Recombinant Human Jagged 1 Fc Chimera Avi-tag Protein, CF

When Recombinant Human Notch‑1 Fc Chimera (3647-TK) is immobilized at 1.00 µg/mL (100 µL/well), Biotinylated Recombinant Human Jagged 1 Fc Chimera Avi-tag Protein (Catalog # AVI1277) binds with an ED50 of 0.500-2.50 µg/mL.

2 μg/lane of Biotinylated Recombinant Human Jagged 1 Fc Chimera Avi-tag Protein (Catalog # AVI1277) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 150-170 kDa and 300-340 kDa, respectively.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Jagged 1 Fc Chimera Avi-tag Protein, CF Summary

• Additional Information: Biotinylated
• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human Notch‑1 Fc Chimera (Catalog # 3647-TK) is immobilized at 1.00 µg/mL (100 µL/well), Biotinylated Recombinant Human Jagged 1 Fc Chimera Avi-tag Protein binds with an ED₅₀ of 0.500-2.50 µg/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived human Jagged 1 protein
  

  Human Jagged 1 (Ser32-Ser1046) Accession # P78504.3	IEGRMD	Human IgG1 (Pro100-Lys330)	Avi-tag
  N-terminus			C-terminus

• Accession #: P78504.3
• N-terminal Sequence: Ser32
• Structure / Form: Disulfide-linked homodimer
  Biotinylated via Avi-tag
• Protein/Peptide Type: Recombinant Proteins
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 139 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 150-170 kDa, under reducing conditions.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 250 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Jagged 1 Fc Chimera Avi-tag Protein, CF

• AGS
• AHDMGC104644
• Alagille syndrome
• AWS
• CD339 antigen
• CD339
• HJ1
• JAG1
• Jagged 1
• Jagged1
• JAGL1
• protein jagged-1

Background

Jagged 1 is a 180 kDa type I transmembrane glycoprotein and member of the Delta-Serrate-Lag-2 (DSL) family of ligands that activate LIN12/Notch proteins. Human Jagged 1 is synthesized as a 1218 amino acid (aa) precursor that contains a 33 aa signal sequence, a 1034 aa extracellular domain (ECD), a 26 aa transmembrane segment, and a 125 aa cytoplasmic region (SwissProt # P78504). The ECD contains a DSL domain (aa 185-229), a cysteine-rich region, 15 EGF-like repeats, of which many bind calcium, and nine potential sites for N-linked glycosylation. Mature human Jagged 1 is 97% and 96% aa identical to mature mouse and rat Jagged 1, respectively. Jagged 1 is widely expressed in adult and fetal tissues. Jagged-Notch signaling specifies cell fate, regulates pattern formation, defines boundaries between different cell types, and modulates cell proliferation and differentiation, especially during hematopoiesis, myogenesis, neurogenesis, and development of vasculature (1-8). Mutations in human Jagged 1 are the cause of Alagille syndrome, an autosomal-dominant disorder characterized by intrahepatic cholestasis and abnormalities of heart, eye, vertebrae, as well as characteristic facial appearance (9-10). Our Avi-tag Biotinylated Human Jagged 1 Fc Chimera protein features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide.  Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

1. Sainson, R.C. and A.L. Harris (2008) Angiogenesis 11:41.
2. Cordle, J. et al. (2008) Nat. Struct. Mol. Biol. 15:849.
3. Artavamis-Tsokanas, S. et al. (1999) Science 284:770.
4. Lai, E.C. (2004) Development 131:965.
5. Milner, L.A. et al. (1994) Blood 83:2057.  
6. Nyfeler, Y. et al. (2005) EMBO J. 24:3504
7. Linheng, L. et al. (1998) Immunity 8:43.
8. Iso, T. et al. (2003) Arterioscler. Thromb. Vasc. Biol. 23:543.
9. Oda, T. et al. (1997) Nat. Genet. 16:235.
10. Oda, T. et al. (1997) Genomics 43:376.

Bioinformatics

• Uniprot:
  • P78504.3()