Recombinant Human Melanotransferrin/CD228 His Protein, CF

2 μg/lane of Recombinant Human Melanotransferrin/CD228 His-tag Protein (Catalog # 10696-MT) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 84-92 kDa and 75-85 kDa, respectively.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Melanotransferrin/CD228 His Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. Recombinant Human Melanotransferrin/CD228 His-tag (Catalog # 10696-MT) binds Human Melanotransferrin/CD228 Antibody (Catalog # MAB81751) with an ED₅₀ of < 400 ng/mL.
• Source: Human embryonic kidney cell, HEK293-derived human Melanotransferrin/CD228 protein
  Gly20-Gly711, with a C-terminal 6-His tag
• Accession #: NP_005920.2
• N-terminal Sequence: Gly20
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 76 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 84-92 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 µg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Melanotransferrin/CD228 His Protein, CF

• antigen p97 (melanoma associated) identified by monoclonal antibodies 133.2 and96.5
• CD228 antigen
• CD228
• FLJ38863
• MAP97
• MAP97Melanoma-associated antigen p97
• Melanotransferrin
• membrane-bound transferrin-like protein
• MFI2
• MGC4856
• MTF
• MTF1
• p97

Background

Melanotransferrin (MTF), also known as CD228, P97, MELTF, or MFI2, is a member of the transferrin superfamily that binds to a single ferric iron with high affinity (1). It was originally identified as a membrane-bound protein with a coding region of 2217 bases encoding 738 amino acids (aa) including five Fe-binding residues, the consensus thermolysin metalloprotease sites, and a glycosyl-phosphatidylinositol (GPI) anchor (2). A secreted form of MTF (sMTF) has also been reported (2, 3). Human MTF shares 86% aa sequence identity with mouse and rat MTF. MTF expression is low in normal tissues but high in tumor and embryonic tissues. In melanoma MTF is associated with tumor metastasis and angiogenesis and upregulated in lung cancer tissue and cell lines (3). In the brain MTF is expressed in capillary endothelium but also in the reactive microglia associated with senile plaques in Alzheimer's disease (AD) (4). sMTF has been reported to be increased in patients with AD or arthritis and may modulate angiogenesis, cell migration, and plasminogen activation (5).

1. Baker, E.N. et al. (1992) FEBS Lett. 298:215.
2. Sekyere, E. & D.R. Richardson (2000) FEBS Lett. 483:11.
3. Lei, Y. et al. (2020) Cell Death Dis. 11:933.
4. Jefferies, W.A. et al. (1996) Brain Res. 712:122.
5. Paluncic, J. et al. (2016) Biochim. Biophy. Acta. 1863:770.

Bioinformatics

• Uniprot:
  • NP_005920.2()