Recombinant Human Lefty-A Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human Lefty-A Protein Summary

Details of Functionality
Measured by its ability to induce cell death using Mv1Lu mink lung epithelial cells. The ED50 for this effect is 0.5-3 µg/mL.
Source
Mouse myeloma cell line, NS0-derived human Lefty-A protein
    Mouse Lefty-1 Propeptide
    (Leu22-Arg77)
Accession # Q64280
HHHHHH
Human Lefty-A
(Phe78-Pro366) 
Accession # O00292       
N-terminus
C-terminus
Accession #
N-terminal Sequence
Leu22
Protein/Peptide Type
Recombinant Proteins
Gene
LEFTY2
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
44 kDa, 38 kDa and 34 kDa, reducing conditions
Publications
Read Publications using
746-LF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 500 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Lefty-A Protein

  • EBAFTGF-beta-4
  • endometrial bleeding associated factor
  • Endometrial bleeding-associated factor
  • Lefta
  • LEFTAProtein lefty-A
  • left-right determination factor 2
  • Left-right determination factor A
  • LEFTY2
  • LeftyA
  • Lefty-A
  • LEFTYATransforming growth factor beta-4
  • MGC46222
  • Protein lefty-2
  • TGFB4endometrial bleeding associated factor (left-right determination, factor A;transforming growth factor beta superfamily)

Background

Lefty was first identified in a screen for undifferentiated cell-specific cDNAs from the P19 mouse embryonal carcinoma cells. Its mRNA expression on the left side of the developing embryo earned the name “Lefty”. The human orthologue was initially identified as Ebaf, Endometrial Bleeding-Associated Factor. Two genes exist in mouse (Lefty-1 and Lefty-2) and two in humans (Lefty-A and Lefty-B). By amino acid sequence, human Lefty-A and -B are more similar to each other (96%) than to either Lefty-1 or -2 in the mouse (81 - 82% identical). Lefty contains the six cysteine residues that are conserved among TGF-beta related proteins and that are necessary to form the cysteine-knot structure. However, Lefty is distinct from other family members in that it has two RXXR cleavage sites, a longer carboxy terminal sequence, and it lacks the cysteine residue required for intermolecular disulfide linkage. Thus, mature forms of Lefty are larger than mature forms of other TGF-beta -related proteins.

Lefty homologues have been identified in other vertebrate organisms including chick, frog, and zebrafish. Although the amino acid sequence identity is not well conserved among vertebrate species, the expression pattern of Lefty on the left side is well conserved. Furthermore its function in patterning left-right asymmetry of the developing organ systems such as the heart and lung is consistent in all vertebrate species examined. Lefty acts as an antagonist to Nodal signaling, potentially by competing for binding to a common receptor.

  1. Meno, C. et al. (1996) Nature 381:151.
  2. Kosaki, K. et al. (1999) Am. J. Hum. Genet. 64:712.
  3. Schier, A.F. and M.M. Shen, (1999) Nature 403:385.
  4. Branford, W.W. et al. (2000) Dev. Biol. 223:291.
  5. Tabibzadeh, S. et al. (2000) J. Clin. Endocr. Metab. 85:2526.
  6. Ulloa, L. et al. (2001) J. Biol. Chem. 276:21387.

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Publications for Lefty-A (746-LF)(12)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Bioassay.


Filter By Application
Bioassay
(12)
All Applications
Filter By Species
Human
(12)
All Species
Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using 746-LF Applications Species
N Zeng, T Okumura, M Alauddin, S Khozooei, J Rajaxavier, S Zhang, Y Singh, B Shi, SY Brucker, D Wallwiener, S Takeda, F Lang, MS Salker LEFTY2/endometrial�bleeding-associated factor up-regulates Na+�Coupled Glucose Transporter SGLT1 expression and Glycogen Accumulation in Endometrial Cancer Cells PLoS ONE, 2020;15(4):e0230044. 2020 [PMID: 32236143] (Bioassay, Human) Bioassay Human
ML Sprouse, T Welte, D Boral, HN Liu, W Yin, M Vishnoi, D Goswami-Se, L Li, G Pei, P Jia, IC Glitza-Oli, D Marchetti PMN-MDSCs Enhance CTC Metastatic Properties through Reciprocal Interactions via ROS/Notch/Nodal Signaling Int J Mol Sci, 2019;20(8):. 2019 [PMID: 31003475] (Bioassay, Human) Bioassay Human
A Jørgensen, J Macdonald, JE Nielsen, KR Kilcoyne, S Perlman, L Lundvall, L Langhoff T, K Juul Hare, H Frederikse, AM Andersson, NE Skakkebæk, A Juul, RM Sharpe, E Rajpert-De, RT Mitchell Nodal Signaling Regulates Germ Cell Development and Establishment of Seminiferous Cords in the Human Fetal Testis Cell Rep, 2018;25(7):1924-1937.e4. 2018 [PMID: 30428358] (Bioassay, Human) Bioassay Human
KC Chang, J Hertz, X Zhang, XL Jin, P Shaw, BA Derosa, JY Li, P Venugopala, DA Valenzuela, RD Patel, KR Russano, SA Alshamekh, C Sun, K Tenerelli, C Li, D Velmeshev, Y Cheng, TM Boyce, A Dreyfuss, MS Uddin, KJ Muller, DM Dykxhoorn, JL Goldberg Novel regulatory mechanisms for the SoxC transcriptional network required for visual pathway development J. Neurosci., 2017;0(0):. 2017 [PMID: 28411269] (Bioassay, Human) Bioassay Human
LEFTYA Activates the Epithelial Na+ Channel (ENaC) in Endometrial Cells via Serum and Glucocorticoid Inducible Kinase SGK1 Cell Physiol Biochem, 2016;39(4):1295-306. 2016 [PMID: 27606670] (Bioassay, Human) Bioassay Human
LeftyA decreases Actin Polymerization and Stiffness in Human Endometrial Cancer Cells Sci Rep, 2016;6(0):29370. 2016 [PMID: 27404958] (Bioassay, Human) Bioassay Human
Madhuri S Salker, Nicolas Schierbau, Nour Alowayed, Yogesh Singh, Andreas F Mack, Christos Stournara, Tilman E Schäffer, Florian Lang LeftyA decreases Actin Polymerization and Stiffness in Human Endometrial Cancer Cells. Scientific Reports, 2016;0(0):2045-2322. 2016 [PMID: 27404958] (Bioassay, Human) Bioassay Human
Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 2012;487(7408):505-9. 2012 [PMID: 22763448] (Bioassay, Human) Bioassay Human
Cavallari, C, Fonsato, V, Herrera, M B, Bruno, S, Tetta, C, Camussi, G Role of Lefty in the anti tumor activity of human adult liver stem cells. Oncogene, 2013;32(7):819-26. 2013 [PMID: 22469982] (Bioassay, Human) Bioassay Human
Salker MS, Christian M, Steel JH, Nautiyal J, Lavery S, Trew G, Webster Z, Al-Sabbagh M, Puchchakayala G, Foller M, Landles C, Sharkey AM, Quenby S, Aplin JD, Regan L, Lang F, Brosens JJ Deregulation of the serum- and glucocorticoid-inducible kinase SGK1 in the endometrium causes reproductive failure. Nat. Med., 2011;17(11):1509-13. 2011 [PMID: 22001908] (Bioassay, Human) Bioassay Human
Show All 12 Publications.

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Bioinformatics

Gene Symbol LEFTY2
Uniprot