Recombinant Human Desmoglein-2 Fc Chimera Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Desmoglein-2 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of BUD‑8 human fibroblast cells. When 3 x 10⁴ cells are added to recombinant human Desmoglein‑2 coated plates (10 μg/mL, 100 μL/well), approximately 50%‑80% will adhere after 1 hour at 37°C. Optimal dilutions should be determined by each laboratory for each application.
• Source: Mouse myeloma cell line, NS0-derived human Desmoglein-2 protein
  

  Human Desmoglein-2 (Ala49-Gly608) Accession # CAA81226	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: CAA81226
• N-terminal Sequence: Ala49
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: DSG2
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 89 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 115 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Desmoglein-2 Fc Chimera Protein, CF

• ARVC10
• ARVD10
• Cadherin family member 5
• CDHF5
• CDHF5MGC117034
• CMD1BB
• desmoglein 2
• Desmoglein2
• Desmoglein-2
• DSG2
• HDGC
• MGC117036
• MGC117037

Background

Desmoglein-2 (DSG2) is a 160 kDa transmembrane glycoprotein in the cadherin family of calcium dependent adhesion molecules. It is a major protein component of desmosomal contacts between epithelial cells (1, 2). Mature human Desmoglein-2 consists of a 560 amino acid (aa) extracellular domain (ECD) with four cadherin domains, a 25 aa transmembrane segment, and a 484 aa cytoplasmic domain with six desmoglein repeats (3). Within the ECD, human Desmoglein-2 shares 81% aa sequence identity with mouse and rat Desmoglein-2. A 60 kDa ECD fragment can be proteolytically shed, and this is enhanced by EGF R activation (4‑6). The cytoplasmic domain is cleaved during apoptosis, leaving the transmembrane segment and ECD as a cell-associated fragment (7). Desmoglein-2 is widely expressed in epithelia and is required for maintenance of the epithelial barrier (1, 2, 8). It can be either up- or down-regulated in a variety of carcinomas during tumor progression (9). Desmoglein-2 also functions as a cell adhesion receptor for several adenovirus serotypes (10). This binding disrupts intercellular junctions between epithelial cells and promotes epithelial-mesenchymal transition (EMT) (10). Mutations in Desmoglein-2 cause cardiac dysfunction typical of arrythmogenic right ventricular cardiomyopathy (ARVC) (11).

1. Brooke, M.A. et al. (2012) J. Pathol. 226:158.
2. Thomason, H.A. et al. (2010) Biochem. J. 429:419.
3. Schafer, S. et al. (1994) Exp. Cell Res. 211:391.
4. Bech-Serra, J.J. et al. (2006) Mol. Cell. Biol. 26:5086.
5. Klessner, J.L. et al. (2009) Mol. Biol. Cell 20:328.
6. Ramani, V.C. et al. (2008) BMC Cancer 8:373.
7. Nava, P. et al. (2007) Mol. Biol. Cell 18:4565.
8. Schlegel, N. et al. (2010) Am. J. Physiol. Gastrointest. Liver Physiol. 298:G774.
9. Dusek, R.L. and L.D. Attardi (2011) Nat. Rev. Cancer 11:317.
10. Wang, H. et al. (2011) Nat. Med. 17:96.
11. Pilichou, K. et al. (2009) J. Exp. Med. 206:1787.

Publications for Desmoglein-2 (947-DM)(1)

Publication using 947-DM

• AT Baker, A Greenshiel, L Coughlan, JA Davies, H Uusi-Kertt, DK Cole, PJ Rizkallah, AL Parker Diversity within the adenovirus fiber knob hypervariable loops influences primary receptor interactions Nat Commun, 2019-02-14;10(1):741. 2019-02-14 [PMID: 30765704] (Surface Plasmon Resonance, Human)

Bioinformatics

• Gene Symbol: DSG2
• Uniprot:
  • CAA81226()