Recombinant Human Integrin alpha 5 beta 1 Protein, CF

• Reactivity: Hu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Human Integrin alpha 5 beta 1 Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. Recombinant Human Integrin alpha 5 beta 1 binds Bovine Fibronectin (Catalog # 1030-FN) coated at 2 µg/mL with an apparent K_d <0.5 nM in the presence of 1 mM MnCl₂.
• Source: Chinese Hamster Ovary cell line, CHO-derived human Integrin alpha 5 beta 1 protein
  

  Human Integrin alpha 5 (Phe42-Tyr995) Accession # P08648	acidic tail
  Human Integrin beta 1 (Gln21-Asp728) Accession # P05556	basic tail
  N-terminus	C-terminus

• Accession #: P08648 (Integrin alpha 5) & P05556 (Integrin beta 1)
• N-terminal Sequence: Phe42 (Integrin alpha 5) & No results obtained: Gln21 predicted (Integrin beta 1)
• Protein/Peptide Type: Recombinant Proteins
• Gene: ITGA5
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Theoretical MW: 108 kDa ( alpha 5 subunit), 82.3 kDa ( beta 1 subunit).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 105-150 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Integrin alpha 5 beta 1 Protein, CF

• Integrin alpha 5 beta 1
• VLA-5

Background

Integrin alpha 5/ beta 1, also known as VLA-5, is a widely expressed non-covalent heterodimer of a 160 kDa alpha 5 and a 130 kDa beta 1 Integrin subunit. alpha 5/ beta 1 functions in cell adhesion, migration, activation, and matrix fibrillogenesis (1, 2). The human Integrin alpha 5/CD49e cDNA encodes a 1049 amino acid (aa) precursor that includes a 41 aa signal sequence, a 954 aa extracellular domain (ECD), a 26 aa transmembrane segment, and a 28 aa cytoplasmic domain. The ECD contains seven FG-GAP repeats and nine internal disulfide bonds (3). Within the ECD, human alpha 5 shares 90% and 46% aa sequence identity with mouse alpha 5 and human alpha 8, respectively, and less than 30% aa sequence identitity with other human alpha chains. The human Integrin beta 1/CD29 cDNA encodes a 798 aa precursor that includes a 20 aa signal sequence, a 708 aa ECD, a 23 aa transmembrane segment, and a 47 aa cytoplasmic domain. The ECD contains one vWF-A domain, four Cys-rich repeats, and 29 internal disulfide bonds (3). Five alternate splice forms of the cytoplasmic domain vary by 12 to 48 aa. Within the ECD, human beta 1 shares 92-96% aa sequence identity with rat, bovine, mouse, and feline beta 1. It shares 35-45% aa sequence identity with other beta chains. alpha 5/ beta 1 binds fibronectin in both RGD-dependent and -independent manners (4, 5). alpha 5/ beta 1 is up‑regulated on tumor vasculature and promotes angiogenesis (6, 7). This is accomplished in part by a constitutive association in cis of alpha 5/ beta 1 with VEGFR3 and Tie2, a requirement for optimal activation of those receptors (8, 9). alpha 5/ beta 1 interacts with a variety of other proteins, including HER2, uPAR, Galectin‑1, CTGF, and thrombin-cleaved Osteopontin (5, 10-13). alpha 5/ beta 1 also functions on some hematopoietic and neuronal stem cells (14, 15).

1. Mao, Y. and J.E. Schwarzbauer (2005) Matrix Biol. 24:389.
2. Gu, J. and N. Taniguchi (2004) Glycoconj. J. 21:9.
3. Argraves, W.S. et al. (1987) J. Cell Biol. 105:1183.
4. Takagi, J. et al. (2003) EMBO J. 22:4607.
5. Wei, Y. et al. (2005) J. Cell Biol. 168:501.
6. Parsons-Wingerter, P. et al. (2005) Am. J. Pathol. 167:193.
7. Kim, S. et al. (2000) Am. J. Pathol. 156:1345.
8. Zhang, X. et al. (2004) J. Cell. Physiol. 202:205.
9. Cascone, I. et al. (2005) J. Cell Biol. 170:993.
10. Kuwada, S.K. et al. (2005) J. Biol. Chem. 280:19027.
11. Fischer, C. et al. (2005) J. Biol. Chem. 280:37266.
12. Gao, R. and D.R. Brigstock (2005) Gut 129:1019
13. Yokosaki, Y. et al. (2005) Matrix Biol. 24:418.
14. Carstanjen, D. et al. (2005) Transfusion 45:1192.
15. Gibson, R.M. et al. (2005) Mol. Cell. Neurosci. 28:588.

Publications for Integrin alpha 5 beta 1 (3230-A5)(10)

Publications using 3230-A5

• EC Skoog, VA Morikis, ME Martin, GA Foster, LP Cai, LM Hansen, B Li, JA Gaddy, SI Simon, JV Solnick CagY-Dependent Regulation of Type IV Secretion in Helicobacter pylori Is Associated with Alterations in Integrin Binding MBio, 2018;9(3):. 2018 [PMID: 29764950] (Bioassay)
• Systematic site-directed mutagenesis of the Helicobacter pylori CagL protein of the Cag type IV secretion system identifies novel functional domains Sci Rep, 2016;6(0):38101. 2016 [PMID: 27922023] (ELISA development, Human)
• Brown A, Dysart M, Clarke K, Stabenfeldt S, Barker T Integrin alpha3beta1 Binding to Fibronectin Is Dependent on the Ninth Type III Repeat. J Biol Chem, 2015;290(42):25534-47. 2015 [PMID: 26318455] (Bioassay, Human)
• Qiao L, Gao H, Zhang T, Jing L, Xiao C, Xiao Y, Luo N, Zhu H, Meng W, Xu H, Mo X Snail modulates the assembly of fibronectin via alpha5 integrin for myocardial migration in zebrafish embryos. Sci Rep, 2014;4(0):4470. 2014 [PMID: 24667151] (In Vivo, Zebrafish)
• Stampolidis P, Ullrich A, Iacobelli S LGALS3BP, lectin galactoside-binding soluble 3 binding protein, promotes oncogenic cellular events impeded by antibody intervention. Oncogene, 2015;34(1):39-52. 2015 [PMID: 24362527] (Bioassay, Human)
• Kwon I, Hong S, Kim Y, Nam H, Kang S, Yang S, Heo J Thrombolytic effects of the snake venom disintegrin saxatilin determined by novel assessment methods: a FeCl3-induced thrombosis model in mice. PLoS ONE, 2013;8(11):e81165. 2013 [PMID: 24260554] (Binding Assay, N/A)
• Haidari M, Zhang W, Caivano A, Chen Z, Ganjehei L, Mortazavi A, Stroud C, Woodside D, Willerson J, Dixon R Integrin alpha2beta1 mediates tyrosine phosphorylation of vascular endothelial cadherin induced by invasive breast cancer cells. J Biol Chem, 2012;287(39):32981-92. 2012 [PMID: 22833667] (Bioassay, Human)
• Shroff K, Kokkoli E PEGylated liposomal doxorubicin targeted to alpha5beta1-expressing MDA-MB-231 breast cancer cells. Langmuir, 2012;28(10):4729-36. 2012 [PMID: 22268611] (Bioassay, Human)
• Martchenko M, Jeong SY, Cohen SN Heterodimeric integrin complexes containing beta1-integrin promote internalization and lethality of anthrax toxin. Proc. Natl. Acad. Sci. U.S.A., 2010;107(35):15583-8. 2010 [PMID: 20713715]
• Jullienne B, Vigant F, Muth E, Chaligne R, Bouquet C, Giraudier S, Perricaudet M, Benihoud K Efficient delivery of angiostatin K1-5 into tumors following insertion of an NGR peptide into adenovirus capsid. Gene Ther., 2009;16(12):1405-15. 2009 [PMID: 19641530] (Bioassay, Human)

Bioinformatics

• Gene Symbol: ITGA5
• Uniprot:
  • P08648 (Integrin alpha 5) & P05556 (Integrin beta 1)()