Recombinant Human Integrin alpha 5 beta 1 Protein, CF

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    • Catalog Number
      3230-A5
    • Availability
      Product Discontinued

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Recombinant Human Integrin alpha 5 beta 1 Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. Recombinant Human Integrin alpha 5 beta 1 binds Bovine Fibronectin (Catalog # 1030-FN) coated at 2 µg/mL with an apparent Kd <0.5 nM in the presence of 1 mM MnCl2.
Source
Chinese Hamster Ovary cell line, CHO-derived human Integrin alpha 5 beta 1 protein
Human Integrin alpha 5
(Phe42-Tyr995)
Accession # P08648
acidic tail
Human Integrin beta 1
(Gln21-Asp728)
Accession # P05556
basic tail
N-terminus C-terminus
Accession #
N-terminal Sequence
Phe42 (Integrin alpha 5) & No results obtained: Gln21 predicted (Integrin beta 1)
Protein/Peptide Type
Recombinant Proteins
Gene
ITGA5
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Binding Activity
Theoretical MW
108 kDa ( alpha 5 subunit), 82.3 kDa ( beta 1 subunit).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
105-150 kDa, reducing conditions
Publications
Read Publications using
3230-A5 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Integrin alpha 5 beta 1 Protein, CF

  • CD49 antigen-like family member E
  • CD49e
  • Fibronectin receptor subunit alpha
  • fibronectin receptor, alpha polypeptide
  • fibronectin receptor, alpha subunit
  • FNRA
  • Integrin alpha 5 beta 1
  • integrin alpha-5
  • Integrin alpha-F
  • integrin, alpha 5 (fibronectin receptor, alpha polypeptide)
  • ITGA5/SLC9B1 fusion
  • very late activation protein 5, alpha subunit
  • VLA-5
  • VLA5A

Background

Integrin alpha 5/ beta 1, also known as VLA-5, is a widely expressed non-covalent heterodimer of a 160 kDa alpha 5 and a 130 kDa beta 1 Integrin subunit. alpha 5/ beta 1 functions in cell adhesion, migration, activation, and matrix fibrillogenesis (1, 2). The human Integrin alpha 5/CD49e cDNA encodes a 1049 amino acid (aa) precursor that includes a 41 aa signal sequence, a 954 aa extracellular domain (ECD), a 26 aa transmembrane segment, and a 28 aa cytoplasmic domain. The ECD contains seven FG-GAP repeats and nine internal disulfide bonds (3). Within the ECD, human alpha 5 shares 90% and 46% aa sequence identity with mouse alpha 5 and human alpha 8, respectively, and less than 30% aa sequence identitity with other human alpha chains. The human Integrin beta 1/CD29 cDNA encodes a 798 aa precursor that includes a 20 aa signal sequence, a 708 aa ECD, a 23 aa transmembrane segment, and a 47 aa cytoplasmic domain. The ECD contains one vWF-A domain, four Cys-rich repeats, and 29 internal disulfide bonds (3). Five alternate splice forms of the cytoplasmic domain vary by 12 to 48 aa. Within the ECD, human beta 1 shares 92-96% aa sequence identity with rat, bovine, mouse, and feline beta 1. It shares 35-45% aa sequence identity with other beta chains. alpha 5/ beta 1 binds fibronectin in both RGD-dependent and -independent manners (4, 5). alpha 5/ beta 1 is up‑regulated on tumor vasculature and promotes angiogenesis (6, 7). This is accomplished in part by a constitutive association in cis of alpha 5/ beta 1 with VEGFR3 and Tie2, a requirement for optimal activation of those receptors (8, 9). alpha 5/ beta 1 interacts with a variety of other proteins, including HER2, uPAR, Galectin‑1, CTGF, and thrombin-cleaved Osteopontin (5, 10-13). alpha 5/ beta 1 also functions on some hematopoietic and neuronal stem cells (14, 15).

  1. Mao, Y. and J.E. Schwarzbauer (2005) Matrix Biol. 24:389.
  2. Gu, J. and N. Taniguchi (2004) Glycoconj. J. 21:9.
  3. Argraves, W.S. et al. (1987) J. Cell Biol. 105:1183.
  4. Takagi, J. et al. (2003) EMBO J. 22:4607.
  5. Wei, Y. et al. (2005) J. Cell Biol. 168:501.
  6. Parsons-Wingerter, P. et al. (2005) Am. J. Pathol. 167:193.
  7. Kim, S. et al. (2000) Am. J. Pathol. 156:1345.
  8. Zhang, X. et al. (2004) J. Cell. Physiol. 202:205.
  9. Cascone, I. et al. (2005) J. Cell Biol. 170:993.
  10. Kuwada, S.K. et al. (2005) J. Biol. Chem. 280:19027.
  11. Fischer, C. et al. (2005) J. Biol. Chem. 280:37266.
  12. Gao, R. and D.R. Brigstock (2005) Gut 129:1019
  13. Yokosaki, Y. et al. (2005) Matrix Biol. 24:418.
  14. Carstanjen, D. et al. (2005) Transfusion 45:1192.
  15. Gibson, R.M. et al. (2005) Mol. Cell. Neurosci. 28:588.

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Publications for Integrin alpha 5 beta 1 (3230-A5)(20)

We have publications tested in 4 confirmed species: Human, Mouse, N/A, Zebrafish.

We have publications tested in 10 applications: Binding Assay, Bioassay, ELISA Capture, ELISA Developmet, ELISA Standard, Immunoprecipitation, In Vivo, Neutralization, Surface Plasmon Resonance (SPR, Surface Plasmon Resonance (SPR).


Filter By Application
Binding Assay
(1)
Bioassay
(10)
ELISA Capture
(1)
ELISA Developmet
(1)
ELISA Standard
(1)
Immunoprecipitation
(2)
In Vivo
(1)
Neutralization
(1)
Surface Plasmon Resonance (SPR
(1)
Surface Plasmon Resonance (SPR)
(1)
All Applications
Filter By Species
Human
(14)
Mouse
(1)
N/A
(2)
Zebrafish
(1)
All Species
Showing Publications 1 - 10 of 20. Show All 20 Publications.
Publications using 3230-A5 Applications Species
K Ning, W Zou, P Xu, F Cheng, EY Zhang, A Zhang-Chen, S Kleiboeker, J Qiu Identification of AXL as a co-receptor for human parvovirus B19 infection of human erythroid progenitors Science Advances, 2023-01-11;9(2):eade0869. 2023-01-11 [PMID: 36630517] (Neutralization, Human) Neutralization Human
N Gerwin, C Scotti, C Halleux, M Fornaro, J Elliott, Y Zhang, K Johnson, J Shi, S Walter, Y Li, C Jacobi, N Laplanche, M Belaud, J Paul, G Glowacki, T Peters, KA Wharton, I Vostiar, F Polus, I Kramer, S Guth, A Seroutou, S Choudhury, D Laurent, J Gimbel, J Goldhahn, M Schieker, S Brachat, R Roubenoff, M Kneissel Angiopoietin-like 3-derivative LNA043 for cartilage regeneration in osteoarthritis: a randomized phase 1 trial Nature Medicine, 2022;0(0):. 2022 [PMID: 36456835] (Immunoprecipitation, Human) Immunoprecipitation Human
N Gerwin, C Scotti, C Halleux, M Fornaro, J Elliott, Y Zhang, K Johnson, J Shi, S Walter, Y Li, C Jacobi, N Laplanche, M Belaud, J Paul, G Glowacki, T Peters, KA Wharton, I Vostiar, F Polus, I Kramer, S Guth, A Seroutou, S Choudhury, D Laurent, J Gimbel, J Goldhahn, M Schieker, S Brachat, R Roubenoff, M Kneissel Angiopoietin-like 3-derivative LNA043 for cartilage regeneration in osteoarthritis: a randomized phase 1 trial Nature Medicine, 2022-12-01;0(0):. 2022-12-01 [PMID: 36456835] (Immunoprecipitation, Human) Immunoprecipitation Human
C Cegarra, B Cameron, C Chaves, T Dabdoubi, TM Do, B Genêt, V Roudières, Y Shi, P Tchepikoff, D Lesuisse An innovative strategy to identify new targets for delivering antibodies to the brain has led to the exploration of the integrin family PLoS ONE, 2022-09-15;17(9):e0274667. 2022-09-15 [PMID: 36108060] (ELISA Capture, Human) ELISA Capture Human
EG Norris, XS Pan, DC Hocking Receptor binding domain of SARS-CoV-2 is a functional alphav-integrin agonist bioRxiv : the preprint server for biology, 2022-04-11;0(0):. 2022-04-11 [PMID: 35441172] (Surface Plasmon Resonance (SPR, N/A) Surface Plasmon Resonance (SPR N/A
F Irie, Y Tobisawa, A Murao, H Yamamoto, C Ohyama, Y Yamaguchi The Cell Surface Hyaluronidase TMEM2 Regulates Cell Adhesion and Migration via Degradation of Hyaluronan at Focal Adhesion Sites The Journal of Biological Chemistry, 2021-02-26;0(0):100481. 2021-02-26 [PMID: 33647313] (Bioassay, Human) Bioassay Human
S Rattila, CEE Dunk, M Im, O Grichenko, Y Zhou, M Yanez-Mo, SM Blois, KM Yamada, O Erez, N Gomez-Lope, SJ Lye, B Hinz, R Romero, M Cohen, G Dveksler Interaction of Pregnancy-Specific Glycoprotein 1 With Integrin alpha5beta1 Is a Modulator of Extravillous Trophoblast Functions Cells, 2019-10-31;8(11):. 2019-10-31 [PMID: 31683744] (ELISA Standard, Human) ELISA Standard Human
Kuniyuki Nakamura, Tomoko Ikeuchi, Kazuki Nara, Craig S. Rhodes, Peipei Zhang, Yuta Chiba et al. Perlecan regulates pericyte dynamics in the maintenance and repair of the blood–brain barrier Journal of Cell Biology 2019-10-07 [PMID: 31541017] (Surface Plasmon Resonance (SPR), N/A) Surface Plasmon Resonance (SPR) N/A
PR Kuninty, R Bansal, SWL De Geus, DF Mardhian, J Schnittert, J van Baarle, G Storm, MF Bijlsma, HW van Laarho, JM Metselaar, PJK Kuppen, AL Vahrmeijer, A Östman, CFM Sier, J Prakash ITGA5 inhibition in pancreatic stellate cells attenuates desmoplasia and potentiates efficacy of chemotherapy in pancreatic cancer Sci Adv, 2019-09-04;5(9):eaax2770. 2019-09-04 [PMID: 31517053] (Bioassay, Human) Bioassay Human
EC Skoog, VA Morikis, ME Martin, GA Foster, LP Cai, LM Hansen, B Li, JA Gaddy, SI Simon, JV Solnick CagY-Dependent Regulation of Type IV Secretion in Helicobacter pylori Is Associated with Alterations in Integrin Binding MBio, 2018-05-15;9(3):. 2018-05-15 [PMID: 29764950] (Bioassay) Bioassay
Show All 20 Publications.

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