Recombinant Human/Mouse Frizzled-2 Fc Chimera Protein, CF

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications Binding Activity
Format
Carrier-Free

Order Details

Recombinant Human/Mouse Frizzled-2 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its ability to bind biotinylated Wnt-5a in a functional ELISA with an estimated Kd <5 nM.
Source
Mouse myeloma cell line, NS0-derived Frizzled-2 protein
Human/Mouse Frizzled-2
(Gln29-Leu168)
Accession # Q9JIP6
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
No results obtained: Gln29 predicted
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
FZD2
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Binding Activity
Theoretical MW
42.3 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
50-55 kDa, reducing conditions
Publications
Read Publications using
1307-FZ in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human/Mouse Frizzled-2 Fc Chimera Protein, CF

  • frizzled (Drosophila) homolog 2
  • frizzled homolog 2 (Drosophila)
  • Frizzled2
  • Frizzled-2
  • Fz2
  • fz-2
  • FZD2
  • fzE2
  • hFz2

Background

Wnt signaling is involved in variety of developmental processes including cell fate determination, cell polarity, tissue patterning and control of cell proliferation. Members of the Frizzled family of proteins serve as receptors for the Wnt signaling pathway. The founding member of this family was identified in Drosophila based on its role in tissue polarity in the adult cuticle and named for the disorganized appearance of bristle hairs on the mutant. Ten mammalian Frizzled genes have been identified to date. The predicted structure of Frizzled proteins is similar among all family members, containing a divergent N-terminal signal peptide, a highly conserved extracellular cysteine-rich domain, a variable-length linker region, a seven-pass transmembrane domain, and a variable-length C-terminal tail. One of the most conserved regions of the Frizzled proteins is the extracellular cysteine-rich domain (CRD) which spans approximately 120 amino acids (aa) and contains 10 invariant cysteines (1). Human and mouse Frizzled-2 are 100% identical at the aa level. Frizzled-2 expression is greater in embryonic than adult tissues, with heart, brain, lung, kidney and gut showing the highest levels (2). In addition, Frizzled-2 is expressed during migration and proliferation of neural crest cells that populate the heart and great arteries (3). However, Frizzled-2 may not be strictly involved in developmental processes, as its expression is also up-regulated in myofibroblasts during tissue repair after myocardial infarction (4). Frizzled-2 is implicated in the Wnt/Ca2+ pathway, a mechanism by which Wnt-5a signaling results in calcium release from intracellular stores and activation of PKC and calmodulin-dependent protein kinase (5). Secreted frizzled related proteins (sFRPs), antagonists of the Wnt pathway, have been shown to interact with both Wnt ligands and Frizzled receptors (6).

  1. Wang, Y. et al. (1996) J. Biol. Chem 271:4468.
  2. Malik, T. and R. Shivdasani (2000) Biochem. J. 349:829.
  3. van Gijn, M. et al. (2001) Anat. Embryol. 203:185.
  4. Toyofuku, T. et al. (2000) J. Cell Biol. 150:225.
  5. Kuhl, M. et al. (2000) Trends Genet. 16: 279.
  6. Bafico, A. et al. (1999) J. Biol. Chem. 274:16180.

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Publications for Frizzled-2 (1307-FZ)(4)

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Bioinformatics

Gene Symbol FZD2
Uniprot