Recombinant Human DC-SIGNR/CD299 Fc Chimera Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human DC-SIGNR/CD299 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of ICAM-3 expressing CHO Chinese hamster ovary cells. When 5 x 10⁴ cells/well are added to rhDC-SIGNR/Fc Chimera coated plates (5 µg/mL with 100 µL/well), approximately 40%-70% of added cells will adhere after 1 hour at room temperature.
  Optimal dilutions should be determined by each laboratory for each application.
• Source: Mouse myeloma cell line, NS0-derived human DC-SIGNR/CD299 protein
  

  MD	Human IgG1 (Pro100-Lys330)	IEGR	Human DC-SIGNR (Ser73-Glu376) Accession # NP_001138382
  N-terminus			C-terminus

• Accession #: NP_001138382
• N-terminal Sequence: Met
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: CLEC4M
• Purity: >80%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 61.4 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 61-66 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Tris-Citrate.
• Purity: >80%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human DC-SIGNR/CD299 Fc Chimera Protein, CF

• CD209L
• CD209L1
• CD209Ldendritic cell-specific ICAM-3-grabbing nonintegrin 2
• CD299 antigenMGC129964
• CD299
• CLEC4M
• C-type lectin domain family 4, member M
• DC-SIGN2
• DC-SIGN2CD209 antigen-like protein 1
• DCSIGNR
• DC-SIGNR
• DC-SIGNRDC-SIGN-related protein
• DCSIGNRMGC47866
• Dendritic cell-specific ICAM-3-grabbing non-integrin 2
• HP10347
• liver/lymph node-specific ICAM-3 grabbing non-integrin
• Liver/lymph node-specific ICAM-3-grabbing non-integrin
• LSIGN
• L-SIGN
• LSIGNC-type lectin domain family 4 member M
• mannose binding C-type lectin DC-SIGNR

Background

Human Dendritic Cell-specific ICAM-3 Grabbing Non-integrin (DC-SIGN)/CD209 is a member of the C-type lectin family (1). The canonical DC-SIGN/CD209 isoform is a 46 kDa, 404 amino acid (aa) type II transmembrane protein (2). The extracellular region contains a Ca²⁺-dependent carbohydrate-binding lectin domain (2). Multiple human DC-SIGN/CD209 splice forms exist, generating both membrane-bound and soluble forms (3). DC-SIGN/CD209 is not well conserved between mouse and human, with the extracellular domain sharing only 63% aa identity. The DC-SIGN/CD209 lectin domain binds mannose oligosaccharides on pathogens including HIV as well as self glycoproteins including ICAMs (2, 4). DC-SIGN/CD209 is expressed on dendritic cells (DC) and inflammatory macrophages and contributes to antigen presentation (5, 6).

1. Liu, W. et al. (2004) J. Biol. Chem. 279:18748.
2. Curtis, B.M. et al. (1992) Proc. Natl. Acad. Sci. USA 89:8356.
3. Mummidi, S. et al. (2001) J. Biol. Chem. 276:33196.
4. Anthony, R.M. et al. (2008) Proc. Natl. Acad. Sci. USA 105:19571.
5. Geijtenbeek, T.B. et al. (2000) Cell 100:575.
6. Garcia-Vallejo, J.J. and Y. van Kooyk (2013) Trends Immunol. 34:482.

Publications for DC-SIGNR/CD299/CLEC4M (162-D2)(7)

Publications using 162-D2

• Lei Guo, Yan Liang, Heng Li, Huiwen Zheng, Zening Yang, Yanli Chen et al. Epigenetic glycosylation of SARS-CoV-2 impact viral infection through DC&L-SIGN receptors iScience 2021-12-17 [PMID: 34786539] (ELISA Capture, N/A)
• Z Zou, J Misasi, N Sullivan, PD Sun Overexpression of Ebola virus envelope GP1 protein Protein Expr. Purif., 2017-04-27;0(0):. 2017-04-27 [PMID: 28458053] (Enzyme Assay, Virus - Ebola)
• Y Zhang, Q Zhang, M Zhang, M Yuan, Z Wang, J Zhang, X Zhou, Y Zhang, F Lin, H Na, S Ren, Y Zuo DC - SIGNR by influencing the lncRNA HNRNPKP2 upregulates the expression of CXCR4 in gastric cancer liver metastasis Mol. Cancer, 2017-04-13;16(1):78. 2017-04-13 [PMID: 28403883] (ELISA (Standard), Human)
• Czajkowsky D, Andersen J, Fuchs A, Wilson T, Mekhaiel D, Colonna M, He J, Shao Z, Mitchell D, Wu G, Dell A, Haslam S, Lloyd K, Moore S, Sandlie I, Blundell P, Pleass R Developing the IVIG biomimetic, hexa-Fc, for drug and vaccine applications. Sci Rep, 2015-04-27;5(0):9526. 2015-04-27 [PMID: 25912958] (Bioassay)
• Jiang , Yanmei, Zhang , Changfu, Chen , Kai, Chen , Zhe, Sun , Zhigang, Zhang , Zhuqing, Ding , Dongbing, Ren , Shuangyi, Zuo , Yunfei The clinical significance of DC-SIGN and DC-SIGNR, which are novel markers expressed in human colon cancer. PLoS ONE, 2014-12-12;9(12):e114748. 2014-12-12 [PMID: 25504222] (ELISA (Standard), Human)
• Nandakumar , Subhadra, Kannanganat , Sunil, Dobos , Karen M, Lucas , Megan, Spencer , John S, Fang , Sunan, McDonald , Melissa, Pohl , Jan, Birkness , Kristin, Chamcha , Venkates, Ramirez , Melissa, Plikaytis , Bonnie B, Posey , James E, Amara , Rama Rao, Sable , Suraj B O-mannosylation of the Mycobacterium tuberculosis adhesin Apa is crucial for T cell antigenicity during infection but is expendable for protection. PLoS Pathog, 2013-10-10;9(10):e1003705. 2013-10-10 [PMID: 24130497] (Bioassay, Human)
• Johnson TR, McLellan JS, Graham BS Respiratory syncytial virus glycoprotein G interacts with DC-SIGN and L-SIGN to activate ERK1 and ERK2. J. Virol., 2011-11-16;86(3):1339-47. 2011-11-16 [PMID: 22090124] (Bioassay)

Bioinformatics

• Gene Symbol: CLEC4M
• Uniprot:
  • NP_001138382()