Recombinant Human LYPD8 Fc Chimera Protein, CF

When Recombinant Human LYPD8 Fc Chimera is coated at 2 µg/ml, Recombinant Human Galectin‑3 (Catalog # 1154-GA) binds with a typical ED50 of 90-540 ng/mL.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human LYPD8 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human LYPD8 Fc Chimera is coated at 2 µg/mL, Recombinant Human Galectin‑3 (Catalog # 1154-GA) binds with a typical ED₅₀ = 90-540 ng/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived human LYPD8 protein
  

  Human LYPD8 (Leu20-Asn215) Accession # Q6UX82	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: Q6UX82
• N-terminal Sequence: Leu20
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: LYPD8
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 48 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 85-100 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human LYPD8 Fc Chimera Protein, CF

• LYPD8

Background

Ly6/PLAUR domain containing 8 (LYPD8), is a GPI-linked protein with structural similarity to the urokinase-type plasminogen activator receptor (uPAR) (1). Mature human LYPD8 contains one uPAR/Ly6 domain and a Ser/Thr/Pro-rich (STP) region that may serve as a target for protease mediated shedding as has been shown for the related C4.4A/LYPD3 molecule (2, 3). Mature human LYPD8 shares 40% amino acid sequence identity with mouse and rat LYPD8.

1. Kong, H.K. and J.H. Park (2012) BMB Rep. 45:595.
2. Hansen, L.V. et al. (2004) Biochem. J. 380:845.
3. Esselens, C.W. et al. (2008) Biol. Chem. 389:1075.

Bioinformatics

• Gene Symbol: LYPD8
• Uniprot:
  • Q6UX82()