Recombinant Human IGSF4B/SynCAM3 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human IGSF4B/SynCAM3 Protein, CF Summary

• Details of Functionality: Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Able to significantly enhance neurite outgrowth when immobilized as a 3 µL droplet containing 50 ng on a nitrocellulose-coated microplate.
• Source: Mouse myeloma cell line, NS0-derived human IGSF4B/SynCAM3 protein
  Pro21-Tyr329 & Asn25-Tyr329, both with a C-terminal 6-His tag
• Accession #: Q8N126
• N-terminal Sequence: Pro21 & Asn25
• Protein/Peptide Type: Recombinant Proteins
• Gene: CADM3
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 34.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 38-41 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IGSF4B/SynCAM3 Protein, CF

• BIgR
• Brain immunoglobulin receptor
• CADM3
• cell adhesion molecule 3
• IGSF4B
• IGSF4BSynCAM3
• Immunoglobulin superfamily member 4B
• member 4B
• Necl-1
• NECL1TSLC1-like protein 1
• Nectin-like protein 1
• Synaptic cell adhesion molecule 3
• SynCAM3
• TSLC1-like 1
• TSLL1
• TSLL1SYNCAM3

Background

IGSF4B (immunoglobulin superfamily member 4B), also called CADM3, TSLL1, Necl-1 and SynCAM-3, is a neural tissue-specific member of the nectin-like family of immunoglobulin superfamily (1, 2). It is a 48 - 50 kDa type I transmembrane (TM) glycoprotein that is concentrated at non-junctional contact sites of neuronal axons and glial processes (2, 3). In myelinated peripheral nerve fibers, IGSF4B is concentrated at nodes of Ranvier in contact sites of Schwann cells (2). The 398 amino acid (aa) human IGSF4B contains a 24 aa signal sequence, a 306 aa extracellular domain (ECD), a 21 aa transmembrane domain and a 47 aa cytoplasmic domain. The ECD contains an N-terminal V-type Ig-like domain that is responsible for Ca⁺⁺‑independent homophilic and heterophilic interactions with Nectin-1, Nectin-3 or Necl-2 (IGSF-4) in trans. It also contains two C2‑type Ig-like domains that are responsible for Ca⁺⁺-independent homophilic dimerization in cis that is thought to precede trans interaction (2, 4, 5). The cytoplasmic domain binds members of the MAGUK guanylate kinase subfamily, such as Dlg3, Pals2 and CASK (2). These activities are thought to play roles in adhesion and architecture at the synapse (2). We have also shown that IGSF4B promotes in vitro outgrowth of cortical neurons. Of two known splice variants, one shows an insertion of 34 aa near the mature N-terminus and probably represents a 60 kDa form. A smaller variant shows deletions of aa 185 - 231, and 281 - 398, which includes the first C2‑type Ig-like domain and the TM segment. The IGSF4B ECD is highly conserved, sharing 95 - 96% aa identity between human mouse, rat, canine and bovine sequences. The ECD of IGSF4A, B, C and D proteins share 35 - 50% aa identity.

1. Fukuhara, H. et al. (2001) Oncogene 20:5401.
2. Kakunaga, S. et al. (2005) J. Cell Sci. 118:1267.
3. Fukami, T. et al. (2003) Gene 323:11.
4. Shingai, T. et al. (2003) J. Biol. Chem. 278:35421
5. Dong, X. et al. (2006) J. Biol. Chem. 281:10610.

Bioinformatics

• Gene Symbol: CADM3
• Uniprot:
  • Q8N126()