Recombinant Human Corneodesmosin Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human Corneodesmosin Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to support the adhesion of BUD‑8 human fibroblast cells. When 3 x 104 cells/well are added to recombinant human CDSN coated plates (10 μg/mL, 100 μL/well), approximately 50-80% will adhere after 1 hour at 37 °C.
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived human Corneodesmosin protein
Lys33-Pro528, with a C-terminal 6-His tag (Asp526Asn)
Accession #
N-terminal Sequence
Lys33
Protein/Peptide Type
Recombinant Proteins
Gene
CDSN
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
49.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
60 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Tris.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 200 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Corneodesmosin Protein, CF

  • CDSN
  • Corneodesmosin
  • D6S586E
  • differentiated keratinocyte S protein
  • HTSS
  • S protein
  • S

Background

Corneodesmosin, also known as CDSN and the S gene product, is a highly polymorphic secreted glycoprotein that plays an important structural role in the skin (1). It is expressed by differentiated keratinocytes in the corneal layer of the skin and is a major component of corneodesmosomes (2 - 4). It is also expressed in the inner root sheath of hair follicles (5, 6). Corneodesmosome has a high content of glycine, serine, and proline residues that promote its folding into a series of Gly-loop domains (2, 7). Corneodesmosin forms oligomers and associates homophilically to strengthen the adhesion between corneocytes (8, 9). Corneodesmosin-deficient mice exhibit a detachment of the corneal layer of the skin as well as hypotrichosis of the scalp and baldness (6, 10). Corneodesmosin is secreted by keratinocytes as a 52 - 56 kDa molecule which is then subjected to repeated sequential N- and C-terminal proteolysis (11). Species of 46, 43, 36, and 15 kDa are present in corneocytes (7, 11). Cleavage of the N-terminal Gly-loop diminishes Corneodesmosin’s ability to mediate adhesion, and this is a prerequisite for normal desquamation of the skin (8, 9). Reduced proteolysis of Corneodesmosin in psoriasis lesions is associated with the persistence of corneodesmosomes and scale retention (12). Premature truncation of Corneodesmosin is associated with hypotrichosis of the scalp (13).
  1. Pierard, G.E. et al. (2000) Int. J. Mol. Med. 6:217.
  2. Zhou, Y. and D.D. Chaplin (1993) Proc. Natl. Acad. Sci. 90:9470.
  3. Haftek, M. et al. (1997) Br. J. Dermatol. 137:864.
  4. Simon, M. et al. (1997) J. Biol. Chem. 272:31770.
  5. Mils, V. et al. (1992) J. Histochem. Cytochem. 40:1329.
  6. Matsumoto, M. et al. (2008) Proc. Natl. Acad. Sci. 105:6720.
  7. Guerrin, M. et al. (1998) J. Biol. Chem. 273:22640.
  8. Jonca, N. et al. (2002) J. Biol. Chem. 277:5024.
  9. Caubet, C. et al. (2004) J. Invest. Dermatol. 122:747.
  10. Leclerc, E.A. et al. (2009) J. Cell Sci. 122:2699.
  11. Simon, M. et al. (2001) J. Biol. Chem. 276:20292.
  12. Simon, M. et al. (2008) Br. J. Dermatol. 159:77.
  13. Levy-Nissenbaum, E. et al. (2003) Nat. Genet. 34:151.

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Bioinformatics

Gene Symbol CDSN
Uniprot