Recombinant Human Jagged 1 Fc Chimera Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Jagged 1 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. Nobta, M. et al. (2005) J. Biol. Chem. 280:15842. The ED 50 for this effect is 0.5-2.0 μg/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived human Jagged 1 protein

  Human Jagged 1 (Ser32-Ser1046) Accession # P78504

  IEGRMD

  Human IgG 1 (Pro100-Lys330)

  N-terminus

  C-terminus

• Accession #: P78504
• N-terminal Sequence: Ser32
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: JAG1
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 137 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 170-180 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 3 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS and EDTA.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 200 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Jagged 1 Fc Chimera Protein, CF

• AGS
• AHDMGC104644
• Alagille syndrome
• AWS
• CD339 antigen
• CD339
• HJ1
• JAG1
• Jagged 1
• Jagged1
• JAGL1
• protein jagged-1

Background

Jagged 1 is a 180 kDa type I transmembrane glycoprotein and member of the Delta-Serrate-Lag-2 (DSL) family of ligands that activate LIN12/Notch proteins. Human Jagged 1 is synthesized as a 1218 amino acid (aa) precursor that contains a 33 aa signal sequence, a 1034 aa extracellular domain (ECD), a 26 aa transmembrane segment, and a 125 aa cytoplasmic region (SwissProt # P78504). The ECD contains a DSL domain (aa 185-229), a cysteine-rich region, 15 EGF-like repeats, of which many bind calcium, and nine potential sites for N-linked glycosylation. Mature human Jagged 1 is 97% and 96% aa identical to mature mouse and rat Jagged 1, respectively. Jagged 1 is widely expressed in adult and fetal tissues. Jagged-Notch signaling specifies cell fate, regulates pattern formation, defines boundaries between different cell types, and modulates cell proliferation and differentiation, especially during hematopoiesis, myogenesis, neurogenesis, and development of vasculature (1-8). Mutations in human Jagged 1 are the cause of Alagille syndrome, an autosomal-dominant disorder characterized by intrahepatic cholestasis and abnormalities of heart, eye, vertebrae, as well as characteristic facial appearance (9-10).

1. Sainson, R.C. and A.L. Harris (2008) Angiogenesis 11:41.
2. Cordle, J. et al. (2008) Nat. Struct. Mol. Biol. 15:849.
3. Artavamis-Tsokanas, S. et al. (1999) Science 284:770.
4. Lai, E.C. (2004) Development 131:965.
5. Milner, L.A. et al. (1994) Blood 83:2057.  
6. Nyfeler, Y. et al. (2005) EMBO J. 24:3504
7. Linheng, L. et al. (1998) Immunity 8:43.
8. Iso, T. et al. (2003) Arterioscler. Thromb. Vasc. Biol. 23:543.
9. Oda, T. et al. (1997) Nat. Genet. 16:235.
10. Oda, T. et al. (1997) Genomics 43:376.

Publications for Jagged 1 (1277-JG)(48)

Publications using 1277-JG

• Cho, S;Servián-Morilla, E;Garrido, VN;Rodriguez-Gonzalez, B;Yuan, Y;Cano, R;Rambhiya, AA;Darabi, R;Haltiwanger, RS;Paradas, C;Jafar-Nejad, H; The glycosyltransferase POGLUT1 regulates muscle stem cell development and maintenance in mice bioRxiv : the preprint server for biology 2024-11-25 [PMID: 39651163] (Bioassay, Mouse)
• Carolina, E;Kuse, Y;Okumura, A;Aoshima, K;Tadokoro, T;Matsumoto, S;Kanai, E;Okumura, T;Kasai, T;Yamabe, S;Nishikawa, Y;Yamaguchi, K;Furukawa, Y;Tanimizu, N;Taniguchi, H; Generation of human iPSC-derived 3D bile duct within liver organoid by incorporating human iPSC-derived blood vessel Nature communications 2024-08-28 [PMID: 39198465] (Bioassay, Human)
• Herrera, JL;Komatsu, M; Akt3 activation by R-Ras in an endothelial cell enforces quiescence and barrier stability of neighboring endothelial cells via Jagged1 Cell reports 2024-02-24 [PMID: 38402584] (Bioassay, Human)
• Jovanovic, VM;Weber, C;Slamecka, J;Ryu, S;Chu, PH;Sen, C;Inman, J;De Sousa, JF;Barnaeva, E;Hirst, M;Galbraith, D;Ormanoglu, P;Jethmalani, Y;Mercado, JC;Michael, S;Ward, ME;Simeonov, A;Voss, TC;Tristan, CA;Singeç, I; A defined roadmap of radial glia and astrocyte differentiation from human pluripotent stem cells Stem cell reports 2023-07-04 [PMID: 37451260] (Bioassay, Human)
• D Li, A Sun, L Zhang, Z Ding, F Yi, X Yang, Z Wang, X Chen, W Liu, S Liu, H Shen, M Miao, L Zhang, P Liu, Y Liu, S Su, H Huang, C Huang, Z Hu, H Zhang, X Zha, Y Liu Elevated ITGA5 facilitates hyperactivated mTORC1-mediated progression of laryngeal squamous cell carcinoma via upregulation of EFNB2 Theranostics, 2022-10-24;12(17):7431-7449. 2022-10-24 [PMID: 36438491] (Bioassay, Human)
• A Chansaenro, C Kornsuthis, S Roytrakul, S Phothichai, S Rochanavib, BPJ Fournier, SS Srithanyar, N Nowwarote, T Osathanon Indirect Immobilised Jagged-1 Enhances Matrisome Proteins Associated with Osteogenic Differentiation of Human Dental Pulp Stem Cells: A Proteomic Study International Journal of Molecular Sciences, 2022-11-11;23(22):. 2022-11-11 [PMID: 36430375] (Bioassay, Human)
• Maša Filipović, Darja Flegar, Alan Šućur, Dino Šisl, Inga Kavazović, Mariastefania Antica et al. Inhibition of Notch Signaling Stimulates Osteoclastogenesis From the Common Trilineage Progenitor Under Inflammatory Conditions Frontiers in Immunology 2022-07-05 [PMID: 35865541] (Bioassay, Mouse)
• C Kornsuthis, A Chansaenro, J Manokawinc, KA Tompkins, N Pirarat, T Osathanon Non-canonical Wnt signaling participates in Jagged1-induced osteo/odontogenic differentiation in human dental pulp stem cells Scientific Reports, 2022-05-09;12(1):7583. 2022-05-09 [PMID: 35534526] (Bioassay, Human)
• FJM Roos, GS van Tiende, H Wu, I Bordeu, D Vinke, LM Albarinos, K Monfils, S Niesten, R Smits, J Willemse, O Rosmark, G Westergren, DJ Kunz, M de Wit, PJ French, L Vallier, JNM IJzermans, R Bartfai, H Marks, BD Simons, ME van Royen, MMA Verstegen, LJW van der La Human branching cholangiocyte organoids recapitulate functional bile duct formation Cell Stem Cell, 2022-05-05;29(5):776-794.e13. 2022-05-05 [PMID: 35523140] (Bioassay, Human)
• Shogo Tamura, Masato Mukaide, Yumi Katsuragi, Wataru Fujii, Koya Odaira, Nobuaki Suzuki et al. Periosteum-derived podoplanin-expressing stromal cells regulate nascent vascularization during epiphyseal marrow development Journal of Biological Chemistry 2022-05-01 [PMID: 35304101] (Bioassay, Mouse)
• K Zohorsky, S Lin, K Mequanint Immobilization of Jagged1 Enhances Vascular Smooth Muscle Cells Maturation by Activating the Notch Pathway Cells, 2021-08-14;10(8):. 2021-08-14 [PMID: 34440858] (Bioassay, Human)
• Y Wang, J Chang, ZQ Wang, Y Li Sirt3 promotes the autophagy of HK?2 human proximal tubular epithelial cells via the inhibition of Notch?1/Hes?1 signaling Molecular Medicine Reports, 2021-07-19;24(3):. 2021-07-19 [PMID: 34278469] (Bioassay, Human)
• Z Zhao, X Shen, D Zhang, H Xiao, H Kong, B Yang, L Yang miR-153 enhances the therapeutic effect of radiotherapy by targeting JAG1 in pancreatic cancer cells Oncology Letters, 2021-02-17;21(4):300. 2021-02-17 [PMID: 33732376] (Bioassay, Human)
• S Shimizu, K Yoshioka, S Aki, Y Takuwa Class II phosphatidylinositol 3-kinase-C2&amp;alpha is essential for Notch signaling by regulating the endocytosis of &amp;gamma-secretase in endothelial cells Scientific Reports, 2021-03-04;11(1):5199. 2021-03-04 [PMID: 33664344] (Bioassay, Human)
• K Uchimura, H Wu, Y Yoshimura, BD Humphreys Human Pluripotent Stem Cell-Derived Kidney Organoids with Improved Collecting Duct Maturation and Injury Modeling Cell Reports, 2020-12-15;33(11):108514. 2020-12-15 [PMID: 33326782] (Bioassay, Human)
• S Kakuda, RK Lopilato, A Ito, RS Haltiwange Canonical Notch ligands and Fringes have distinct effects on NOTCH1 and NOTCH2 J. Biol. Chem., 2020-08-19;0(0):. 2020-08-19 [PMID: 32820046] (Bioassay, Human)
• J Manokawinc, P Sumrejkanc, L Boonprakon, P Pavasant, H Egusa, T Osathanon NOTCH2 participates in Jagged1-induced osteogenic differentiation in human periodontal ligament cells Sci Rep, 2020-08-07;10(1):13329. 2020-08-07 [PMID: 32770090] (Bioassay, Human)
• J Fan, W Shen, SR Lee, AE Mathai, R Zhang, G Xu, MC Gillies Targeting the Notch and TGF-&amp;beta signaling pathways to prevent retinal fibrosis in vitro and in vivo Theranostics, 2020-06-29;10(18):7956-7973. 2020-06-29 [PMID: 32724452] (Bioassay, Human)
• K Wei, I Korsunsky, JL Marshall, A Gao, GFM Watts, T Major, AP Croft, J Watts, PE Blazar, JK Lange, TS Thornhill, A Filer, K Raza, LT Donlin, CW Siebel, CD Buckley, S Raychaudhu, MB Brenner Notch signalling drives synovial fibroblast identity and arthritis pathology Nature, 2020-04-22;582(7811):259-264. 2020-04-22 [PMID: 32499639] (Bioassay, Human)
• MR Batista, P Diniz, A Torres, D Murta, L Lopes-da-C, E Silva Notch signaling in mouse blastocyst development and hatching BMC Dev. Biol., 2020-06-02;20(1):9. 2020-06-02 [PMID: 32482162] (Bioassay, Mouse)
• SH Chung, W Shen, KC Davidson, A Pébay, RCB Wong, B Yau, M Gillies Differentiation of Retinal Glial Cells From Human Embryonic Stem Cells by Promoting the Notch Signaling Pathway Front Cell Neurosci, 2019-12-03;13(0):527. 2019-12-03 [PMID: 31849614] (Bioassay, Human)
• M López-Guer, S Xargay-Tor, P Fuentes, J Roldán, B González-F, L Rosich, E Silkensted, MJ García-Leó, E Lee-Vergés, N Giménez, A Giró, M Aymerich, N Villamor, J Delgado, A López-Guil, XS Puente, E Campo, ML Toribio, D Colomer Specific NOTCH1 antibody targets DLL4-induced proliferation, migration, and angiogenesis in NOTCH1-mutated CLL cells Oncogene, 2019-10-15;0(0):. 2019-10-15 [PMID: 31616059] (Bioassay, Human)
• S Kannan, MJL Aitken, SM Herbrich, LS Golfman, MG Hall, DH Mak, JK Burks, G Song, M Konopleva, CG Mullighan, J Chandra, PA Zweidler-M Anti-Leukemia Effects of Notch-Mediated Inhibition of Oncogenic PLK1 in B-Cell Acute Lymphoblastic Leukemia Mol. Cancer Ther., 2019-06-21;0(0):. 2019-06-21 [PMID: 31227645] (Bioassay, Human)
• S Matsui, M Ochiai, K Yasuda, SI Mae, M Kotaka, T Toyoda, T Yamamoto, K Osafune Differentiation and isolation of iPSC-derived remodeling ductal plate-like cells by use of an AQP1-GFP reporter human iPSC line Stem Cell Res, 2019-01-31;35(0):101400. 2019-01-31 [PMID: 30735882] (Bioassay, Human)
• S Stephenson, MA Care, I Fan, A Zougman, DR Westhead, GM Doody, RM Tooze Growth Factor-like Gene Regulation Is Separable from Survival and Maturation in Antibody-Secreting Cells J. Immunol., 2019-01-14;202(4):1287-1300. 2019-01-14 [PMID: 30642980] (Bioassay, Human)
• R Schwentner, G Jug, MO Kauer, T Schnöller, P Waidhofer-, W Holter, C Hutter JAG2 signaling induces differentiation of CD14+ monocytes into Langerhans cell histiocytosis-like cells J. Leukoc. Biol., 2018-10-08;105(1):101-111. 2018-10-08 [PMID: 30296338] (Binding Assay, Human)
• SY Lee, F Long Notch signaling suppresses glucose metabolism in mesenchymal progenitors to restrict osteoblast differentiation J. Clin. Invest., 2018-11-12;0(0):. 2018-11-12 [PMID: 30284985] (Bioassay, Mouse)
• J Manokawinc, P Nattasit, T Thongngam, P Pavasant, KA Tompkins, H Egusa, T Osathanon RNA sequencing data of Notch ligand treated human dental pulp cells Data Brief, 2018-01-31;17(0):407-413. 2018-01-31 [PMID: 29876410] (Bioassay, Human)
• CJM Antila, V Rraklli, HA Blomster, KM Dahlström, TA Salminen, J Holmberg, L Sistonen, C Sahlgren Sumoylation of Notch1 represses its target gene expression during cell stress Cell Death Differ., 2018-01-05;0(0):. 2018-01-05 [PMID: 29305585] (Bioassay, Human)
• DW Youngstrom, R Senos, RL Zondervan, JD Brodeur, AR Lints, DR Young, TL Mitchell, ME Moore, MH Myers, WJ Tseng, KM Loomes, KD Hankenson Intraoperative delivery of the Notch ligand Jagged-1 regenerates appendicular and craniofacial bone defects NPJ Regen Med, 2017-12-15;2(0):32. 2017-12-15 [PMID: 29302365] (In Vivo, Mouse)
• T Gocha, BM Rao, R DasGupta Identification and characterization of a novel Sso7d scaffold-based binder against Notch1 Sci Rep, 2017-09-20;7(1):12021. 2017-09-20 [PMID: 28931897] (Bioassay, Human)
• X Hong, A Margariti, A Le Bras, L Jacquet, W Kong, Y Hu, Q Xu Transdifferentiated Human Vascular Smooth Muscle Cells are a New Potential Cell Source for Endothelial Regeneration Sci Rep, 2017-07-17;7(1):5590. 2017-07-17 [PMID: 28717251] (Bioassay, Human)
• Z Liu, C Li, X Meng, Y Bai, J Qi, J Wang, Q Zhou, W Zhang, X Zhang Hypoxia-inducible factor-l? mediates aggrecan and collagen ? expression via NOTCH1 signaling in nucleus pulposus cells during intervertebral disc degeneration Biochem. Biophys. Res. Commun., 2017-05-17;0(0):. 2017-05-17 [PMID: 28526405] (Bioassay, Human)
• M Ciria, NA García, I Ontoria-Ov, H González-K, R Carrero, JL De La Pomp, JA Montero, P Sepúlveda Mesenchymal Stem Cell Migration and Proliferation Are Mediated by Hypoxia-Inducible Factor-1? Upstream of Notch and SUMO Pathways Stem Cells Dev., 2017-05-18;0(0):. 2017-05-18 [PMID: 28520516] (Bioassay, Human)
• Jagged1 Instructs Macrophage Differentiation in Leprosy PLoS Pathog, 2016-08-17;12(8):e1005808. 2016-08-17 [PMID: 27532668] (Bioassay, Human)
• Rouch J, Scott A, Lei N, Solorzano-Vargas R, Wang J, Hanson E, Kobayashi M, Lewis M, Stelzner M, Dunn J, Eckmann L, Martin M Development of Functional Microfold (M) Cells from Intestinal Stem Cells in Primary Human Enteroids. PLoS ONE, 2016-01-28;11(1):e0148216. 2016-01-28 [PMID: 26820624] (Bioassay, Human)
• Procopio M, Laszlo C, Al Labban D, Kim D, Bordignon P, Jo S, Goruppi S, Menietti E, Ostano P, Ala U, Provero P, Hoetzenecker W, Neel V, Kilarski W, Swartz M, Brisken C, Lefort K, Dotto G Combined CSL and p53 downregulation promotes cancer-associated fibroblast activation. Nat Cell Biol, 2015-08-24;17(9):1193-204. 2015-08-24 [PMID: 26302407] (Bioassay, Human)
• Shao , Shan, Zhao , Xiaoai, Zhang , Xiaojin, Luo , Minna, Zuo , Xiaoxiao, Huang , Shangke, Wang , Ying, Gu , Shanzhi, Zhao , Xinhan Notch1 signaling regulates the epithelial-mesenchymal transition and invasion of breast cancer in a Slug-dependent manner. Mol Cancer, 2015-02-03;14(1):28. 2015-02-03 [PMID: 25645291] (Bioassay, Human)
• Ghiabi P, Jiang J, Pasquier J, Maleki M, Abu-Kaoud N, Halabi N, Guerrouahen B, Rafii S, Rafii A Breast cancer cells promote a notch-dependent mesenchymal phenotype in endothelial cells participating to a pro-tumoral niche. J Transl Med, 2015-01-27;13(0):27. 2015-01-27 [PMID: 25623554] (Bioassay, Human)
• Hirata , Naoya, Yamada , Shigeru, Shoda , Takuji, Kurihara , Masaaki, Sekino , Yuko, Kanda , Yasunari Sphingosine-1-phosphate promotes expansion of cancer stem cells via S1PR3 by a ligand-independent Notch activation. Nat Commun, 2014-09-25;5(0):4806. 2014-09-25 [PMID: 25254944] (Bioassay, Human)
• Ranga A, Gobaa S, Okawa Y, Mosiewicz K, Negro A, Lutolf M 3D niche microarrays for systems-level analyses of cell fate. Nat Commun, 2014-07-14;5(0):4324. 2014-07-14 [PMID: 25027775] (Bioassay)
• Guichet P, Guelfi S, Teigell M, Hoppe L, Bakalara N, Bauchet L, Duffau H, Lamszus K, Rothhut B, Hugnot J Notch1 stimulation induces a vascularization switch with pericyte-like cell differentiation of glioblastoma stem cells. Stem Cells, 2015-01-01;33(1):21-34. 2015-01-01 [PMID: 24898819] (Bioassay, Human)
• Zeng Q, Song R, Ao L, Xu D, Venardos N, Fullerton D, Meng X Augmented osteogenic responses in human aortic valve cells exposed to oxLDL and TLR4 agonist: a mechanistic role of Notch1 and NF-kappaB interaction. PLoS ONE, 2014-05-08;9(5):e95400. 2014-05-08 [PMID: 24810405] (Bioassay, Human)
• Osathanon T, Manokawinchoke J, Nowwarote N, Aguilar P, Palaga T, Pavasant P Notch signaling is involved in neurogenic commitment of human periodontal ligament-derived mesenchymal stem cells. Stem Cells Dev, 2013-02-12;22(8):1220-31. 2013-02-12 [PMID: 23379739] (Bioassay, Human)
• Sundlisaeter E, Edelmann R, Hol J, Sponheim J, Kuchler A, Weiss M, Udalova I, Midwood K, Kasprzycka M, Haraldsen G The alarmin IL-33 is a notch target in quiescent endothelial cells. Am J Pathol, 2012-07-16;181(3):1099-111. 2012-07-16 [PMID: 22809957] (Bioassay, Human)
• Lavoz C, Rodrigues-Diez R, Benito-Martin A, Rayego-Mateos S, Rodrigues-Diez RR, Alique M, Ortiz A, Mezzano S, Egido J, Ruiz-Ortega M Angiotensin II Contributes to Renal Fibrosis Independently of Notch Pathway Activation. PLoS ONE, 2012-07-09;7(7):e40490. 2012-07-09 [PMID: 22792351] (Bioassay, Mouse)
• Emdad L, D'Souza SL, Kothari HP, Qadeer ZA, Germano IM Efficient differentiation of human embryonic and induced pluripotent stem cells into functional astrocytes. Stem Cells Dev., 2011-07-26;21(3):404-10. 2011-07-26 [PMID: 21631388] (Bioassay, Human)
• Piggott K, Deng J, Warrington K, Younge B, Kubo JT, Desai M, Goronzy JJ, Weyand CM Blocking the NOTCH pathway inhibits vascular inflammation in large-vessel vasculitis. Circulation, 2011-01-10;123(3):309-18. 2011-01-10 [PMID: 21220737] (Bioassay, Human)

Bioinformatics

• Gene Symbol: JAG1
• Uniprot:
  • P78504(Human)