Recombinant Human IL-24 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human IL-24 Protein, CF Summary

• Details of Functionality: Measured in a cell proliferation assay using BaF3 mouse pro-B cells transfected with human IL-20 R alpha and human IL-20 R beta . The ED 50 for this effect is 15-150 pg/mL.
• Source: Mouse myeloma cell line, NS0-derived human IL-24 protein Met28-Leu206, with a C-terminal 10-His tag
• Accession #: Q13007
• N-terminal Sequence: Gln52 predicted, no results obtained, sequencing might be blocked.
• Protein/Peptide Type: Recombinant Proteins
• Gene: IL24
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 19.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 26-35 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 3 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-24 Protein, CF

• C49A
• FISP
• IL10B
• IL24
• IL-24
• interleukin 24
• MDA-7
• MDA7MOB5
• melanocyte-associated Mda-7
• melanoma differentiation association protein 7
• Melanoma differentiation-associated gene 7 protein
• MOB-5
• ST16
• ST16interleukin-24
• suppression of tumorigenicity 16 (melanoma differentiation)
• Suppression of tumorigenicity 16 protein

Background

Interleukin 24 (IL-24), also known as mda-7 (melanoma differentiation associated gene-7), is a member of the IL-10 family of helical cytokines. The IL-24 gene encodes a precursor protein of 207 amino acids (aa) that contains a 48 aa signal sequence and an 18 kDa, 158 aa mature segment. There are three potential N-linked glycosylation sites, at least one of which is used. When secreted, IL-24 is a 35 - 40 kDa phosphorylated glycoprotein that apparently can exist as either a monomer or dimer. It is suggested that glycosylation is essential for activity. Mature human IL-24 shares 69% aa sequence identity with mouse and rat IL-24. Human IL-24 is also active in rodent systems. Cells known to express IL-24 include B cells, CD4 + T cells, NK cells, lymph node dendritic cells, monocytes, melanocytes, and melanoma cells. Functionally, IL-24 has diverse activities. At low concentrations on monocytes, it induces type I proinflammatory cytokines such as IFN‑ gamma , IL‑1 beta , IL‑12 and TNF-alpha . At high concentrations, it is a strong inducer of apoptosis in tumor cells, but not normal cells. IL‑24 also has anti-angiogenic properties. It directly binds IL-24 receptors on endothelial cells, activating STAT3 and blocking their differentiation. IL-24 binds and signals through two heterodimeric receptor complexes. One complex is the combination of IL-20 R alpha and IL-20 R beta , which is shared with IL-19 and IL-20. The second complex is a combination of IL-22 R and IL-20 R beta , which is shared with IL-20.

1. Jiang, H. et al. (1995) Oncogene 11:2477.
2. Jiang, H. et al. (1996) Proc. Natl. Acad. Sci. USA 93:9160.
3. Wang, M. et al. (2002) J. Biol Chem. 227:7341.
4. Chada, S. et al. (2004) Int. Immunopharmacol. 4:649.
5. Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.
6. Chen, J. et al. (2003) Molec. Ther. 8:220.

Publications for IL-24 (1965-IL/CF)(17)

Publications using 1965-IL/CF

• Y Liu, Y Chen, R Sha, Y Li, T Xu, X Hu, L Xu, Q Xie, B Zhao A new insight into the role of aryl hydrocarbon receptor (AhR) in the migration of glioblastoma by AhR-IL24 axis regulation Environment international, 2021-05-31;154(0):106658. 2021-05-31 [PMID: 34082239] (Bioassay, Human)
• D Pap, A Veres-Szék, B Szebeni, R Rokonay, A Ónody, R Lippai, IM Takács, A Tislér, M Kardos, F Oswald, A Fekete, AJ Szabó, Á Vannay Characterization of IL-19, -20, and -24 in acute and chronic kidney diseases reveals a pro-fibrotic role of IL-24 J Transl Med, 2020-04-19;18(1):172. 2020-04-19 [PMID: 32306980] (Bioassay, Human, Rat)
• D Deli?, K Wolk, R Schmid, O Gabrielyan, D Christou, K Rieber, M Rolser, I Jakob, F Wiech, M Griesser, C Wohnhaas, G Kokolakis, E Witte-Händ, P Baum, R Sabat Integrated microRNA/mRNA expression profiling of the skin of psoriasis patients J. Dermatol. Sci., 2019-11-30;0(0):. 2019-11-30 [PMID: 31843230] (Bioassay, Human)
• NP Kumar, K Moideen, VV Banurekha, D Nair, S Babu Modulation of Th1/Tc1 and Th17/Tc17 responses in pulmonary tuberculosis by IL-20 subfamily of cytokines Cytokine, 2018-04-21;108(0):190-196. 2018-04-21 [PMID: 29684756] (Bioassay, Human)
• S Kawada, Y Nagasawa, M Kawabe, H Ohyama, A Kida, N Kato-Kogoe, M Nanami, Y Hasuike, T Kuragano, H Kishimoto, K Nakasho, T Nakanishi Iron-induced calcification in human aortic vascular smooth muscle cells through interleukin-24 (IL-24), with/without TNF-alpha Sci Rep, 2018-01-12;8(1):658. 2018-01-12 [PMID: 29330517] (Bioassay, Human)
• BX Ross, N Gao, X Cui, TJ Standiford, J Xu, FX Yu IL-24 Promotes Pseudomonas aeruginosa Keratitis in C57BL/6 Mouse Corneas J. Immunol, 2017-03-22;0(0):. 2017-03-22 [PMID: 28330899] (Bioassay, Human)
• D Pap, E Sziksz, Z Kiss, R Rokonay, A Veres-Szék, R Lippai, IM Takács, É Kis, A Fekete, G Reusz, A Vannay, AJ Szabó Microarray Analysis Reveals Increased Expression of Matrix Metalloproteases and Cytokines of Interleukin-20 Subfamily in the Kidneys of Neonate Rats Underwent Unilateral Ureteral Obstruction: A Potential Role of IL-24 in the Regulation of Inflammation and Tissue Remodeling Kidney Blood Press. Res, 2017-02-28;42(1):16-32. 2017-02-28 [PMID: 28253513] (Bioassay, Human)
• Robert Sabat MMP8 Is Increased in Lesions and Blood of Acne Inversa Patients: A Potential Link to Skin Destruction and Metabolic Alterations Mediators Inflamm., 2016-10-23;2016(0):4097574. 2016-10-23 [PMID: 27843200] (Bioassay, Human)
• Kumari S, Bonnet M, Ulvmar M, Wolk K, Karagianni N, Witte E, Uthoff-Hachenberg C, Renauld J, Kollias G, Toftgard R, Sabat R, Pasparakis M, Haase I Tumor necrosis factor receptor signaling in keratinocytes triggers interleukin-24-dependent psoriasis-like skin inflammation in mice. Immunity, 2013-11-07;39(5):899-911. 2013-11-07 [PMID: 24211183] (Bioassay, Human)
• Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 2012-07-26;487(7408):505-9. 2012-07-26 [PMID: 22763448] (Bioassay, Human)
• Heinemann A, He Y, Zimina E, Boerries M, Busch H, Chmel N, Kurz T, Bruckner-Tuderman L, Has C Induction of phenotype modifying cytokines by FERMT1 mutations. Hum. Mutat., 2011-03-01;32(4):397-406. 2011-03-01 [PMID: 21309038] (Bioassay, Human)
• Maarof G, Bouchet-Delbos L, Gary-Gouy H, Durand-Gasselin I, Krzysiek R, Dalloul A Interleukin-24 inhibits the plasma cell differentiation program in human germinal center B cells. Blood, 2009-11-24;115(9):1718-26. 2009-11-24 [PMID: 19965640] (Bioassay, Human)
• Tohyama M, Hanakawa Y, Shirakata Y, Dai X, Yang L, Hirakawa S, Tokumaru S, Okazaki H, Sayama K, Hashimoto K IL-17 and IL-22 mediate IL-20 subfamily cytokine production in cultured keratinocytes via increased IL-22 receptor expression. Eur. J. Immunol., 2009-10-01;39(10):2779-88. 2009-10-01 [PMID: 19731362] (Bioassay, Human)
• Duluc D, Corvaisier M, Blanchard S, Catala L, Descamps P, Gamelin E, Ponsoda S, Delneste Y, Hebbar M, Jeannin P Interferon-gamma reverses the immunosuppressive and protumoral properties and prevents the generation of human tumor-associated macrophages. Int. J. Cancer, 2009-07-15;125(2):367-73. 2009-07-15 [PMID: 19378341] (Bioassay, Human)
• Wu B, Huang C, Kato-Maeda M, Hopewell PC, Daley CL, Krensky AM, Clayberger C IL-24 modulates IFN-gamma expression in patients with tuberculosis. Immunol. Lett., 2007-12-26;117(1):57-62. 2007-12-26 [PMID: 18199488] (Bioassay, Human)
• Tahara I, Miyake K, Hanawa H, Kurai T, Hirai Y, Ishizaki M, Uchida E, Tajiri T, Shimada T Systemic cancer gene therapy using adeno-associated virus type 1 vector expressing MDA-7/IL24. Mol. Ther., 2007-06-05;15(10):1805-11. 2007-06-05 [PMID: 17551500] (ELISA (Standard))
• Sa SM, Valdez PA, Wu J The effects of IL-20 subfamily cytokines on reconstituted human epidermis suggest potential roles in cutaneous innate defense and pathogenic adaptive immunity in psoriasis. J. Immunol., 2007-02-15;178(4):2229-40. 2007-02-15 [PMID: 17277128] (Bioassay, Human)

Bioinformatics

• Gene Symbol: IL24
• Uniprot:
  • Q13007()