Recombinant Human DLL1 Fc Chimera Protein, CF

Recombinant Human DLL1 Fc Chimera (Catalog # 10184-DL) enhances Recombinant Human BMP‑2 (Catalog # 355-BM) induced alkaline phosphatase activity in the C3H10T1/2 mouse embryonic fibroblast cell line. The ED50 for this effect is 0.1-0.5 μg/mL.

2 μg/lane of Recombinant Human DLL1 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 88-98 kDa and 170-190 kDa, respectively .

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human DLL1 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. Nobta, M. et al. (2005) J. Biol. Chem. 280:15842. The ED₅₀ for this effect is 0.1-0.5 μg/mL.
• Source: Mouse myeloma cell line, NS0-derived human DLL1 protein
  

  Human DLL1 (Ser22-Glu537) Accession # O00548	IEGRMDP	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: O00548
• N-terminal Sequence: Ser22
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 82 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 88-98 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in HEPES and EDTA.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 250 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human DLL1 Fc Chimera Protein, CF

• delta (Drosophila)-like 1
• Delta 1
• Delta
• Delta1
• delta-like 1 (Drosophila)
• delta-like protein 1
• DL1
• DLL1
• Drosophila Delta homolog 1
• H-Delta-1

Background

Delta-like protein 1 (DLL1) is a 90-100 kDa type I transmembrane protein that belongs to the Delta/Serrate/Lag-2 (DSL) family of Notch ligands. Mature human DLL1 consists of a 528 amino acid (aa) extracellular domain (ECD) with one DSL domain and eight EGF-like repeats, a 23 aa transmembrane segment, and a 155 aa cytoplasmic domain (1). Within the ECD, human DLL1 shares 91% aa sequence identity with mouse and rat DLL1. It shares 26%, 37%, and 54% aa sequence identity with DLL2, 3, and 4, respectively. A 60 kDa ECD fragment released by ADAM9, 12, or 17 mediated proteolysis, promotes the proliferation of hematopoietic progenitor cells (2, 3). The residual membrane-bound portion of DLL1 can be cleaved by presenilin-dependent gamma -secretase, enabling the cytoplasmic domain to migrate to the nucleus (4). DLL1 localizes to adherent junctions on neuronal processes through its association with the scaffolding protein MAGI1 (5). DLL1 is widely expressed, and it plays an important role in embryonic somite formation, cochlear hair cell differentiation, plus B and T lymphocyte differentiation (6-11). The up-regulation of DLL1 in arterial endothelial cells following injury or angiogenic stimulation is central to postnatal arteriogenesis (12). DLL1 is also over-expressed in cervical carcinoma and glioma and contributes to tumor progression (1, 13). Soluble DLL-1 was shown to inhibit differentiation of hematopoietic precursor cells (14).

1. Gray, G.E. et al. (1999) Am. J. Pathol. 154:785.
2. Dyczynska, E. et al. (2007) J. Biol. Chem. 282:436.
3. Karanu, F.N. et al. (2001) Blood 97:1960.
4. Ikeuchi, T. and S.S. Sisodia (2003) J. Biol. Chem. 278:7751.
5. Mizuhara, E. et al. (2005) J. Biol. Chem. 280:26499.
6. Takahashi, Y. et al. (2003) Development 130:4259.
7. Teppner, I. et al. (2007) BMC Dev. Biol. 7:68.
8. Kiernan, A.E. et al. (2005) Development 132:4353.
9. Schmitt, T.M. and J.C. Zuniga-Pflucker (2002) Immunity 17:749.
10. Hozumi, K. et al. (2004) Nat. Immunol. 5:638.
11. Santos, M.A. et al. (2007) Proc. Natl. Acad. Sci. 104:15454.
12. Limbourg, A. et al. (2007) Circ. Res. 100:363.
13. Purow, B.W. et al. (2005) Cancer Res. 65:2353.
14. Han. W. et al. (2000) Blood. 95(5):1616.

Publications for DLL1 (10184-DL)(4)

Publications using 10184-DL

• Cho, S;Servián-Morilla, E;Garrido, VN;Rodriguez-Gonzalez, B;Yuan, Y;Cano, R;Rambhiya, AA;Darabi, R;Haltiwanger, RS;Paradas, C;Jafar-Nejad, H; The glycosyltransferase POGLUT1 regulates muscle stem cell development and maintenance in mice bioRxiv : the preprint server for biology 2024-11-25 [PMID: 39651163] (Bioassay, Mouse)
• Alve, S;Gramolelli, S;Jukonen, J;Juteau, S;Pink, A;Manninen, AA;Hänninen, S;Monto, E;Lackman, MH;Carpén, O;Saharinen, P;Karaman, S;Vaahtomeri, K;Ojala, PM; DLL4-Notch3-WNT5B axis mediates bi-directional pro-metastatic crosstalk between melanoma and lymphatic endothelial cells JCI insight 2023-11-16 [PMID: 37971882] (Bioassay, Human)
• K Matsumoto, V Kumar, S Varshney, AV Nairn, A Ito, F Pennarubia, KW Moremen, P Stanley, RS Haltiwange Fringe GlcNAc-transferases differentially extend O-fucose on endogenous NOTCH1 in mouse activated T cells The Journal of Biological Chemistry, 2022-05-25;0(0):102064. 2022-05-25 [PMID: 35623385] (Binding Assay, Human)
• Shogo Tamura, Masato Mukaide, Yumi Katsuragi, Wataru Fujii, Koya Odaira, Nobuaki Suzuki et al. Periosteum-derived podoplanin-expressing stromal cells regulate nascent vascularization during epiphyseal marrow development Journal of Biological Chemistry 2022-05-01 [PMID: 35304101] (Bioassay, Mouse)

Bioinformatics

• Uniprot:
  • O00548()