Recombinant Human FOLR3 Protein, CF

• Reactivity: Hu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Human FOLR3 Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Folic Acid-Bovine Serum Albumin is coated at 5 μg/mL (100 μL/well), the concentration of rhFOLR-3 that produces 50% of the optimal binding response is found to be approximately 0.2 -1 nM.
• Source: Mouse myeloma cell line, NS0-derived human FOLR3 protein
  Ala22-Ser245 & Ser26-Ser245 both with a C-terminal 6-His tag
• Accession #: NP_000795
• N-terminal Sequence: Ala22 & Ser26
• Protein/Peptide Type: Recombinant Proteins
• Gene: FOLR3
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 26 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 30-40 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human FOLR3 Protein, CF

• folate receptor 3 (gamma)
• Folate receptor 3
• folate receptor gamma
• FOLR3
• FR-G
• FR-gamma
• gamma-hFR

Background

Folate receptor 3 (FOLR3), also known as folate receptor gamma , is a 32 kDa protein that binds folic acid and reduced folates. Dietary folates are required for many key metabolic processes including nucleotide and methionine synthesis, the interconversion of glycine and serine, and histidine breakdown (1). Mature FOLR3 is a glycosylated protein that contains an imperfect carboxy terminal GPI anchor sequence (2, 3). A fraction of FOLR3 is GPI-linked to the cell surface, but the majority is released as a secreted protein (2). Mature human FOLR3 shares 74% and 83% amino acid (aa) sequence identity with FOLR1 and FOLR2, respectively. Orthologs of human FOLR3 have not been described in mouse or rat. A genetic polymorphism generates a truncated isoform that lacks the carboxy terminal 139 aa (2, 4). FOLR3 is expressed by cells of the myelocyte and B lymphocyte lineage as well as by various carcinomas (2, 3). FOLR3 is more highly expressed in ovarian carcinomas than in breast carcinomas or mesotheliomas (5). Unlike other folate receptors, FOLR3 does not exhibit stereoselectivity in the binding of folates (3).

1. Fowler, B. et al. (2001) Kidney Int. 59:S-221.
2. Shen, F. et al. (1994) Biochemistry 33:1209.
3. Shen, F. et al. (1995) Biochemistry 34:5660.
4. Wang, H. et al. (1998) Nucl. Acids Res. 26:2132.
5. Yuan, Y. et al. (2009) Hum. Pathol. May 18 epub.

Bioinformatics

• Gene Symbol: FOLR3
• Uniprot:
  • NP_000795()