Recombinant Human Lgr6 Fc Chimera Protein, CF

Recombinant Human Lgr6 induces Topflashreporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is2.5-12.5 µg/mL in the presence of 10 ng/mL Recombinant Mouse Wnt-3a (Catalog # 1324-WN).

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Lgr6 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED₅₀ for this effect is 2.5-12.5 μg/mL in the presence of 10 ng/mL Recombinant Mouse Wnt‑3a (Catalog # 1324-WN).
• Source: Chinese Hamster Ovary cell line, CHO-derived human Lgr6 protein
  

  Human Lgr6 (Ala25-Ala567) Accession # Q9HBX8	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: Q9HBX8
• N-terminal Sequence: Ala25
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: LGR6
• Purity: >80%, by SDS-PAGE with silver staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 87 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 90-108 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >80%, by SDS-PAGE with silver staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Lgr6 Fc Chimera Protein, CF

• FLJ14471
• gonadotropin receptor
• GPCR
• leucine-rich repeat containing G protein-coupled receptor 6
• leucine-rich repeat-containing G protein-coupled receptor 6
• leucine-rich repeat-containing G-protein coupled receptor 6
• Lgr6
• VTS20631

Background

Lgr6 (leucine‑rich repeat G protein‑coupled receptor 6) is an approximately 100 kDa 7‑transmembrane glycoprotein in the Lgr family of cell surface receptors. Lgrs has been identified as a marker for specific types of stem cells in tissues that are subject to constant self-renewal such as skin, gastrointestinal tract, and lungs (1, 2). The 544 amino acid (aa) N-terminal extracellular domain (ECD) of mature human Lgr6 contains 15 LRRs flanked by N-terminal and C-and C-terminal LRR domains. The ECD is followed by a 7-TM region and a 137 aa C-terminal cytoplasmic domain (3). Within the N-terminal ECD, human Lgr6 shares 92% and 93% aa sequence identity with mouse and rat Lgr6, respectively. Alternative splicing generates an additional isoform with a substitution for the LRR N-terminal domain and a deletion of LRR3-6. Lgr6 is expressed on hair follicle stem cells that give rise to sebaceous glands, interfollicular epidermis, and hair follicles (4-6). It is also expressed on alveolar stem cells that differentiate into lung epithelium (7). It is often up-regulated in human gastric cancers (8). Lgr6 binds to R‑Spondins 1-4 and can enhance Wnt/beta-Catenin signaling through phosphorylation of the Wnt co-receptor LRP6 (9, 10).

1. de Lau, W. et al. (2014) Genes Dev. 28:305.
2. Leushacke, M. and N. Barker (2012) Oncogene 31:3009.
3. Hsu, S.Y. et al. (2000) Mol. Endocrinol. 14:1257.
4. Snippert, H.J. et al. (2010) Science 327:1385.
5. Liao, X.-H. and H. Nguyen (2014) Exp. Dermatol. 23:195.
6. Nath, M. et al. (2011) Cell Tissue Res. 344:435.
7. Oeztuerk-Winder, F. et al. (2012) EMBO J. 31:3431.
8. Steffen, J.S. et al. (2012) Virchows Arch. 461:355.
9. Gong, X. et al. (2012) PLoS ONE 7:e37137.
10. de Lau, W. et al. (2011) Nature 476:293.

Bioinformatics

• Gene Symbol: LGR6
• Uniprot:
  • Q9HBX8()