Recombinant Human ESAM Fc Chimera Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human ESAM Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its ability to induce ERK phosphorylation in BaF3 mouse pro‑B cells transfected with human ESAM. Stimulation of 2.5 x 10⁵ serum-starved hESAM transfected BaF3 cells with 25 µg Recombinant Human ESAM Fc Chimera (Catalog # 2688-EC) for 30 minutes at 37° C leads to ERK phosphorylation.
• Source: Mouse myeloma cell line, NS0-derived human ESAM protein
  

  Human ESAM (Gln30 - Ala247) Accession # Q96AP7	IEGRMD	Human IgG1 (Pro100 - Lys330)
  N-terminus		C-terminus

• Accession #: Q96AP7
• N-terminal Sequence: No results obtained: Gln30 predicted
• Protein/Peptide Type: Recombinant Proteins
• Gene: ESAM
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 50.3 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 67-75 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human ESAM Fc Chimera Protein, CF

• 2310008D05Rik
• endothelial cell adhesion molecule
• endothelial cell-selective adhesion molecule
• ESAM
• HUEL (C4orf1)-interacting protein
• LP4791 protein
• W117m

Background

Endothelial cell-selective adhesion molecule (ESAM) is a 55 kDa type I transmembrane glycoprotein that belongs to the JAM family of immunoglobulin superfamily molecules (1, 2). Human ESAM is synthesized as a 390 amino acid (aa) protein composed of a 29 aa signal peptide, a 216 aa extracellular region, a putative 26 aa transmembrane segment, and a 119 aa cytoplasmic domain. The extracellular region contains one V-type and one C2-type Ig domain and is involved in homophilic adhesion (1). In the cytoplasmic domain, there is a docking site for the multifunctional adaptor protein MAGI-1 (3). The extracellular region of human ESAM shows 90%, 74%, 69% and 67% aa identity with monkey, canine, mouse and rat extracellular ESAM, respectively. ESAM is expressed on endothelial cells, activated platelets and megakaryocytes, and can be found associated with cell-to-cell junctions. Whether ESAM is restricted to a particular junctional type is not clear (1, 2). ESAM deficient mice have no defect in vascularization but do have reduced angiogenic potential. This may be due to a decreased migratory response to FGF-2 (4).

1. Hirata, K-I, et al. (2001) J. Biol. Chem. 276:16223.
2. Nasdala, I. et al. (2002) J. Biol. Chem. 277:16294.
3. Wegmann, F. et al. (2004) Exp. Cell Res. 300:121.
4. Ishida, T. et. al. (2003) J. Biol. Chem. 278:34598.

Bioinformatics

• Gene Symbol: ESAM
• Uniprot:
  • Q96AP7()