Recombinant Human Flt-3 Ligand Protein

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1 µg/lane of Recombinant Human Flt-3 Ligand was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing major bands at 22‑26 kDa. The multiple bands are due to variable ...read more
Recombinant Human Flt-3 Ligand (Catalog # 308-FK) stimulates cell proliferation in the BaF3 mouse pro-B cell line transfected with mouse Flt-3. The ED50 for this effect is 0.2-1 ng/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

Order Details

Recombinant Human Flt-3 Ligand Protein Summary

Details of Functionality
Measured in a cell proliferation assay using BaF3 mouse pro‑B cells transfected with mouse Flt-3. The ED50 for this effect is 0.2-1 ng/mL.
The specific activity of Recombinant Human Flt-3 Ligand is approximately 1.2 x 103 U/μg, which is calibrated against recombinant human
Flt-3 Ligand WHO Standard (NIBSC code: 96/532).
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Flt-3 Ligand protein
Thr27-Pro185
Accession #
N-terminal Sequence
Thr27
Protein/Peptide Type
Recombinant Proteins
Gene
FLT3LG
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
17.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
17-30 kDa, reducing conditions
Publications
Read Publications using
308-FK in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Flt-3 Ligand Protein

  • FL
  • Flt3 ligand
  • Flt-3 Ligand
  • Flt3L
  • FLT3LG
  • fms-related tyrosine kinase 3 ligand
  • SL cytokine

Background

Flt‑3 Ligand, also known as FL, is an alpha -helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages (1-3). Mature human Flt‑3 Ligand consists of a 158 amino acid (aa) extracellular domain (ECD) with a cytokine-like domain and a juxtamembrane tether region, a 21 aa transmembrane segment, and a 30 aa cytoplasmic tail (4-7). Within the ECD, human Flt‑3 Ligand shares 71% and 65% aa sequence identity with mouse and rat Flt‑3 Ligand, respectively. Human and mouse Flt‑3 Ligand show cross-species activity (4-6). Flt‑3 Ligand is expressed as a noncovalently-linked dimer by T cells and bone marrow and thymic fibroblasts (1, 8). Each 36 kDa chain carries approximately 12 kDa of N- and O-linked carbohydrates (8). Alternate splicing and proteolytic cleavage of the transmembrane form can generate a soluble 30 kDa fragment that includes the cytokine domain (4, 8). Alternate splicing of human Flt‑3 Ligand also generates membrane-associated isoforms that contain either a truncated cytoplasmic tail or an 85 aa substitution following the cytokine domain (4, 5, 8). Both transmembrane and soluble Flt‑3 Ligand signal through the tyrosine kinase receptor Flt-3/Flk-2 (3, 4, 6, 7). Flt‑3 Ligand induces the expansion of monocytes and immature dendritic cells as well as early B cell lineage differentiation (2, 9). It synergizes with IL-3, GM-CSF, and SCF to promote the mobilization and myeloid differentiation of hematopoietic stem cells (4-6). It cooperates with IL-2, -6, -7, and -15 to induce NK cell development and with IL-3, -7, and -11 to induce terminal B cell maturation (1, 10). Animal studies also show
Flt‑3 Ligand to reduce the severity of experimentally induced allergic inflammation (11).

  1. Wodnar-Filipowicz, A. (2003) News Physiol. Sci. 18:247.
  2. Dong, J. et al. (2002) Cancer Biol. Ther. 1:486.
  3. Gilliland, D.G. and J.D. Griffin (2002) Blood 100:1532.
  4. Hannum, C. et al. (1994) Nature 368:643.
  5. Lyman, S.D. et al. (1994) Blood 83:2795.
  6. Lyman, S.D. et al. (1993) Cell 75:1157.
  7. Savvides, S.N. et al. (2000) Nat. Struct. Biol. 7:486.
  8. McClanahan, T. et al. (1996) Blood 88:3371.
  9. Diener, K.R. et al. (2008) Exp. Hematol. 36:51.
  10. Farag, S.S. and M.A. Caligiuri (2006) Blood Rev. 20:123.
  11. Edwan, J.H. et al. (2004) J. Immunol. 172:5016.

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308-FK
Species: Hu
Applications: Bioactivity

Publications for Flt-3 Ligand (308-FK)(36)

We have publications tested in 6 confirmed species: Human, Mouse, Primate - Baboon, Primate - Callitrix jacchus (Common Marmoset), Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Primate - Macaca mulatta (Rhesus Macaque).

We have publications tested in 2 applications: Bioassay, cell culture.


Filter By Application
Bioassay
(32)
cell culture
(2)
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Human
(25)
Mouse
(4)
Primate - Baboon
(1)
Primate - Callitrix jacchus (Common Marmoset)
(1)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
(1)
Primate - Macaca mulatta (Rhesus Macaque)
(2)
All Species
Showing Publications 1 - 10 of 36. Show All 36 Publications.
Publications using 308-FK Applications Species
A Camgoz, M Paszkowski, S Satpathy, M Wermke, MV Hamann, M von Bonin, C Choudhary, S Knapp, F Buchholz STK3 is a therapeutic target for a subset of acute myeloid leukemias Oncotarget, 2018;9(39):25458-25473. 2018 [PMID: 29876001] (Bioassay, Human) Bioassay Human
LB Crawford, JH Kim, D Collins-Mc, BJ Lee, I Landais, C Held, JA Nelson, AD Yurochko, P Caposio Human Cytomegalovirus Encodes a Novel FLT3 Receptor Ligand Necessary for Hematopoietic Cell Differentiation and Viral Reactivation MBio, 2018;9(2):. 2018 [PMID: 29691342] (Bioassay, Human) Bioassay Human
M Cesana, MH Guo, D Cacchiarel, L Wahlster, J Barragan, S Doulatov, LT Vo, B Salvatori, C Trapnell, K Clement, P Cahan, KM Tsanov, PM Sousa, B Tazon-Vega, A Bolondi, FM Giorgi, A Califano, JL Rinn, A Meissner, JN Hirschhorn, GQ Daley A CLK3-HMGA2 Alternative Splicing Axis Impacts Human Hematopoietic Stem Cell Molecular Identity throughout Development Cell Stem Cell, 2018;22(4):575-588.e7. 2018 [PMID: 29625070] (Bioassay, Human) Bioassay Human
A Palau, AK Garz, J Diesch, A Zwick, R Malinverni, V Valero, K Lappin, R Casquero, A Lennartsso, J Zuber, T Navarro, KI Mills, KS Götze, M Buschbeck Polycomb protein RING1A limits hematopoietic differentiation in myelodysplastic syndromes Oncotarget, 2017;8(70):115002-115017. 2017 [PMID: 29383137] (Bioassay, Human) Bioassay Human
M Carretta, AZ Brouwers-V, M Bosman, SJ Horton, JHA Martens, E Vellenga, JJ Schuringa BRD3/4 inhibition and FLT3-ligand deprivation target pathways that are essential for the survival of human MLL-AF9+ leukemic cells PLoS ONE, 2017;12(12):e0189102. 2017 [PMID: 29240787] (Bioassay, Human) Bioassay Human
J Laiño, A Wangorsch, F Blanco, S Wolfheimer, M Krause, A Flaczyk, TM Möller, M Tsai, S Galli, S Vieths, M Toda, S Scheurer, S Schülke Targeting of Immune Cells by Dual TLR2/7 Ligands Suppresses Features of Allergic Th2 Immune Responses in Mice J Immunol Res, 2017;2017(0):7983217. 2017 [PMID: 29204451] (Bioassay, Mouse) Bioassay Mouse
X Jiang, JS Hawkins, J Lee, CO Lizama, FL Bos, JP Zape, P Ghatpande, Y Peng, J Louie, G Lagna, AC Zovein, A Hata Let-7 microRNA-dependent control of leukotriene signaling regulates the transition of hematopoietic niche in mice Nat Commun, 2017;8(1):128. 2017 [PMID: 28743859] (Bioassay, Mouse) Bioassay Mouse
AG Kotini, CJ Chang, A Chow, H Yuan, TC Ho, T Wang, S Vora, A Solovyov, C Husser, M Olszewska, J Teruya-Fel, D Perumal, VM Klimek, A Spyridonid, RK Rampal, L Silverman, EP Reddy, E Papaemmanu, S Parekh, BD Greenbaum, CS Leslie, MG Kharas, EP Papapetrou Stage-Specific Human Induced Pluripotent Stem Cells Map the Progression of Myeloid Transformation to Transplantable Leukemia Cell Stem Cell, 2017;0(0):. 2017 [PMID: 28215825] (Bioassay, Human) Bioassay Human
I Baccelli, J Krosl, G Boucher, I Boivin, VP Lavallée, J Hébert, S Lemieux, A Marinier, G Sauvageau A novel approach for the identification of efficient combination therapies in primary human acute myeloid leukemia specimens Blood Cancer J, 2017;7(2):e529. 2017 [PMID: 28211886] (Bioassay, Human) Bioassay Human
Pentraxin-2 suppresses c-Jun/AP-1 signaling to inhibit progressive fibrotic disease JCI Insight, 2016;1(20):e87446. 2016 [PMID: 27942582] (Bioassay, Human) Bioassay Human
Show All 36 Publications.

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Bioinformatics

Gene Symbol FLT3LG
Uniprot