Recombinant Human Fc epsilon RI alpha Protein, CF

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When Recombinant Human Fc epsilon RI alpha (6678-FC) is immobilized at 0.1 μg/mL (100 μL/well), the concentration of human IgE that produces 50% of the optimal binding response is found to be approximately ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human Fc epsilon RI alpha Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA.

When Recombinant Human Fc epsilon  RI alpha is immobilized at 0.1 μg/mL (100 μL/well), the concentration of human IgE that produces 50% of the optimal binding response is found to be approximately 0.0200-0.200 μg/mL.

Source
Mouse myeloma cell line, NS0-derived human Fc epsilon RI alpha protein
Val26-Gln205, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Val26
Protein/Peptide Type
Recombinant Proteins
Gene
FCER1A
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
21.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
45-58 kDa, reducing conditions
Publications
Read Publication using
6678-FC in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Fc epsilon RI alpha Protein, CF

  • Fc epsilon RI alpha
  • FCE1A
  • FCER1A
  • FcERI
  • FceRIa

Background

The alpha subunit of the high affinity IgE receptor (Fc epsilon  RI alpha or Fc epsilon RIA) is an IgE‑binding type I transmembrane glycoprotein of the multichain immune recognition (MIRR) family (1, 2). The receptor, Fc epsilon  RI, is a tetrameric complex of one alpha , one beta and two gamma subunits ( alpha beta gamma 2) on mast cells and basophils (1). An alternate trimeric form ( alpha gamma 2) is expressed on human, but not rodent, mast cells, basophils, eosinophils and professional antigen presenting cells (3). While the gamma subunit is essential for expression of Fc epsilon  RI alpha on the cell surface and for cell signaling, the beta subunit, when present, increases the halflife of the Fc epsilon  RI complex on the cell surface (3, 4). An isoform of the beta subunit, beta T, blocks processing of the alpha subunit and its cell surface expression (2, 3, 5). Human Fc epsilon  RI alpha cDNA encodes 257 amino acids (aa) including a 25 aa signal sequence, a 180 aa extracellular domain containing two Ig‑like domains that bind IgE and an endoplasmic reticulum retention motif, a 21 aa transmembrane domain with a charged amino acid (Asp219) that contributes to intracellular transport, and a 32 aa cytoplasmic sequence (1, 3, 6). Human Fc epsilon  RI alpha shares 50-62% aa sequence identity with mouse, rat, equine, ovine, bovine, porcine and canine Fc epsilon  RI alpha . Binding of IgE alone increases surface expression of Fc epsilon  RI, while crosslinking of IgE/Fc epsilon  RI complexes by IgE ligands (allergens) initiates receptor internalization and signaling (2, 4, 5). Mast cell and basophil activation by IgE/Fc epsilon  RI crosslinking causes degranulation, releasing histamine, leukotrienes, prostaglandins, and other mediators of immediate‑type and late‑phase allergic reactions. Circulating autoantibodies that crosslink Fc epsilon  RI alpha are often found in patients with chronic urticaria (7). Fc epsilon  RI on human antigen presenting cells mediates uptake and processing of allergens for presentation by class II MHC (2, 3). Fc epsilon  RI expression on human DC and Langerhans cells is up‑regulated during allergic reactions (atopy) and correlates with serum IgE concentration (3).

  1. Shimizu, A. et al. (1988) Proc. Natl. Acad. Sci. USA 85:1907.
  2. Abramson, J. and I. Pecht (2007) Immunol. Rev. 217:231.
  3. Kraft, S. and J-P. Kinet (2007) Nat. Rev. Immunol. 7:365.
  4. Yamasaki, S. and T. Saito (2008) J. Pharmacol. Sci. 106:336.
  5. Brenzovich, J. et al. (2009) J. Leukoc. Biol. 86:1351.
  6. Cauvi, D.M. et al. (2006) J. Biol. Chem. 281:10448.
  7. Kikuchi, Y. et al. (2001) J. Allergy Clin. Immunol. 107:1056.

Publications for Fc epsilon RI alpha (6678-FC)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Bioassay.


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Bioinformatics

Gene Symbol FCER1A
Uniprot