Recombinant Human LRP-1 Cluster IV Fc Chimera Protein, CF

• Reactivity: Hu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Human LRP-1 Cluster IV Fc Chimera Protein, CF Summary

• Additional Information: This item is in process of being discontinued.
• Details of Functionality: Measured by its binding ability in a functional ELISA. In a Human IgG Fc Antibody (Catalog # G-102-C) coated plate, Recombinant Human LRP‑1 Cluster IV Fc Chimera binds Recombinant Human LRPAP (Catalog # 4296-LR) with an ED₅₀ of 0.25‑1.25 ng/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived human LRP-1 Cluster IV protein
  

  Human LRP1-C4 (Ser3332-Asp3779) Accession # Q07954	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: Q07954
• N-terminal Sequence: Ser3332
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: LRP1
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 76.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 110-120 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human LRP-1 Cluster IV Fc Chimera Protein, CF

• LRP1 Cluster IV
• LRP-1 Cluster IV

Background

LDL receptor-related protein 1 (LRP-1), also known as CD91 and the alpha 2-macroglobulin receptor, is a type I membrane protein in the LDL receptor superfamily. It is expressed on neurons, hepatocytes, adipocytes, vascular smooth muscle cells, fibroblasts, keratinocytes, macrophages, and megakaryocytes. LRP-1 is important for the clearance of a large number of circulating molecules involved in fatty acid metabolism and complexes of serine proteases with their inhibitors (1-4). LRP-1 also associates directly or through intracellular scaffold proteins with other membrane associated proteins on the same cell. This allows LRP-1 to modulate the activity or internalization of PDGF R beta , NMDA receptor subunits, TGF-beta receptors, Frizzled-1, various integrins, and the prion protein PrP^c. Human LRP-1 is an N-glycosylated and sialylated molecule that is cleaved in the Golgi to produce an 85 kDa transmembrane beta  chain and a 515 kDa alpha  chain that associates noncovalently with the beta  chain but does not itself cross the membrane (11, 12). The alpha chain of LRP-1 contains 31 LDLR class A repeats, 34 LDLR class B repeats, and 22 EGF-like repeats (13). The LDLR domains are clustered in four regions throughout the protein (13). Cluster IV (aa 3332-3779) contains eleven LDLR class A repeats (14). Within this region, human LRP-1 shares 99% aa sequence identity with mouse and rat LRP-1. A shed soluble form of LRP-1 circulates in the serum and retains ligand binding properties (15). Cluster IV contains binding sites for Apolipoprotein E, LPL, and LRPAP/RAP,  alpha 2-macroglobulin, Coagulation Factor VIII light chain, Lactoferrin, PAI-1, tPA-PAI-1 complexes, Pro-uPA, and TFPI (14).

1. Lillis, A.P. et al. (2008) Physiol. Rev. 88:887.
2. Galliano, M.-F. et al. (2008) PloS ONE 3:e2729.
3. Bouchard, B.A. et al. (2007) J. Thromb. Haemost. 6:638.
4. Sendra, J. et al. (2008) Cardiovasc. Res. 78:581.
5. Takayama, Y. et al. (2005) J. Biol. Chem. 280:18504.
6. Martin, A.M. et al. (2008) J. Biol. Chem. 283:12004.
7. Cabello-Verugio, C. and E. Brandan (2007) J. Biol. Chem. 282:18842.
8. Zilberberg, A. et al. (2004) J. Biol. Chem. 279:17535.
9. Taylor, D.R. and N.M. Hooper (2007) Biochem. J. 402:17.
10. Parkyn, C.J. et al. (2007) J. Cell Sci. 121:773.
11. Herz, J. et al. (1990) EMBO J. 9:1769.
12. Strickland, D.K. et al. (1990) J. Biol. Chem. 265:17401.
13. Herz, J. et al. (1988) EMBO J. 7:4119.
14. Neels, J.G. et al. (1999) J. Biol. Chem. 274:31305.
15. Quinn, K.A. et al. (1999) Exp. Cell Res. 251:433.

Publications for LRP-1 Cluster IV (5395-L4)(6)

Publications using 5395-L4

• K Sakamoto Generation of KS-487 as a novel LRP1-binding cyclic peptide with higher affinity, higher stability and BBB permeability Biochemistry and Biophysics Reports, 2022-10-08;32(0):101367. 2022-10-08 [PMID: 36237444] (ELISA Capture, N/A)
• K Sakamoto, T Shinohara, Y Adachi, T Asami, T Ohtaki A novel LRP1-binding peptide L57 that crosses the blood brain barrier Biochem Biophys Rep, 2017-08-12;12(0):135-139. 2017-08-12 [PMID: 29090274] (Bioassay, Human)
• Dudley K Strickland Evidence that factor VIII forms a bivalent complex with the LDL receptor-related protein 1 (LRP1): Identification of cluster IV on LRP1 as the major binding site J. Biol. Chem., 2016-10-29;0(0):. 2016-10-29 [PMID: 27794518] (Bioassay)
• Thevenard J, Verzeaux L, Devy J, Etique N, Jeanne A, Schneider C, Hachet C, Ferracci G, David M, Martiny L, Charpentier E, Khrestchatisky M, Rivera S, Dedieu S, Emonard H Low-density lipoprotein receptor-related protein-1 mediates endocytic clearance of tissue inhibitor of metalloproteinases-1 and promotes its cytokine-like activities. PLoS ONE, 2014-07-30;9(7):e103839. 2014-07-30 [PMID: 25075518] (Bioassay)
• Schorch B, Song S, van Diemen F, Bock H, May P, Herz J, Brummelkamp T, Papatheodorou P, Aktories K LRP1 is a receptor for Clostridium perfringens TpeL toxin indicating a two-receptor model of clostridial glycosylating toxins. Proc Natl Acad Sci U S A, 2014-04-15;111(17):6431-6. 2014-04-15 [PMID: 24737893] (Enzyme Assay)
• Ichimura A, Matsumoto S, Suzuki S, Dan T, Yamaki S, Sato Y, Kiyomoto H, Ishii N, Okada K, Matsuo O, Hou F, Vaughan D, van Ypersele de Strihou C, Miyata T A small molecule inhibitor to plasminogen activator inhibitor 1 inhibits macrophage migration. Arterioscler Thromb Vasc Biol, 2013-03-07;33(5):935-42. 2013-03-07 [PMID: 23471233] (Bioassay)

Bioinformatics

• Gene Symbol: LRP1
• Uniprot:
  • Q07954(Human)