Recombinant Human IL-24 Protein, CF

Images

 
There are currently no images for IL-24 (1965-IL/CF).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Recombinant Human IL-24 Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using BaF3 mouse pro‑B cells transfected with human IL-20 R alpha and human IL-20 R beta. The ED50 for this effect is 15-150 pg/mL.
Source
Mouse myeloma cell line, NS0-derived human IL-24 protein
Met28-Leu206, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Gln52 predicted, no results obtained, sequencing might be blocked.
Protein/Peptide Type
Recombinant Proteins
Gene
IL24
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
19.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
26-35 kDa, reducing conditions
Publications
Read Publications using
1965-IL/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-24 Protein, CF

  • C49A
  • FISP
  • IL10B
  • IL24
  • IL-24
  • interleukin 24
  • MDA-7
  • MDA7MOB5
  • melanocyte-associated Mda-7
  • melanoma differentiation association protein 7
  • Melanoma differentiation-associated gene 7 protein
  • MOB-5
  • ST16
  • ST16interleukin-24
  • suppression of tumorigenicity 16 (melanoma differentiation)
  • Suppression of tumorigenicity 16 protein

Background

Interleukin 24 (IL-24), also known as mda-7 (melanoma differentiation associated gene-7), is a member of the IL-10 family of helical cytokines. The IL-24 gene encodes a precursor protein of 207 amino acids (aa) that contains a 48 aa signal sequence and an 18 kDa, 158 aa mature segment. There are three potential N-linked glycosylation sites, at least one of which is used. When secreted, IL-24 is a 35 - 40 kDa phosphorylated glycoprotein that apparently can exist as either a monomer or dimer. It is suggested that glycosylation is essential for activity. Mature human IL-24 shares 69% aa sequence identity with mouse and rat IL-24. Human IL-24 is also active in rodent systems. Cells known to express IL-24 include B cells, CD4+ T cells, NK cells, lymph node dendritic cells, monocytes, melanocytes, and melanoma cells. Functionally, IL‑24 has diverse activities. At low concentrations on monocytes, it induces type I proinflammatory cytokines such as IFN‑ gamma, IL‑1 beta, IL‑12 and TNF-alpha. At high concentrations, it is a strong inducer of apoptosis in tumor cells, but not normal cells. IL‑24 also has anti-angiogenic properties. It directly binds IL-24 receptors on endothelial cells, activating STAT3 and blocking their differentiation. IL-24 binds and signals through two heterodimeric receptor complexes. One complex is the combination of IL‑20 R alpha and IL-20 R beta, which is shared with IL-19 and IL‑20. The second complex is a combination of IL‑22 R and IL-20 R beta, which is shared with IL-20.

  1. Jiang, H. et al. (1995) Oncogene 11:2477.
  2. Jiang, H. et al. (1996) Proc. Natl. Acad. Sci. USA 93:9160.
  3. Wang, M. et al. (2002) J. Biol Chem. 227:7341.
  4. Chada, S. et al. (2004) Int. Immunopharmacol. 4:649.
  5. Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.
  6. Chen, J. et al. (2003) Molec. Ther. 8:220.

Customers Who Viewed This Item Also Viewed...

AF1709
Species: Mu
Applications: WB, Block, CyTOF-ready, ELISA(Cap), ELISA(Det), ICFlow, ELISA(Sta)
1102-IL/CF
Species: Hu
NBP2-20619
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-71770
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NBP2-01863
Species: Hu
Applications: WB, IHC, IHC-P
NB200-585
Species: Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready, Flow-IC
NBP1-86919
Species: Hu
Applications: WB, IHC, IHC-P
NB100-56098
Species: Hu, Mu, Rt, Ca
Applications: WB, IHC, IHC-P, IP
AF800
Species: Hu, Mu
Applications: WB, Simple Western, IP
NBP1-28566
Species: Hu, Mu, Rt, Bv, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IP
NB200-103
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
NB100-56409
Species: Hu, Mu
Applications: WB, Flow
NB100-56708
Species: Hu, Mu, Rt, Po, Ch, ChHa, Ma
Applications: WB, Simple Western, EM, Flow, HEStain, IB, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready, KO
NBP2-67471
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P

Publications for IL-24 (1965-IL/CF)(14)

We have publications tested in 2 confirmed species: Human, N/A.

We have publications tested in 2 applications: Bioassay, ELISA (Standard).


Filter By Application
Bioassay
(13)
ELISA (Standard)
(1)
All Applications
Filter By Species
Human
(13)
N/A
(1)
All Species
Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using 1965-IL/CF Applications Species
NP Kumar, K Moideen, VV Banurekha, D Nair, S Babu Modulation of Th1/Tc1 and Th17/Tc17 responses in pulmonary tuberculosis by IL-20 subfamily of cytokines Cytokine, 2018;108(0):190-196. 2018 [PMID: 29684756] (Bioassay, Human) Bioassay Human
S Kawada, Y Nagasawa, M Kawabe, H Ohyama, A Kida, N Kato-Kogoe, M Nanami, Y Hasuike, T Kuragano, H Kishimoto, K Nakasho, T Nakanishi Iron-induced calcification in human aortic vascular smooth muscle cells through interleukin-24 (IL-24), with/without TNF-alpha Sci Rep, 2018;8(1):658. 2018 [PMID: 29330517] (Bioassay, Human) Bioassay Human
BX Ross, N Gao, X Cui, TJ Standiford, J Xu, FX Yu IL-24 Promotes Pseudomonas aeruginosa Keratitis in C57BL/6 Mouse Corneas J. Immunol, 2017;0(0):. 2017 [PMID: 28330899] (Bioassay, Human) Bioassay Human
D Pap, E Sziksz, Z Kiss, R Rokonay, A Veres-Szék, R Lippai, IM Takács, É Kis, A Fekete, G Reusz, A Vannay, AJ Szabó Microarray Analysis Reveals Increased Expression of Matrix Metalloproteases and Cytokines of Interleukin-20 Subfamily in the Kidneys of Neonate Rats Underwent Unilateral Ureteral Obstruction: A Potential Role of IL-24 in the Regulation of Inflammation and Tissue Remodeling Kidney Blood Press. Res, 2017;42(1):16-32. 2017 [PMID: 28253513] (Bioassay, Human) Bioassay Human
Robert Sabat MMP8 Is Increased in Lesions and Blood of Acne Inversa Patients: A Potential Link to Skin Destruction and Metabolic Alterations Mediators Inflamm., 2016;2016(0):4097574. 2016 [PMID: 27843200] (Bioassay, Human) Bioassay Human
Kumari S, Bonnet M, Ulvmar M, Wolk K, Karagianni N, Witte E, Uthoff-Hachenberg C, Renauld J, Kollias G, Toftgard R, Sabat R, Pasparakis M, Haase I Tumor necrosis factor receptor signaling in keratinocytes triggers interleukin-24-dependent psoriasis-like skin inflammation in mice. Immunity, 2013;39(5):899-911. 2013 [PMID: 24211183] (Bioassay, Human) Bioassay Human
Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 2012;487(7408):505-9. 2012 [PMID: 22763448] (Bioassay, Human) Bioassay Human
Heinemann A, He Y, Zimina E, Boerries M, Busch H, Chmel N, Kurz T, Bruckner-Tuderman L, Has C Induction of phenotype modifying cytokines by FERMT1 mutations. Hum. Mutat., 2011;32(4):397-406. 2011 [PMID: 21309038] (Bioassay, Human) Bioassay Human
Maarof G, Bouchet-Delbos L, Gary-Gouy H, Durand-Gasselin I, Krzysiek R, Dalloul A Interleukin-24 inhibits the plasma cell differentiation program in human germinal center B cells. Blood, 2010;115(9):1718-26. 2010 [PMID: 19965640] (Bioassay, Human) Bioassay Human
Tohyama M, Hanakawa Y, Shirakata Y, Dai X, Yang L, Hirakawa S, Tokumaru S, Okazaki H, Sayama K, Hashimoto K IL-17 and IL-22 mediate IL-20 subfamily cytokine production in cultured keratinocytes via increased IL-22 receptor expression. Eur. J. Immunol., 2009;39(10):2779-88. 2009 [PMID: 19731362] (Bioassay, Human) Bioassay Human
Show All 14 Publications.

Reviews for IL-24 (1965-IL/CF) (0)

There are no reviews for IL-24 (1965-IL/CF). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for IL-24 (1965-IL/CF) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human IL-24 Protein, CF and receive a gift card or discount.

Bioinformatics

Gene Symbol IL24
Uniprot