Recombinant Human His6-USP2 Catalytic Domain Protein, CF

• Reactivity: Hu
• Applications: Enzyme Activity
• Format: Carrier-Free

Recombinant Human His6-USP2 Catalytic Domain Protein, CF Summary

• Additional Information: Will be discontinued when existing inventory is gone.
• Details of Functionality: Recombinant Human USP2- His6 Catalytic Domain is a Ubiquitin-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human USP2- His6 Catalytic Domain concentration of 1-5 nM.
• Source: E. coli-derived human USP2 protein
  Asn259 - Met605 with a C-terminal 6-His tag
• Accession #: O75604.2
• Protein/Peptide Type: Recombinant Enzymes
• Gene: USP2
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Applications/Dilutions

• Dilutions:
  • Enzyme Activity
• Theoretical MW: 41 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
• Buffer: Supplied as a solution in HEPES, NaCl and TCEP.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human His6-USP2 Catalytic Domain Protein, CF

• Deubiquitinating enzyme 2
• EC 3.1.2.15
• EC 3.4.19.12,41 kDa ubiquitin-specific protease
• ubiquitin specific peptidase 2
• ubiquitin specific protease 12
• ubiquitin specific protease 2
• ubiquitin specific protease 9
• ubiquitin thioesterase 2
• Ubiquitin thiolesterase 2
• Ubiquitin-specific-processing protease 2
• UBP41
• UBP41ubiquitin carboxyl-terminal hydrolase 2
• USP2
• USP9

Background

Ubiquitin Specific Peptidase 2 (USP2), also known as UBP41 and USP9, is a cytoplasmic protein that belongs to the peptidase C19 family of deubiquitinating enzymes (1). It is widely expressed and serves to deubiquitinate select target substrates. At least three isoforms have thus far been described: USP2a, also known as USP2-69, USP-41, and USP2-45 (2,3). All isoforms contain the same catalytic core, but show variations within their N- and C-terminal domains (3). USP2a is the canonical isoform. It is 605 amino acids (aa) in length and has a predicted molecular weight of 68.1 kDa. Human USP2a shares 90% aa sequence identity with the mouse and rat orthologs. USP-41 and USP-45 are 362 and 396 aa in length, respectively, and have predicted molecular weights of 41.6 and 45.2 kDa. USP-41 and USP2a have been shown to be critical regulators of apoptosis and cell proliferation. They deubiquitinate poly-ubiquitinated target proteins, such as FAS, MDM2, and Cyclin D1, to promote or block apoptosis, depending on the context (3-9). USP-45, whose expression appears to be regulated by circadian and nutritional cues, is thought to regulate hepatic gluconeogenesis and glucose metabolism (10,11). This recombinant human protein encompasses the catalytic domain of USP2.

1. Baek, S.H. et al. (1997) J. Biol. Chem. 272:25560.
2. Gousseva, N. & R.T. Baker (2003) Gene Expr. 11:163.
3. Haimerl, F. et al. (2009) J. Biol. Chem. 284:495.
4. Gewies, A. & S. Grimm (2003) Cancer Res. 63:682.
5. Graner, E. et al. (2004) Cancer Cell 5:253.
6. Stevenson, L.F. et al. (2007) EMBO J. 26:976.
7. Shan, J. et al. (2009) Mol. Cell 36:469.
8. Mahul-Mellier, A.L. et al. (2012) Cell Death Differ. 19:891.
9. Mahul-Mellier, A.L. et al. (2012) Biochim. Biophys. Acta. 1823:1353.
10. Molusky, M.M. et al. (2012) Diabetes 61:1025.
11. Molusky, M.M. et al. (2012) PLoS One 7:e47970.

Publications for USP2 (E-506)(3)

Publications using E-506

• TET Mevissen, AV Prasad, JC Walter TRIM21-dependent target protein ubiquitination mediates cell-free Trim-Away Cell Reports, 2023-02-18;42(2):112125. 2023-02-18 [PMID: 36807144] (Bioassay, N/A)
• S Yoon, K Bogdanov, D Wallach Site-specific ubiquitination of MLKL targets it to endosomes and targets Listeria and Yersinia to the lysosomes Cell Death and Differentiation, 2022-01-09;0(0):. 2022-01-09 [PMID: 34999730] (Bioassay, Human)
• E Naik, VM Dixit Usp9X Is Required for Lymphocyte Activation and Homeostasis through Its Control of ZAP70 Ubiquitination and PKC? Kinase Activity J Immunol, 2016-03-02;196(8):3438-51. 2016-03-02 [PMID: 26936881] (Bioassay, Human)

Bioinformatics

• Gene Symbol: USP2
• Uniprot:
  • O75604.2()