Recombinant Human MEPE/OF45 Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human MEPE/OF45 Protein, CF Summary

Details of Functionality
Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. rhMEPE, immobilized at 2 µg/mL (100 µL/well), can induce more than 60% of ATDC5 cell adhesion.
Source
Mouse myeloma cell line, NS0-derived human MEPE/OF45 protein
Pro18-Asp525 & Phe20-Asp525 & Gln21-Asp525, all with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Pro18, Phe20 & Gln21
Protein/Peptide Type
Recombinant Proteins
Gene
MEPE
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
82 kDa, reducing conditions
Publications
Read Publications using
3140-ME in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human MEPE/OF45 Protein, CF

  • extracellular phosphoglycoprotein with ASARM motif (bone)
  • matrix extracellular phosphoglycoprotein
  • MEPE
  • OF45
  • Osteoregulin

Background

MEPE (matrix extracellular phosphoglycoprotein), known as OF45 in mouse and rat, is a 55 kDa member of the SIBLING protein family. MEPE is primarily expressed in bone and dentin, where it regulates the mineralization of those tissues (1 - 3). The human MEPE cDNA encodes a 525 amino acid (aa) precursor that includes a 17 aa signal sequence. MEPE contains multiple consensus sites for post-translational modifications, including N-linked glycosylation, N-myristoylation, glycosaminoglycan attachment, and phosphorylation by a variety of kinases. MEPE also contains several putative proteolytic cleavage sites and one integrin-binding RGD motif (3, 4). There is therefore considerable potential for post-translational regulation of MEPE function and its degradation products. MEPE is secreted by osteoblasts and dental pulp stem cells during the mineralization process (5 - 7) and also by nonmineralizing tissues including epithelial cells in the renal proximal tubule and salivary duct (8, 9). MEPE has an inhibitory function in bone formation, (5) although a peptide corresponding to aa 242 - 264 stimulates new bone formation and the proliferation of osteoblasts and dental pulp stem cells (10, 11). MEPE contains one C-terminal ASARM motif common to SIBLING proteins. Similar to intact MEPE, the ASARM peptide inhibits bone mineralization and plays a central role in the phosphaturia and reduced mineralization of X-linked hypophosphatemic rickets (HYP) and tumor-induced osteomalacia (TIO) (12, 13). The zinc metalloprotease Phex binds directly to MEPE via the ASARM motif and prevents ASARM cleavage (13, 14). Multiple inactivating mutations in Phex are found in HYP and TIO and result in the increased liberation of ASARM peptide (15). Both MEPE and ASARM peptide are elevated in these disorders of mineralization and phosphate metabolism (12).

  1. Fisher, L.W. and N.S. Fedarko (2003) Connect. Tiss. Res. 44:33.
  2. Quarles, L.D. (2003) Am. J. Physiol. 285:E1.
  3. Qin, C. et al. (2004) Crit. Rev. Oral Biol. Med. 15:126.
  4. Rowe, P.S.N. et al. (2000) Genomics 67:54.
  5. Gowen, L.C. et al. (2003) J. Biol. Chem. 278:1998.
  6. Siggelkow, H. et al. (2004) Bone 35:570.
  7. Liu, H. et al. (2005) Arch. Oral Biol. 50:923.
  8. Ogbureke, K.U.E. and Fisher, L.W. (2005) Kidney Int. 68:155.
  9. Ogbureke, K.U.E. and L.W. Fisher (2004) J. Dent. Res. 83:664.
  10. Hayashibara, T. et al. (2004) J. Bone Miner. Res. 19:455.
  11. Liu, H. et al. (2004) J. Dent. Res. 83:496.
  12. Bresler, D. et al. (2004) J. Endocrinol. 183:R1.
  13. Rowe, P.S.N. et al. (2005) Bone 36:33.
  14. Guo, R. et al. (2002) Biochem. Biophys. Res. Commun. 297:38.
  15. Rowe, P.S. (2004) Crit. Rev. Oral Biol. Med.15:264.

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Publications for MEPE/OF45 (3140-ME)(2)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: Bioassay.


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Bioinformatics

Gene Symbol MEPE
Uniprot