Recombinant Human Integrin alpha 5 beta 1 Protein, CF

Product Discontinued

Recombinant Human Integrin alpha 5 beta 1 Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. Recombinant Human Integrin alpha 5 beta 1 binds Bovine Fibronectin (Catalog # 1030-FN) coated at 2 µg/mL with an apparent K_d <0.5 nM in the presence of 1 mM MnCl₂.
• Source: Chinese Hamster Ovary cell line, CHO-derived human Integrin alpha 5 beta 1 protein
  

  Human Integrin alpha 5 (Phe42-Tyr995) Accession # P08648	acidic tail
  Human Integrin beta 1 (Gln21-Asp728) Accession # P05556	basic tail
  N-terminus	C-terminus

• Accession #: P08648 (Integrin alpha 5) & P05556 (Integrin beta 1)
• N-terminal Sequence: Phe42 (Integrin alpha 5) & No results obtained: Gln21 predicted (Integrin beta 1)
• Protein/Peptide Type: Recombinant Proteins
• Gene: ITGA5
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 108 kDa ( alpha 5 subunit), 82.3 kDa ( beta 1 subunit).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 105-150 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Integrin alpha 5 beta 1 Protein, CF

• CD49 antigen-like family member E
• CD49e
• Fibronectin receptor subunit alpha
• fibronectin receptor, alpha polypeptide
• fibronectin receptor, alpha subunit
• FNRA
• Integrin alpha 5 beta 1
• integrin alpha-5
• Integrin alpha-F
• integrin, alpha 5 (fibronectin receptor, alpha polypeptide)
• ITGA5/SLC9B1 fusion
• very late activation protein 5, alpha subunit
• VLA-5
• VLA5A

Background

Integrin alpha 5/ beta 1, also known as VLA-5, is a widely expressed non-covalent heterodimer of a 160 kDa alpha 5 and a 130 kDa beta 1 Integrin subunit. alpha 5/ beta 1 functions in cell adhesion, migration, activation, and matrix fibrillogenesis (1, 2). The human Integrin alpha 5/CD49e cDNA encodes a 1049 amino acid (aa) precursor that includes a 41 aa signal sequence, a 954 aa extracellular domain (ECD), a 26 aa transmembrane segment, and a 28 aa cytoplasmic domain. The ECD contains seven FG-GAP repeats and nine internal disulfide bonds (3). Within the ECD, human alpha 5 shares 90% and 46% aa sequence identity with mouse alpha 5 and human alpha 8, respectively, and less than 30% aa sequence identitity with other human alpha chains. The human Integrin beta 1/CD29 cDNA encodes a 798 aa precursor that includes a 20 aa signal sequence, a 708 aa ECD, a 23 aa transmembrane segment, and a 47 aa cytoplasmic domain. The ECD contains one vWF-A domain, four Cys-rich repeats, and 29 internal disulfide bonds (3). Five alternate splice forms of the cytoplasmic domain vary by 12 to 48 aa. Within the ECD, human beta 1 shares 92-96% aa sequence identity with rat, bovine, mouse, and feline beta 1. It shares 35-45% aa sequence identity with other beta chains. alpha 5/ beta 1 binds fibronectin in both RGD-dependent and -independent manners (4, 5). alpha 5/ beta 1 is up‑regulated on tumor vasculature and promotes angiogenesis (6, 7). This is accomplished in part by a constitutive association in cis of alpha 5/ beta 1 with VEGFR3 and Tie2, a requirement for optimal activation of those receptors (8, 9). alpha 5/ beta 1 interacts with a variety of other proteins, including HER2, uPAR, Galectin‑1, CTGF, and thrombin-cleaved Osteopontin (5, 10-13). alpha 5/ beta 1 also functions on some hematopoietic and neuronal stem cells (14, 15).

1. Mao, Y. and J.E. Schwarzbauer (2005) Matrix Biol. 24:389.
2. Gu, J. and N. Taniguchi (2004) Glycoconj. J. 21:9.
3. Argraves, W.S. et al. (1987) J. Cell Biol. 105:1183.
4. Takagi, J. et al. (2003) EMBO J. 22:4607.
5. Wei, Y. et al. (2005) J. Cell Biol. 168:501.
6. Parsons-Wingerter, P. et al. (2005) Am. J. Pathol. 167:193.
7. Kim, S. et al. (2000) Am. J. Pathol. 156:1345.
8. Zhang, X. et al. (2004) J. Cell. Physiol. 202:205.
9. Cascone, I. et al. (2005) J. Cell Biol. 170:993.
10. Kuwada, S.K. et al. (2005) J. Biol. Chem. 280:19027.
11. Fischer, C. et al. (2005) J. Biol. Chem. 280:37266.
12. Gao, R. and D.R. Brigstock (2005) Gut 129:1019
13. Yokosaki, Y. et al. (2005) Matrix Biol. 24:418.
14. Carstanjen, D. et al. (2005) Transfusion 45:1192.
15. Gibson, R.M. et al. (2005) Mol. Cell. Neurosci. 28:588.

Publications for Integrin alpha 5 beta 1 (3230-A5)(20)

Publications using 3230-A5

• K Ning, W Zou, P Xu, F Cheng, EY Zhang, A Zhang-Chen, S Kleiboeker, J Qiu Identification of AXL as a co-receptor for human parvovirus B19 infection of human erythroid progenitors Science Advances, 2023-01-11;9(2):eade0869. 2023-01-11 [PMID: 36630517] (Neutralization, Human)
• N Gerwin, C Scotti, C Halleux, M Fornaro, J Elliott, Y Zhang, K Johnson, J Shi, S Walter, Y Li, C Jacobi, N Laplanche, M Belaud, J Paul, G Glowacki, T Peters, KA Wharton, I Vostiar, F Polus, I Kramer, S Guth, A Seroutou, S Choudhury, D Laurent, J Gimbel, J Goldhahn, M Schieker, S Brachat, R Roubenoff, M Kneissel Angiopoietin-like 3-derivative LNA043 for cartilage regeneration in osteoarthritis: a randomized phase 1 trial Nature Medicine, 2022;0(0):. 2022 [PMID: 36456835] (Immunoprecipitation, Human)
• N Gerwin, C Scotti, C Halleux, M Fornaro, J Elliott, Y Zhang, K Johnson, J Shi, S Walter, Y Li, C Jacobi, N Laplanche, M Belaud, J Paul, G Glowacki, T Peters, KA Wharton, I Vostiar, F Polus, I Kramer, S Guth, A Seroutou, S Choudhury, D Laurent, J Gimbel, J Goldhahn, M Schieker, S Brachat, R Roubenoff, M Kneissel Angiopoietin-like 3-derivative LNA043 for cartilage regeneration in osteoarthritis: a randomized phase 1 trial Nature Medicine, 2022-12-01;0(0):. 2022-12-01 [PMID: 36456835] (Immunoprecipitation, Human)
• C Cegarra, B Cameron, C Chaves, T Dabdoubi, TM Do, B Genêt, V Roudières, Y Shi, P Tchepikoff, D Lesuisse An innovative strategy to identify new targets for delivering antibodies to the brain has led to the exploration of the integrin family PLoS ONE, 2022-09-15;17(9):e0274667. 2022-09-15 [PMID: 36108060] (ELISA Capture, Human)
• EG Norris, XS Pan, DC Hocking Receptor binding domain of SARS-CoV-2 is a functional alphav-integrin agonist bioRxiv : the preprint server for biology, 2022-04-11;0(0):. 2022-04-11 [PMID: 35441172] (Surface Plasmon Resonance (SPR, N/A)
• F Irie, Y Tobisawa, A Murao, H Yamamoto, C Ohyama, Y Yamaguchi The Cell Surface Hyaluronidase TMEM2 Regulates Cell Adhesion and Migration via Degradation of Hyaluronan at Focal Adhesion Sites The Journal of Biological Chemistry, 2021-02-26;0(0):100481. 2021-02-26 [PMID: 33647313] (Bioassay, Human)
• S Rattila, CEE Dunk, M Im, O Grichenko, Y Zhou, M Yanez-Mo, SM Blois, KM Yamada, O Erez, N Gomez-Lope, SJ Lye, B Hinz, R Romero, M Cohen, G Dveksler Interaction of Pregnancy-Specific Glycoprotein 1 With Integrin alpha5beta1 Is a Modulator of Extravillous Trophoblast Functions Cells, 2019-10-31;8(11):. 2019-10-31 [PMID: 31683744] (ELISA Standard, Human)
• Kuniyuki Nakamura, Tomoko Ikeuchi, Kazuki Nara, Craig S. Rhodes, Peipei Zhang, Yuta Chiba et al. Perlecan regulates pericyte dynamics in the maintenance and repair of the blood–brain barrier Journal of Cell Biology 2019-10-07 [PMID: 31541017] (Surface Plasmon Resonance (SPR), N/A)
• PR Kuninty, R Bansal, SWL De Geus, DF Mardhian, J Schnittert, J van Baarle, G Storm, MF Bijlsma, HW van Laarho, JM Metselaar, PJK Kuppen, AL Vahrmeijer, A Östman, CFM Sier, J Prakash ITGA5 inhibition in pancreatic stellate cells attenuates desmoplasia and potentiates efficacy of chemotherapy in pancreatic cancer Sci Adv, 2019-09-04;5(9):eaax2770. 2019-09-04 [PMID: 31517053] (Bioassay, Human)
• EC Skoog, VA Morikis, ME Martin, GA Foster, LP Cai, LM Hansen, B Li, JA Gaddy, SI Simon, JV Solnick CagY-Dependent Regulation of Type IV Secretion in Helicobacter pylori Is Associated with Alterations in Integrin Binding MBio, 2018-05-15;9(3):. 2018-05-15 [PMID: 29764950] (Bioassay)
• MA Barbhuiya, AC Mirando, BW Simons, G Lemtiri-Ch, JJ Green, AS Popel, NB Pandey, PT Tran Therapeutic potential of an anti-angiogenic multimodal biomimetic peptide in hepatocellular carcinoma Oncotarget, 2017-09-21;8(60):101520-101534. 2017-09-21 [PMID: 29254183] (Bioassay, Human)
• Systematic site-directed mutagenesis of the Helicobacter pylori CagL protein of the Cag type IV secretion system identifies novel functional domains Sci Rep, 2016-12-06;6(0):38101. 2016-12-06 [PMID: 27922023] (ELISA Developmet, Human)
• Brown A, Dysart M, Clarke K, Stabenfeldt S, Barker T Integrin alpha3beta1 Binding to Fibronectin Is Dependent on the Ninth Type III Repeat. J Biol Chem, 2015-08-28;290(42):25534-47. 2015-08-28 [PMID: 26318455] (Bioassay, Human)
• Qiao L, Gao H, Zhang T, Jing L, Xiao C, Xiao Y, Luo N, Zhu H, Meng W, Xu H, Mo X Snail modulates the assembly of fibronectin via alpha5 integrin for myocardial migration in zebrafish embryos. Sci Rep, 2014-03-26;4(0):4470. 2014-03-26 [PMID: 24667151] (In Vivo, Zebrafish)
• Stampolidis P, Ullrich A, Iacobelli S LGALS3BP, lectin galactoside-binding soluble 3 binding protein, promotes oncogenic cellular events impeded by antibody intervention. Oncogene, 2013-12-23;34(1):39-52. 2013-12-23 [PMID: 24362527] (Bioassay, Human)
• Kwon I, Hong S, Kim Y, Nam H, Kang S, Yang S, Heo J Thrombolytic effects of the snake venom disintegrin saxatilin determined by novel assessment methods: a FeCl3-induced thrombosis model in mice. PLoS ONE, 2013-11-18;8(11):e81165. 2013-11-18 [PMID: 24260554] (Binding Assay)
• Haidari M, Zhang W, Caivano A, Chen Z, Ganjehei L, Mortazavi A, Stroud C, Woodside D, Willerson J, Dixon R Integrin alpha2beta1 mediates tyrosine phosphorylation of vascular endothelial cadherin induced by invasive breast cancer cells. J Biol Chem, 2012-07-25;287(39):32981-92. 2012-07-25 [PMID: 22833667] (Bioassay, Human)
• Shroff K, Kokkoli E PEGylated liposomal doxorubicin targeted to alpha5beta1-expressing MDA-MB-231 breast cancer cells. Langmuir, 2012-02-29;28(10):4729-36. 2012-02-29 [PMID: 22268611] (Bioassay, Human)
• Martchenko M, Jeong SY, Cohen SN Heterodimeric integrin complexes containing beta1-integrin promote internalization and lethality of anthrax toxin. Proc. Natl. Acad. Sci. U.S.A., 2010-08-16;107(35):15583-8. 2010-08-16 [PMID: 20713715] (Bioassay, Mouse)
• Jullienne B, Vigant F, Muth E, Chaligne R, Bouquet C, Giraudier S, Perricaudet M, Benihoud K Efficient delivery of angiostatin K1-5 into tumors following insertion of an NGR peptide into adenovirus capsid. Gene Ther., 2009-12-01;16(12):1405-15. 2009-12-01 [PMID: 19641530] (Bioassay, Human)

Bioinformatics

• Gene Symbol: ITGA5
• Uniprot:
  • P08648 (Integrin alpha 5) & P05556 (Integrin beta 1)()