Recombinant Human His6-USP30 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human His6-USP30 Protein, CF Summary

• Details of Functionality:

  Reaction conditions will need to be optimized for each specific application. We recommend an initial USP30 concentration of 20-100 nM in reactions utilizing Ubiquitin-AMC or Ubiquitin-Rhodamine substrates (U-550 or U-555), or 200-500 nM when digesting recombinant Polyubiquitin chain substrates.

• Source: Spodoptera frugiperda, Sf 21 (baculovirus)-derived human USP30 protein
  Thr57 - Glu517 with a N-terminal Met and a C-terminal 6-His tag
• Accession #: Q70CQ3.1
• Protein/Peptide Type: Recombinant Enzymes
• Gene: USP30
• Purity: >98%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 54 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
• Buffer: Supplied as a solution in HEPES, NaCl, Glycerol and DTT.
• Purity: >98%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human His6-USP30 Protein, CF

• Deubiquitinating Enzyme 30
• EC 3.1.2.15
• EC 3.4.19.12
• FLJ40511
• MGC10702
• ubiquitin carboxyl-terminal hydrolase 30
• ubiquitin specific peptidase 30
• ubiquitin specific protease 30
• Ubiquitin Thioesterase 30
• Ubiquitin thiolesterase 30
• ubiquitin-specific protease 30
• Ubiquitin-specific-processing protease 30
• Ub-specific protease 30
• USP30

Background

Ubiquitin carboxyl-terminal hydrolase 30 (USP30) is a member of the C19 family of peptidases and is tethered to the outer mitochondrial surface via a trans-membrane domain. USP30 activity is antagonistic to the pro-mitophagy signal generated by the activities of E3 Ubiquitin Ligase Parkin (PARK2) and kinase PINK1, which are stimulated by mitochondrial damage. In vivo, USP30 cleaves Ubiquitin from mitochondrial surface proteins such as MIRO1, TOMM20, MFN1, and MFN2. The enzyme displays a strong preference for K6- and K11-linked Polyubiquitin chains in vitro, but has substantially lower activity on Polyubiquitin chains that have been phosphorylated with PINK1. 

1. Bingol B. et al. (2014) Nature 510: 370.
2. Cunningham C.N. et al. (2015) Nat Cell Biol. 17: 160.
3. Thobois S. (2015) Mov. Disord. 30: 340.
4. Wang, Y. et al. (2015) Autophagy 11: 595.
5. Wauer T., et al. (2015) EMBO J. 34: 307.
6. Yue, W. et al. (2014) Cell Res. 24: 482.

Publications for USP30 (E-582)(3)

Publications using E-582

• Jadhav, P;Liang, X;Ansari, A;Tan, B;Tan, H;Li, K;Chi, X;Ford, A;Ruiz, FX;Arnold, E;Deng, X;Wang, J; Design of quinoline SARS-CoV-2 papain-like protease inhibitors as oral antiviral drug candidates Nature communications 2025-02-13 [PMID: 39948104] (Bioassay, N/A)
• Fang, TZ;Sun, Y;Pearce, AC;Eleuteri, S;Kemp, M;Luckhurst, CA;Williams, R;Mills, R;Almond, S;Burzynski, L;Márkus, NM;Lelliott, CJ;Karp, NA;Adams, DJ;Jackson, SP;Zhao, JF;Ganley, IG;Thompson, PW;Balmus, G;Simon, DK; Knockout or inhibition of USP30 protects dopaminergic neurons in a Parkinson's disease mouse model Nature communications 2023-11-13 [PMID: 37957154] (Bioassay, N/A)
• Tsefou E, Walker A, Clark E, Hicks A, Luft C, Takeda K, Watanabe T, Ramazio B, Staddon J, Briston T, Ketteler R Investigation of USP30 inhibition to enhance Parkin-mediated mitophagy: tools and approaches. Biochem J, 2021-12-10;478(23):4099-4118. 2021-12-10 [PMID: 34704599] (Bioassay, Human)

Bioinformatics

• Gene Symbol: USP30
• Uniprot:
  • Q70CQ3.1()