Recombinant Human CRACC/SLAMF7 Fc Chimera Protein, CF Summary
Details of Functionality |
Measured by its ability to inhibit anti-CD3 antibody induced IFN-gamma secretion by human peripheral blood mononuclear cells (PBMC). The ED50 for this effect is 1-6 μg/mL.
|
Source |
Human embryonic kidney cell, HEK293-derived human CRACC/SLAMF7 protein Human SLAMF7 (Ser23-Met226) Accession # Q9NQ25 | IEGRMD | Human IgG1 (Pro100-Lys330) | N-terminus | | C-terminus | |
|
Accession # |
|
N-terminal Sequence |
Ser23 |
Structure / Form |
Disulfide-linked homodimer
|
Protein/Peptide Type |
Recombinant Proteins |
Purity |
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
Endotoxin Note |
<0.10 EU per 1 μg of the protein by the LAL method. |
Applications/Dilutions
Theoretical MW |
49 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
SDS-PAGE |
65-75 kDa, reducing conditions
|
Packaging, Storage & Formulations
Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, ≤ -20 °C under sterile conditions after reconstitution.
|
Buffer |
Lyophilized from a 0.2 μm filtered solution in PBS. |
Purity |
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
Reconstitution Instructions |
Reconstitute at 100 μg/mL in PBS. |
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Recombinant Human CRACC/SLAMF7 Fc Chimera Protein, CF
Background
CD2-like
receptor activating cytotoxic cells (CRACC), also known as CS1, novel Ly9,
SLAMF7, and CD319, is a 65-75 kDa type I transmembrane glycoprotein in the SLAM subgroup of the CD2 family (1). Mature human CRACC consists of a
204 amino acid (aa) extracellular domain (ECD) with one Ig-like V-set
domain and one Ig-like C2-set domain, a 21 aa transmembrane segment, and an
88 aa cytoplasmic domain with one immunoreceptor tyrosine-based switch
motif (ITSM) (2, 3). Within the ECD, human CRACC shares 54% and 52% aa
sequence identity with mouse and rat CRACC, respectively. There are seven known
isoforms of CRACC which are distinguished by deletions and/or substitutions in
both their ECD and cytoplasmic domains. CRACC is expressed on the surface of NK
cells, CD8
+ T cells, activated B cells, and mature dendritic
cells (4, 5). Its homophilic interaction induces NK, CTL, and B cell
activation (4-7). In human NK cells, activated CRACC transmits signals
following association with the adaptor protein EAT-2 (8).
- Veillette, A. (2006) Immunol. Rev. 214:22.
- Tovar, V. et al. (2002) Immunogenetics 54:394.
- Murphy, J.J. et al. (2002) Biochem. J. 361:431.
- Bouchon, A. et al. (2001) J. Immunol. 167:5517.
- Lee, J.K. et al. (2007) J. Immunol. 179:4672.
- Kumaresan, P.R. et al. (2002) Mol. Immunol. 39:1.
- Stark, S. and C. Watzl (2006) Int. Immunol. 18:241.
- Tassi, H. and M. Colonna (2005) J. Immunol. 175:7996.
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