Recombinant Human Jagged 1 His-tag Protein, CF

Immobilized Recombinant Human Jagged 1 His-tag (Catalog # 10957-JG) enhances Recombinant Human/Mouse/Rat BMP-2 (355-BM) induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is 0.750-4.50 µg/mL.

2 μg/lane of Recombinant Human Jagged 1 His-tag Protein (Catalog # 10957-JG) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 125-150 kDa.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Jagged 1 His-tag Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. Nobta, M. et al. (2005) J. Biol. Chem. 280:15842. The ED₅₀ for this effect is 0.750-4.50 μg/mL.
• Source: Human embryonic kidney cell, HEK293-derived human Jagged 1 protein
  Ser32-Ser1046, with a C-terminal 6-His tag
• Accession #: P78504.3
• N-terminal Sequence: Ser32
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 111 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 125-150 kDa, reducing conditions.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Jagged 1 His-tag Protein, CF

• AGS
• AHDMGC104644
• Alagille syndrome
• AWS
• CD339 antigen
• CD339
• HJ1
• JAG1
• Jagged 1
• Jagged1
• JAGL1
• protein jagged-1

Background

Jagged 1 is a 180 kDa type I transmembrane glycoprotein and member of the Delta-Serrate-Lag-2 (DSL) family of ligands that activate LIN12/Notch proteins. Human Jagged 1 is synthesized as a 1218 amino acid (aa) precursor that contains a 33 aa signal sequence, a 1034 aa extracellular domain (ECD), a 26 aa transmembrane segment, and a 125 aa cytoplasmic region. The ECD contains a DSL domain (aa 185-229), a cysteine-rich region, 15 EGF-like repeats, of which many bind calcium, and nine potential sites for N-linked glycosylation. This product is an N-terminal ECD fragment that includes DSL domain and the first 3 EGF-like domains. Mature human Jagged 1 is 97% and 96% aa identical to mature mouse and rat Jagged 1, respectively. Jagged 1 is widely expressed in adult and fetal tissues. Jagged-Notch signaling specifies cell fate, regulates pattern formation, defines boundaries between different cell types, and modulates cell proliferation and differentiation, especially during hematopoiesis, myogenesis, neurogenesis, and development of vasculature (1-8). Mutations in human Jagged 1 are the cause of Alagille syndrome, an autosomal-dominant disorder characterized by intrahepatic cholestasis and abnormalities of heart, eye, vertebrae, as well as characteristic facial appearance (9, 10).

1. Sainson, R.C. and A.L. Harris (2008) Angiogenesis 11:41.
2. Cordle, J. et al. (2008) Nat. Struct. Mol. Biol. 15:849.
3. Artavamis-Tsokanas, S. et al. (1999) Science 284:770.
4. Lai, E.C. (2004) Development 131:965.
5. Milner, L.A. et al. (1994) Blood 83:2057.
6. Nyfeler, Y. et al. (2005) EMBO J. 24:3504.
7. Linheng, L. et al. (1998) Immunity 8:43.
8. Iso, T. et al. (2003) Arterioscler. Thromb. Vasc. Biol. 23:543.
9. Oda, T. et al. (1997) Nat. Genet. 16:235.
10. Oda, T. et al. (1997) Genomics 43:376.

Bioinformatics

• Uniprot:
  • P78504.3()