Recombinant Human IL-12 R beta 1 Fc Avi-tag Protein, CF

When Recombinant Human IL-12 (Catalog # 219-IL) is immobilized at 1 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human IL-12 beta 1 Fc Chimera Avi-tag (Catalog # AVI10382) that produces 50% of the optimal binding response if found to be approximately 15‑90 ng/mL.

2 μg/lane of Biotinylated Recombinant Human IL-12 R beta 1 Fc Chimera Avi-tag (Catalog # AVI10382) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 95-115 kDa and 180-230 kDa, respectively.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human IL-12 R beta 1 Fc Avi-tag Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human IL-12 (Catalog # 219-IL) is immobilized at 1 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human IL-12 beta 1 Fc Chimera Avi-tag (Catalog # AVI10382) that produces 50% of the optimal binding response is found to be approximately 15-90 ng/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived human IL-12 R beta 1 protein

  Human IL-12 R beta 1 (Cys24-Glu540) Accession # P42701-1

  IEGRMD

  Human IgG 1 (Pro100-Lys330)

  Avi-tag

  N-terminus

  C-terminus

• Accession #: P42701-1
• N-terminal Sequence: Cys24
• Structure / Form: Disulfide-linked homodimer, biotinylated via Avi-tag
• Protein/Peptide Type: Recombinant Proteins
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Endotoxin Note: <1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 85 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 95-115 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 3 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Reconstitution Instructions: Reconstitute at 200 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-12 R beta 1 Fc Avi-tag Protein, CF

• CD212 antigen
• CD212
• IL-12 R beta 1
• IL-12 receptor beta component
• IL-12 receptor subunit beta-1
• IL12R beta 1
• IL-12R subunit beta-1
• IL12R
• IL12RB
• IL12RB1
• IL-12Rb1
• IL-12R-BETA1
• IL-12R-beta-1
• interleukin 12 receptor, beta 1
• interleukin-12 receptor beta-1 chain
• interleukin-12 receptor subunit beta-1
• MGC34454

Background

IL12RB1 (Interleukin-12 receptor subunit beta-1; IL-12 R beta 1), also known as CD212, is a type I transmembrane protein with similarities with the gp130/G-CSF R subgroup in the cytokine receptor superfamily. IL12RB1, along with IL12RB2, are two receptor subunits for IL12. Individually, the two subunits bind IL12 with low affinity, but when combined they form a high-affinity receptor complex (1). Human IL12RB1 contains 5 fibronectin domains in the extracellular domain (ECD), a single transmembrane domain and a box 1 motif in the cytoplasmic region. The mature ECD of human IL12RB1 shares 51% amino acid identity with mouse and rat IL12RB1. Interleukin 12 (IL12) is a pro-inflammatory cytokine and key mediator of cellular-immunity and induces the differentiation of Th1 cells from precursor T helper cells (2). Binding of IL12RB1, along with IL12RB2, is required for IL12 signal transduction and subsequent activity (1,2). Expression of IL12RB1 been detected in activated T cells, NK cells and B cells (1). The expression of IL12RB2 is more restricted and appears to be limited to Th2 cells. IL12RB1 has been reported to interact with IL23R to form the functional receptor complex for IL23 and probably activation of the Jak-Stat signaling cascade (3).

1. Robinson R.T. (2015) Cytokine. 71:348.
2. Rosenzweig S.D. and Holland S.M. (2005) Immunol. Rev. 203:38.
3. Zou J. et al. (1997) J. Biol Chem, 272:6073.

Bioinformatics

• Uniprot:
  • P42701-1()