Recombinant Human CXADR Fc Chimera Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human CXADR Fc Chimera Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to support the adhesion of human monocyte-derived dendritic cells. When 5 x 104 cells/well are added to rhCXADR/Fc Chimera coated plates (10 µg/mL), approximately 25%‑45% will adhere after 30 minutes of incubation at 37° C.
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived human CXADR protein
Human CXADR
(Leu20 - Gly237)
Accession # P78310
IEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Leu20
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
CXADR
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
50.6 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
60-65 kDa, reducing conditions
Publications
Read Publications using
3336-CX in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CXADR Fc Chimera Protein, CF

  • CAR10Coxsackievirus B-adenovirus receptor
  • CAR4/6
  • coxsackie virus and adenovirus receptor
  • coxsackie virus B receptor
  • coxsackievirus and adenovirus receptor
  • CVB3 binding protein
  • CVB3 BP
  • CVB3-binding protein
  • CXADR
  • HCAR
  • HCVADR

Background

CXADR (coxsackie and adenovirus receptor), also known as CAR, is a 46 kDa type I transmembrane glycoprotein that belongs to the CTX family of the Ig superfamily (1 - 3). CXADR has received attention as a receptor that facilitates gene transfer mediated by most adenoviruses (1, 2). It is also an adhesion molecule within junctional complexes, notably between epithelial cells lining body cavities and within myocardial intercalated discs (1, 2, 4). CXADR is essential for normal cardiac development in the mouse (7). It is expressed throughout brain neuroepithelium during development, but mainly in ependymal cells in the adult (4 - 6). The 365 amino acid (aa) human CXADR contains a 19 aa signal sequence, a 218 aa extracellular domain (ECD) with a V-type (D1) and a C2-type (D2) Ig-like domain, a 21 aa transmembrane segment and a 107 aa intracellular domain. D1 is thought to be responsible for homodimer formation in trans within tight junctions (2). The fiber knob of adenoviruses attaches at a similar site, and evidence suggests that disruption of tight junctions facilitates virus binding (1, 2). The C-terminus interacts with several cytoplasmic junctional proteins, microtubules and the actin cytoskeleton (1, 8, 9). The ECD of human CXADR shares 90% aa sequence identity with mouse, rat, and porcine CXADR, and 92% and 89% aa identity with bovine and canine CXADR, respectively. An alternately spliced isoform (CXADR2) that diverges in the C-terminal 15 aa shows a similar expression pattern (4, 10). Transcription of splice variants that produce soluble forms of CXADR has been detected, and secreted forms in serum and pleural fluid potentially block viral infection (11).

  1. Coyne, C.B. and J.M. Bergelson (2005) Adv. Drug Deliv. Rev. 57:869.
  2. Philipson, L. and R.F. Pettersson (2004) Curr. Top. Microbiol. Immunol. 273:87.
  3. Tomko, R.P. et al. (1997) Proc. Natl. Acad. Sci. USA 94:3352.
  4. Raschperger, E. et al. (2006) Exp. Cell Res. 312:1566.
  5. Hotta, Y. et al. Dev. Brain Res. 143:1.
  6. Hauwel, M. et al. (2005) Brain Res. Rev. 48:265.
  7. Chen, J. et al. (2006) Circ. Res. 98:923.
  8. Fok, P.T. et al. (2007) J. Biol. Chem. 282:7512.
  9. Huang, K-C. et al. (2007) FEBS Lett. 581:2702.
  10. Mirza, M. et al. (2006) Exp. Cell Res. 312:817.
  11. Bernal, R.M. et al. (2002) Clin. Cancer Res. 8:1915.

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Publications for CXADR (3336-CX)(3)

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Bioinformatics

Gene Symbol CXADR
Uniprot