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Recombinant Human Integrin alpha 5 beta 1 Protein, CF Summary
Details of Functionality
Measured by its binding ability in a functional ELISA. Recombinant Human Integrin alpha 5 beta 1 binds Bovine Fibronectin (Catalog # 1030-FN) coated at 2 µg/mL with an apparent Kd <0.5 nM in the presence of 1 mM MnCl2.
Source
Chinese Hamster Ovary cell line, CHO-derived human Integrin alpha 5 beta 1 protein
Human Integrin alpha 5 (Phe42-Tyr995) Accession # P08648
acidic tail
Human Integrin beta 1 (Gln21-Asp728) Accession # P05556
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.
Applications/Dilutions
Dilutions
Binding Activity
Theoretical MW
108 kDa ( alpha 5 subunit), 82.3 kDa ( beta 1 subunit). Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
105-150 kDa, reducing conditions
Publications
Read Publications using 3230-A5 in the following applications:
Integrin alpha 5/ beta 1, also known as VLA-5, is a widely expressed non-covalent heterodimer of a 160 kDa alpha 5 and a 130 kDa beta 1 Integrin subunit. alpha 5/ beta 1 functions in cell adhesion, migration, activation, and matrix fibrillogenesis (1, 2). The human Integrin alpha 5/CD49e cDNA encodes a 1049 amino acid (aa) precursor that includes a 41 aa signal sequence, a 954 aa extracellular domain (ECD), a 26 aa transmembrane segment, and a 28 aa cytoplasmic domain. The ECD contains seven FG-GAP repeats and nine internal disulfide bonds (3). Within the ECD, human alpha 5 shares 90% and 46% aa sequence identity with mouse alpha 5 and human alpha 8, respectively, and less than 30% aa sequence identitity with other human alpha chains. The human Integrin beta 1/CD29 cDNA encodes a 798 aa precursor that includes a 20 aa signal sequence, a 708 aa ECD, a 23 aa transmembrane segment, and a 47 aa cytoplasmic domain. The ECD contains one vWF-A domain, four Cys-rich repeats, and 29 internal disulfide bonds (3). Five alternate splice forms of the cytoplasmic domain vary by 12 to 48 aa. Within the ECD, human beta 1 shares 92-96% aa sequence identity with rat, bovine, mouse, and feline beta 1. It shares 35-45% aa sequence identity with other beta chains. alpha 5/ beta 1 binds fibronectin in both RGD-dependent and -independent manners (4, 5). alpha 5/ beta 1 is up‑regulated on tumor vasculature and promotes angiogenesis (6, 7). This is accomplished in part by a constitutive association in cis of alpha 5/ beta 1 with VEGFR3 and Tie2, a requirement for optimal activation of those receptors (8, 9). alpha 5/ beta 1 interacts with a variety of other proteins, including HER2, uPAR, Galectin‑1, CTGF, and thrombin-cleaved Osteopontin (5, 10-13). alpha 5/ beta 1 also functions on some hematopoietic and neuronal stem cells (14, 15).
Mao, Y. and J.E. Schwarzbauer (2005) Matrix Biol. 24:389.
Gu, J. and N. Taniguchi (2004) Glycoconj. J. 21:9.
Argraves, W.S. et al. (1987) J. Cell Biol. 105:1183.
Takagi, J. et al. (2003) EMBO J. 22:4607.
Wei, Y. et al. (2005) J. Cell Biol. 168:501.
Parsons-Wingerter, P. et al. (2005) Am. J. Pathol. 167:193.
Kim, S. et al. (2000) Am. J. Pathol. 156:1345.
Zhang, X. et al. (2004) J. Cell. Physiol. 202:205.
Cascone, I. et al. (2005) J. Cell Biol. 170:993.
Kuwada, S.K. et al. (2005) J. Biol. Chem. 280:19027.
Fischer, C. et al. (2005) J. Biol. Chem. 280:37266.
Gao, R. and D.R. Brigstock (2005) Gut 129:1019
Yokosaki, Y. et al. (2005) Matrix Biol. 24:418.
Carstanjen, D. et al. (2005) Transfusion 45:1192.
Gibson, R.M. et al. (2005) Mol. Cell. Neurosci. 28:588.
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