Recombinant Human His6-UBE2F Protein, CF

• Reactivity: Hu
• Applications: Enzyme Activity
• Format: Carrier-Free

Recombinant Human His6-UBE2F Protein, CF Summary

• Additional Information: Will be discontinued when existing inventory is gone.
• Details of Functionality: Recombinant Human His6-UBE2F is a member of the NEDD8-conjugating (E2) enzyme family that receives NEDD8 from a NEDD8-activating (E1) enzyme and subsequently interacts with a NEDD8 ligase (E3) to conjugate NEDD8 to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-UBE2F concentration of 0.1-1 μM.
• Source: E. coli-derived human UBE2F protein
  Met1 - Arg185 with a N-terminal 6-His tag
• Accession #: NP_542409.1
• Protein/Peptide Type: Recombinant Enzymes
• Gene: UBE2F
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Applications/Dilutions

• Dilutions:
  • Enzyme Activity
• Theoretical MW: 23 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
• Buffer: Supplied as a solution in HEPES, NaCl, TCEP and Glycerol.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human His6-UBE2F Protein, CF

• EC 6.3.2
• EC 6.3.2.-
• MGC18120
• NCE2
• NCE2Ubiquitin-conjugating enzyme E2 F
• NEDD8 carrier protein UBE2F
• NEDD8 conjugating enzyme
• NEDD8 protein ligase UBE2F
• NEDD8-conjugating enzyme 2
• NEDD8-conjugating enzyme UBE2F
• UBE2F
• ubiquitin-conjugating enzyme E2F (putative)

Background

Ubiquitin-conjugating Enzyme E2F (UBE2F) is a NEDD8-conjugating enzyme (E2) (1). It is a 185 amino acid (aa) protein with a predicted molecular weight of 21 kDa. The human protein exhibits approximately 96% aa sequence identity with its mouse and rat orthologs. UBE2F is involved the covalent attachment of NEDD8 to target proteins in a process termed neddylation. For example, UBE2F appears to participate in the neddyylation of Cullin 5 (1). Members of the Cullin family (Cullin 1-7) are scaffold proteins that can incorporate into multimolecular complexes termed Cullin RING Ubiquitin Ligases (CRLs) (2). CRLs make up the largest subset of Ubiquitin Ligases (E3), and their activity is promoted by neddylation of associated Cullin family proteins (3).

1. Huang, D.T. et al. (2009) Mol. Cell 33:483.
2. Sarikas, A. et al. (2011) Genome Biol. 12:220.
3. Duda, D.M. et al. (2011) Curr. Opin. Struct. Biol. 21:257.

Publications for UBE2F/NCE2 (E2-679)(1)

Publication using E2-679

• Iwatani, Y;Matsuoka, K;Ode, H;Kubota, M;Nakata, Y;Setoyama, Y;Kojima, K;Imahashi, M;Yokomaku, Y; The unique structure of the highly conserved PPLP region in HIV-1 Vif is critical for the formation of APOBEC3 recognition interfaces mBio 2025-01-21 [PMID: 39835817] (Bioassay, N/A)

Bioinformatics

• Gene Symbol: UBE2F
• Uniprot:
  • NP_542409.1(Human)