 contains a 322 aa extracellular domain (ECD) with two CUB domains and one class A LDL-receptor segment, a 21 aa transmembrane domain, and a 168 aa cytosol domain (1). Three human NETO1 splice isoforms exist, including a secreted soluble variant that shows an Asp-to-Glu transition at residue 157 followed by a premature truncation (1). The ECD of human NETO1 shares 97% and 98% aa identity with that of mouse and canine NETO1, respectively. NETO1 is expressed in the human brain and retina (1, 2). It increases the rate of GluK1 receptor desensitization and is a candidate NMDAR auxiliary subunit regulating channel activities and synaptic transmission (3, 4). NETO1 expression is associated with metastasis and poor survival in epithelial ovarian cancer (5).

1. Stöhr, H. et al. (2002) Gene 286:223.
2. Tomita, S. and P.E. Castillo (2012) J. Physiol. 590:2217.
3. Copits, B.A. et al. (2011) J. Neurosci. 31:7334.
4. Ng, D. et al. (2009) PloS Biol. 7:e41.
5. Xu, Y. et al. (2020) J. Cell. Mol. Med. 24:9114.

Bioinformatics

• Uniprot:
  • Q8TDF5.1()