Recombinant Human/Mouse Frizzled-2 Fc Chimera Protein, CF

• Reactivity: Hu, Mu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Human/Mouse Frizzled-2 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its ability to bind biotinylated Wnt-5a in a functional ELISA with an estimated K_d <5 nM.
• Source: Mouse myeloma cell line, NS0-derived Frizzled-2 protein
  

  Human/Mouse Frizzled-2 (Gln29-Leu168) Accession # Q9JIP6	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: Q9JIP6
• N-terminal Sequence: No results obtained: Gln29 predicted
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: FZD2
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 42.3 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 50-55 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human/Mouse Frizzled-2 Fc Chimera Protein, CF

• frizzled (Drosophila) homolog 2
• frizzled homolog 2 (Drosophila)
• Frizzled2
• Frizzled-2
• Fz2
• fz-2
• FZD2
• fzE2
• hFz2

Background

Wnt signaling is involved in variety of developmental processes including cell fate determination, cell polarity, tissue patterning and control of cell proliferation. Members of the Frizzled family of proteins serve as receptors for the Wnt signaling pathway. The founding member of this family was identified in Drosophila based on its role in tissue polarity in the adult cuticle and named for the disorganized appearance of bristle hairs on the mutant. Ten mammalian Frizzled genes have been identified to date. The predicted structure of Frizzled proteins is similar among all family members, containing a divergent N-terminal signal peptide, a highly conserved extracellular cysteine-rich domain, a variable-length linker region, a seven-pass transmembrane domain, and a variable-length C-terminal tail. One of the most conserved regions of the Frizzled proteins is the extracellular cysteine-rich domain (CRD) which spans approximately 120 amino acids (aa) and contains 10 invariant cysteines (1). Human and mouse Frizzled-2 are 100% identical at the aa level. Frizzled-2 expression is greater in embryonic than adult tissues, with heart, brain, lung, kidney and gut showing the highest levels (2). In addition, Frizzled-2 is expressed during migration and proliferation of neural crest cells that populate the heart and great arteries (3). However, Frizzled-2 may not be strictly involved in developmental processes, as its expression is also up-regulated in myofibroblasts during tissue repair after myocardial infarction (4). Frizzled-2 is implicated in the Wnt/Ca2+ pathway, a mechanism by which Wnt-5a signaling results in calcium release from intracellular stores and activation of PKC and calmodulin-dependent protein kinase (5). Secreted frizzled related proteins (sFRPs), antagonists of the Wnt pathway, have been shown to interact with both Wnt ligands and Frizzled receptors (6).

1. Wang, Y. et al. (1996) J. Biol. Chem 271:4468.
2. Malik, T. and R. Shivdasani (2000) Biochem. J. 349:829.
3. van Gijn, M. et al. (2001) Anat. Embryol. 203:185.
4. Toyofuku, T. et al. (2000) J. Cell Biol. 150:225.
5. Kuhl, M. et al. (2000) Trends Genet. 16: 279.
6. Bafico, A. et al. (1999) J. Biol. Chem. 274:16180.

Publications for Frizzled-2 (1307-FZ)(5)

Publications using 1307-FZ

• S Tian, X Xiong, J Zeng, S Wang, BJ Tremblay, P Chen, B Chen, M Liu, P Chen, K Sheng, D Zeve, W Qi, DT Breault, C Rodríguez, R Gerhard, R Jin, AC Doxey, M Dong Identification of TFPI as a receptor reveals recombination-driven receptor switching in Clostridioides difficile toxin B variants Nature Communications, 2022-11-09;13(1):6786. 2022-11-09 [PMID: 36351897] (Reference Standard, Human)
• R Chidiac, M Abedin, G Macleod, A Yang, PE Thibeault, LL Blazer, JJ Adams, L Zhang, H Roehrich, HN Jo, S Seshagiri, SS Sidhu, HJ Junge, S Angers A Norrin/Wnt surrogate antibody stimulates endothelial cell barrier function and rescues retinopathy Embo Molecular Medicine, 2021-06-09;0(0):e13977. 2021-06-09 [PMID: 34105895] (Bioassay, Human)
• Z Peng, R Simeon, SB Mitchell, J Zhang, H Feng, Z Chen Designed Ankyrin Repeat Protein (DARPin) Neutralizers of TcdB from Clostridium difficile Ribotype 027 mSphere, 2019-10-02;4(5):. 2019-10-02 [PMID: 31578248] (ELISA (Capture), Human)
• Wnt-5a/Frizzled9 receptor signaling through the G?o/G?? complex regulates dendritic spine formation J Biol Chem, 2016-07-11;0(0):. 2016-07-11 [PMID: 27402827] (Bioassay)
• Lindsley RC, Gill JG, Kyba M, Murphy TL, Murphy KM Canonical Wnt signaling is required for development of embryonic stem cell-derived mesoderm. Development, 2006-08-30;133(19):3787-96. 2006-08-30 [PMID: 16943279] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: FZD2
• Uniprot:
  • Q9JIP6()